Jump to main content
Jump to site search

Issue 9, 2019
Previous Article Next Article

Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model

Author affiliations

Abstract

Photodynamic therapy (PDT) involves a photosensitizing agent activated with light to induce cell death. Two-photon excited PDT (TPE-PDT) offers numerous benefits compared to traditional one-photon induced PDT, including an increased penetration depth and precision. However, the in vitro profiling and comparison of two-photon photosensitizers (PS) are still troublesome. Herein, we report the development of an in vitro screening platform of TPE-PS using a 3D osteosarcoma cell culture. The platform was tested using three different two-photon (2P) active compounds – a 2P sensitizer P2CK, a fluorescent dye Eosin Y, and a porphyrin derivative (TPP). Their 2P absorption cross-sections (σ2PA) were characterised using a fully automated z-scan setup. TPP exhibited a remarkably high σ2PA at 720 nm (8865 GM) and P2CK presented a high absorption at 850 nm (405 GM), while Eosin Y had the lowest 2P absorption at the studied wavelengths (<100 GM). The cellular uptake of PS visualized using confocal laser scanning microscopy showed that both TPP and P2CK were internalized by the cells, while Eosin Y stayed mainly in the surrounding media. The efficiency of the former two TPE-PS was quantified using the PrestoBlue metabolic assay, showing a significant reduction in cell viability after two-photon irradiation. The possibility of damage localization was demonstrated using a co-culture of adipose derived stem cells together with osteosarcoma spheroids showing no signs of damage to the surrounding healthy cells after TPE-PDT.

Graphical abstract: Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Jan 2019, accepted on 06 Mar 2019 and first published on 27 Mar 2019


Article type: Paper
DOI: 10.1039/C9AN00068B
Citation: Analyst, 2019,144, 3056-3063
  • Open access: Creative Commons BY license
  •   Request permissions

    Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model

    A. Dobos, W. Steiger, D. Theiner, P. Gruber, M. Lunzer, J. Van Hoorick, S. Van Vlierberghe and A. Ovsianikov, Analyst, 2019, 144, 3056
    DOI: 10.1039/C9AN00068B

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements