Jump to main content
Jump to site search

Issue 24, 2017

Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling

Author affiliations

Abstract

One of the promising approaches to address the challenge of detecting dilute nucleic acid analytes is exonuclease III-aided target recycling. In this strategy, the target DNA self-assembles with the reactant DNA probes and displays itself as a reactant and product at the same time. This provides an autonomous mechanism to release and reuse the analyte from each round of reactions for repetitive cycles, which amplifies the signal without amplifying the analyte itself. However, for very low amounts of the analyte, it takes a considerably long time before a detectable signal is generated. Thus, in this paper, we report a kinetically-enhanced target recycling strategy by designing two more target recycling sub-reactions that are triggered by the byproducts of the first reaction involving the target analyte. In this manner, concentrations of up to 0.5 pM of target DNA can be detected in 15 minutes.

Graphical abstract: Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling

Supplementary files

Article information


Submitted
28 Aug 2017
Accepted
05 Nov 2017
First published
06 Nov 2017

This article is Open Access

Analyst, 2017,142, 4782-4787
Article type
Paper

Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling

H. L. Lee Yu, Y. Zhang and I. Hsing, Analyst, 2017, 142, 4782 DOI: 10.1039/C7AN01423F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements