How iMALDI can improve clinical diagnostics
Protein mass spectrometry (MS) is an indispensable tool to detect molecular signatures that can be associated with cellular dysregulation and disease.
Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells
This review summarizes recent research progress in the development of micro/nanoelectrochemical probe and chip devices for the evaluation of three-dimensional (3D) cultured cells.
Recent advances in the chemical imaging of human fingermarks (a review)
This review highlights the considerable advances in the chemical imaging of human fingermarks. Additional information about the donor can be obtained from the chemical composition of latent fingermarks besides the physical pattern, such as the age, gender, medical history and possible drug habits.
Recent advancements in ion concentration polarization
Advancements in ion concentration polarization made over the past three years are highlighted.
Electrochemical hydrogen sulfide biosensors
Biological application of electrochemical hydrogen sulfide sensors.
Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics
We present an overview of current isolation, detection, and characterization methods of extracellular vesicles and their applications and limitations as a potential emerging biomarker in cancer management and their clinical implementation.
Thin film depth profiling by ion beam analysis
The analysis of thin films is of central importance for functional materials, including the very large and active field of nanomaterials.
High spatial and temporal resolution cell manipulation techniques in microchannels
Reviewing latest developments on lab on chips for enhanced control of cells’ experiments.
Gold nanoparticles as sensitive optical probes
Recent advances in Au NP based optical sensing systems for various analytes based on absorption, fluorescence and SERS are summarized.
Hybrid plasmonic–photonic whispering gallery mode resonators for sensing: a critical review
In this review we present the state of the art and the most recent advances in the field of optical sensing with hybrid plasmonic–photonic whispering gallery mode (WGM) resonators.
A critical insight into the development pipeline of microfluidic immunoassay devices for the sensitive quantitation of protein biomarkers at the point of care
A critical review on the development of microfluidic tests capable of the high-sensitivity measurement of protein biomarkers at the point-of-care.
Chemometrics for ion mobility spectrometry data: recent advances and future prospects
This is the first comprehensive review on chemometric techniques used in ion mobility spectrometry data analysis.
Nanoparticles and intracellular applications of surface-enhanced Raman spectroscopy
Surface-enhanced Raman spectroscopy offers ultra-sensitive vibrational fingerprinting within biological cells.
Raman spectroscopy: an evolving technique for live cell studies
This review highlights some of the most recent advances in the development of Raman spectroscopy for live cell studies.
Functionalization of metal nanoclusters for biomedical applications
Metal nanoclusters (NCs) are emerging as a new class of functional nanomaterials in the area of biological sensing, labelling, imaging and therapy due to their unique physical and chemical properties, such as ultrasmall size, HOMO–LUMO transition, strong luminescence together with good photostability and biocompatibility.
Micro-scale spatially offset Raman spectroscopy for non-invasive subsurface analysis of turbid materials
This article reviews a very recent field of noninvasive analysis of turbid media using micro-scale Spatially Offset Raman Spectroscopy – micro-SORS.
Biomarker detection technologies and future directions
Advancement in technologies which can provide highly selective and sensitive multiplex detection of biomarkers is inevitable for accurate disease prognosis, diagnosis, and treatment follow-up.
Engineering molecularly imprinted polymers (MIPs) for the selective extraction and quantification of the novel psychoactive substance (NPS) methoxphenidine and its regioisomers
The first developed Molecularly Imprinted Polymers (MIPs) for the specific detection of methoxphenidine (MXP) and its regioisomers.
Structure elucidation and quantification of the reduction products of anticancer Pt(IV) prodrugs by electrochemistry/mass spectrometry (EC-MS)
A novel analytical approach for the identification/quantification of the reduction products of platinum(IV) complexes is presented.
Bulk double emulsification for flow cytometric analysis of microfluidic droplets
We describe a simple bulk method to prepare double emulsions for high-throughput analysis and sorting with flow cytometers.
10 nm deep, sub-nanoliter fluidic nanochannels on germanium for attenuated total reflection infrared (ATR-IR) spectroscopy
Nanoslits with a depth of ∼10 nm were manufactured on a germanium internal reflection element for attenuated internal reflection infrared spectroscopy.
Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system
A quantitative method based on image analysis of multiple color changes in microfluidic paper-based analytical devices using CIE L*a*b* color difference is presented.
Next generation of labeling reagents for quantitative and multiplexing immunoassays by the use of LA-ICP-MS
A novel labeling strategy – which results in one label per antibody molecule – was used for multiplex and quantitative immuno imaging by use of LA-ICP-MS.
Advanced gas sensors based on substrate-integrated hollow waveguides and dual-color ring quantum cascade lasers
The first combination of a ring-shaped vertically emitting quantum cascade laser (riQCL) with a substrate-integrated hollow waveguide (iHWG) is presented.
Infrared spectroscopy via substrate-integrated hollow waveguides: a powerful tool in catalysis research
Monitoring catalyst performance via substrate-integrated hollow waveguide (iHWGs) assisted infrared spectroscopy.
Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors
Using Surface Enhanced Raman Spectroscopy to measure cell death caused by radiation in a 3D model of prostate cancer.
A tunable Au core–Ag shell nanoparticle tip for tip-enhanced spectroscopy
A single Au nanoparticle (NP) with a diameter of 5 nm was transferred to the end of a Si-tip through a picking process, and an Ag shell with a controlled thickness was formed on the Au core.
Detection of prostate cancer using a voltammetric electronic tongue
A multivariate analysis of data from urine with an electronic voltammetric tongue to detect patients with prostate cancer.
Electrocatalytic monitoring of peptidic proton-wires
The transfer of protons or proton donor/acceptor abilities is an important phenomenon in many biomolecular systems.
Signal enhancement in ligand–receptor interactions using dynamic polymers at quartz crystal microbalance sensors
The signal enhancement properties of QCM sensors based on dynamic, biotinylated poly(acrylic acid) brushes has been studied in interaction studies with an anti-biotin Fab fragment.
NMR detection in biofluid extracts at sub-μM concentrations via para-H2 induced hyperpolarization
A combination of solid phase extraction, para-hydrogen induced hyperpolarization and selective NMR detection allows the quantitative determination of a doping substance at sub-μM concentrations in urine.
The origin of the band at around 730 cm−1 in the SERS spectra of bacteria: a stable isotope approach
A stable isotope approach combined with SERS analysis of bacteria allows clarification of the origin of a pronounced band at 730 cm−1.
Analytically useful blue chemiluminescence from a water-soluble iridium(III) complex containing a tetraethylene glycol functionalised triazolylpyridine ligand
The first highly water soluble, blue-luminescent iridium(III) complex for chemiluminescence detection is presented.
Utilising copper screen-printed electrodes (CuSPE) for the electroanalytical sensing of sulfide
A mediatorless sulfide electrochemical sensing platform utilising a novel nanocopper-oxide screen-printed electrodes (CuSPE) is reported for the first time.
A ratiometric fluorescent probe for detection of biogenic primary amines with nanomolar sensitivity
An ultrasensitive ratiometric fluorescent sensor made of an N,N-dimethylaminonaphthalene anhydride moiety for detection of aliphatic primary amines is reported.
Matrix-assisted laser desorption/ionization tandem mass spectrometry for identification of organic tattoo pigments in inks and tissue samples
Highly specific identification of water-insoluble organic pigments is facilitated by automated matrix-assisted laser desorption/ionization tandem mass spectrometry and data evaluation.
Towards microbioprocess control: an inexpensive 3D printed microbioreactor with integrated online real-time glucose monitoring
A 3D printed micro-bioreactor and microfluidic chip with integrated screen printed glucose biosensor for online monitoring of glucose to aid micro-bioprocess control.
A novel, fast and sensitive supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) method for analysis of arachidonic acid metabolites
A validated method for rapid and reliable measurement of five arachidonic acid metabolites in biofluids using supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS).
An interdigitated electrode with dense carbon nanotube forests on conductive supports for electrochemical biosensors
A highly sensitive interdigitated electrode (IDE) with vertically aligned dense carbon nanotube forests directly grown on conductive supports was demonstrated by combining UV lithography and a low temperature chemical vapor deposition process (470 °C).
SABRE hyperpolarization enables high-sensitivity 1H and 13C benchtop NMR spectroscopy
Benchtop NMR spectrometers operating with magnetic fields of 1–2 T at sub-ppm resolution coupled with SABRE hyperpolarization show great promise as analytical platforms that can be used outside the traditional laboratory environment.
A facile electrochemical intercalation and microwave assisted exfoliation methodology applied to screen-printed electrochemical-based sensing platforms to impart improved electroanalytical outputs
An Electrochemical Derived Intercalation process is explored as a modification for screen-printed electrodes to improve their electroanalytical outputs.
Polydisperse emulsion digital assay to enhance time to detection and extend dynamic range in bacterial cultures enabled by a statistical framework
We have developed a simple method for preparing small-volume droplets for performing digital culture, allowing for the detection of bacteria.
Salient space detection algorithm for signal extraction from contaminated and distorted spectrum
The proposed algorithm minimizes the influence of baseline distortion and exhibits good anti-noise ability and high real-time performance.
Multimodal imaging of undecalcified tissue sections by MALDI MS and μXRF
Lipid and elemental distributions in chicken phalanx imaged by MALDI MSI and μXRF.
Simultaneous non-polar and polar lipid analysis by on-line combination of HILIC, RP and high resolution MS
Polar and non-polar lipids analysis in 15 min chromatographic run time was enabled by a novel instrumental setup using orthogonal HILIC and RP separation and effluent combination prior to high-resolution mass spectrometry.
Collection of amino acids and DNA from fingerprints using hydrogels
We detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids and DNA from fingerprints, while allowing for their increased visualisation at a later stage.
Fast selective trapping and release of picoliter droplets in a 3D microfluidic PDMS multi-trap system with bubbles
A surface acoustic wave (SAW) induces a bubble in a 3D designed multi-trap polydimethylsiloxane (PDMS) device for the selection, incubation and on-demand release of aqueous droplets from a continuous oil flow.
High temporal resolution delayed analysis of clinical microdialysate streams
This paper presents the use of tubing to store clinical microdialysis samples for delayed analysis with high temporal resolution, offering an alternative to traditional discrete offline microdialysis sampling. A model allowing optimal results is described.
Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling
A multiple-pass, exonuclease III-mediated target recycling circuit is described for sensitive DNA detection with a shorter assay time.
Imaging of compartmentalised intracellular nitric oxide, induced during bacterial phagocytosis, using a metalloprotein–gold nanoparticle conjugate
Imaging of the in situ production of nitric oxide following phagocytosis of Escherichia coli bacteria using a NO nanobiosensor.
Cellulose ionics: switching ionic diode responses by surface charge in reconstituted cellulose films
Cellulose films as well as chitosan-modified cellulose films of approximately 5 μm thickness, reconstituted from ionic liquid media onto a poly(ethylene-terephthalate) (PET, 6 μm thickness) film with a 5, 10, 20, or 40 μm diameter laser-drilled microhole, show significant current rectification in aqueous NaCl.
Titanium nanoparticles (TiO2)/graphene oxide nanosheets (GO): an electrochemical sensing platform for the sensitive and simultaneous determination of benzocaine in the presence of antipyrine
An effective electrochemical sensing platform for the simultaneous determination of benzocaine (BEN) and antipyrine (ANT).
Spatially offset Raman spectroscopy for photon migration studies in bones with different mineralization levels
This study provides a deeper understanding of bone's optical properties which is essential to the development of SORS-based diagnostic tools.
Understanding the metabolism of the anticancer drug Triapine: electrochemical oxidation, microsomal incubation and in vivo analysis using LC-HRMS
The metabolism of the anticancer thiosemicarbazone Triapine was investigated.
Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231
1 step of PBS wash followed by quenching with 60% methanol supplemented with 70 mM HEPES results in minimal metabolite leakage.
LILY-lipidome isotope labeling of yeast: in vivo synthesis of 13C labeled reference lipids for quantification by mass spectrometry
Quantification is an essential task in comprehensive lipidomics studies challenged by the high number of lipids, their chemical diversity and their dynamic range (up to 7 orders of magnitude) of the lipidome.
Time-resolved and temperature tuneable measurements of fluorescent intensity using a smartphone fluorimeter
A smartphone fluorimeter is demonstrated for steady-state and time-resolved fluorescence intensity measurements at tunable temperatures.
In vitro field potential monitoring on a multi-microelectrode array for the electrophysiological long-term screening of neural stem cell maturation
Combining in vitro neuron cell models to automated electrophysiology monitoring allows the standardized identification of toxic and protective neuromodulators in pharmaceutical ingredient development.
Surfactant exfoliated 2D hexagonal Boron Nitride (2D-hBN) explored as a potential electrochemical sensor for dopamine: surfactants significantly influence sensor capabilities
The surfactant utilised in the exfoliated synthesis of 2D hexagonal Boron Nitride (2D-hBN) significantly influences sensor capabilities towards the detection of dopamine.
Implementation of MALDI MS profiling and imaging methods for the analysis of real crime scene fingermarks
First insights into MALDI MS based method's transition to operational casework involving fingermark analysis.
Simultaneous viscosity and density measurement of small volumes of liquids using a vibrating microcantilever
An analytical model is derived to calculate the viscosity and density of small volumes of fluid from the resonance frequencies of an immersed microcantilever. Its accuracy is verified on simple and non-Newtonian fluids.
Detection of chemical warfare agent simulants and hydrolysis products in biological samples by paper spray mass spectrometry
Paper spray ionization was used to detect and quantitate chemical warfare simulants and hydrolysis products from whole blood and urine.
Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation
Infrared spectral histopathology has shown great promise as an important diagnostic tool, with the potential to complement current pathological methods.
Specificity of SNP detection with molecular beacons is improved by stem and loop separation with spacers
Dissection of stem and loop regions in molecular beacons by nucleotide or non-nucleotide linkers minimizes nonspecific recognition in SNP discrimination.
Covalent immobilisation of antibodies in Teflon-FEP microfluidic devices for the sensitive quantification of clinically relevant protein biomarkers
Antibodies immobilised covalently within Teflon-FEP microcapillary devices allow sensitive fluorescent enzyme immunoassay.
Photochemistry in a soft-glass single-ring hollow-core photonic crystal fibre
A single-ring hollow-core photonic crystal fibre (HC-PCF), guided by anti-resonant reflection, is investigated as a highly efficient and versatile microreactor for liquid-phase photochemistry and catalysis.
Detection of glycosylation and iron-binding protein modifications using Raman spectroscopy
We have used Raman spectroscopy and chemometrics to determine protein modification as a result of glycosylation and iron binding.
Development of a full micro-scale spatially offset Raman spectroscopy prototype as a portable analytical tool
We present, for the first time, a portable full micro-Spatially Offset Raman Spectroscopy (micro-SORS) prototype permitting the in situ analysis of thin, highly turbid stratified layers at depths not accessible to conventional Raman microscopy.
Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy
Acute tissue biochemical response to traumatic brain injury is revealed using Raman spectroscopy.
Acoustofluidic particle trapping, manipulation, and release using dynamic-mode cantilever sensors
Active and programmable mixing, trapping, separation, manipulation and release of suspended particles in liquids using dynamic-mode cantilever sensors.
About this collection
This collection features recent open access articles published in Analyst.