Low fouling strategies for electrochemical biosensors targeting disease biomarkers
This minireview summarizes recent trends in the development of low fouling electrochemical biosensors based on different antifouling materials.
Recent advances in single cell manipulation and biochemical analysis on microfluidics
This review highlights the advances in single cell manipulation and biochemical analysis on microfluidics over the past three years.
Integrated paper-based microfluidic devices for point-of-care testing
This review highlights the developments, accomplishments and challenges of integrated μPADs, including sample pretreatment, signal transduction/amplification and results output.
Integrated microfluidic systems with sample preparation and nucleic acid amplification
Integrated microfluidic systems with sample preparation and nucleic acid amplification can increase treatment efficiency and improve care.
Recent advances in nanomaterial-based electrochemical and optical sensing platforms for microRNA assays
This review describes recent efforts in the application of nanomaterials as sensing elements in electrochemical and optical miRNAs assays.
Molecular and living cell dynamic assays with optical microscopy imaging techniques
Compared with the conventional ensemble averaged measurements, single object analysis with optical microscopy can obtain the heterogeneous behavior of many individual objects, avoiding false judgment. Moreover, higher spatial and temporal resolution has been achieved by various optical imaging technologies.
Recent advances in single-cell analysis by mass spectrometry
Cells are the most basic structural units that play vital roles in the functioning of living organisms.
Advancing single-cell proteomics and metabolomics with microfluidic technologies
Recent advances in single-cell analysis have unraveled substantial heterogeneity among seemingly identical cells at genomic and transcriptomic levels.
Single-cell assay on microfluidic devices
Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations.
Boron-doped diamond: current progress and challenges in view of electroanalytical applications
This review examines the current status (from 2016 to December 2018) of the electroanalytical application of boron-doped diamond (BDD), in view of its advantages and challenges for electroanalytical applications.
Smartphone-based analytical biosensors
With the rapid development, mass production, and pervasive distribution of smartphones in recent years, they have provided people with portable, cost-effective, and easy-to-operate platforms to build analytical biosensors for point-of-care (POC) applications and mobile health.
Recent advances in microRNA detection
Recent advances in miRNA detection methods and new applications.
Graphene-based aptasensors: from molecule–interface interactions to sensor design and biomedical diagnostics
Graphene-based aptasensors exhibit wide applications for the biomedical detection of DNA, proteins, small molecules, viruses, bacteria, and cells.
Recent progress of particle migration in viscoelastic fluids
In this review, we discuss the up-to-date progress of particle migration in viscoelastic fluids mainly from the aspect of applications, laying out a comprehensive perspective on their potential in future lab-on-a-chip platforms.
Controllable synthesis of nanocrystals in droplet reactors
Different synthetic strategies derived from droplet reactors with integrated functional regions for NC synthesis.
Applications and developments of on-chip biochemical sensors based on optofluidic photonic crystal cavities
Photonic crystal (PC) cavities, which possess the advantages of compactness, flexible design, and suitability for integration in a lab-on-a-chip system, are able to distinguish slight variations in refractive index with only a small amount of analyte.
A novel alkaline phosphatase assay based on the specific chromogenic interaction between Fe3+ and ascorbic acid 2-phosphate
A new alkaline phosphatase sensing strategy based on the specific chromogenic interaction between ferric ion and ascorbic acid 2-phosphate was proposed.
A continuous-flow acoustofluidic cytometer for single-cell mechanotyping
A continuous-flow single-cell mechanotyping method which can decouple the cell size-dependent effect from the cell compressibility-dependent effect is presented.
Paper-based SERS analysis with smartphones as Raman spectral analyzers
We present a smartphone-based portable Raman spectrometer, which has a friendly human–machine interface, easy operation, rapid response time, and most importantly a very small size for on-site use.
A Ni3N–Co3N hybrid nanowire array electrode for high-performance nonenzymatic glucose detection
In this communication, we report a new type of nickel cobalt nitride hybrid nanowire array in situ grown on a Ti plate (Ni3N–Co3N NW/Ti) topotactically converted from Ni–Co hydroxide as a high-active catalyst electrode for glucose electrooxidation in alkaline media.
Electrocoalescence of liquid marbles driven by embedded electrodes for triggering bioreactions
We present coalescence of liquid marbles in a non-contact approach by applying electrostatics to embedded electrodes.
Microfluidic generation of cholesteric liquid crystal droplets with an integrative cavity for dual-gain and controllable lasing
We report cholesteric liquid crystal (CLC) triple-emulsion droplet lasers with controllable dual-gain and variable mode excitations, which offers a new route for optofluidic applications.
All-in-one microfluidic device for on-site diagnosis of pathogens based on an integrated continuous flow PCR and electrophoresis biochip
A miniaturized device based on integrated continuous flow PCR and electrophoresis microfluidic chip was developed for diagnosis of pathogens. It dispensed with costly external precision syringe pump and can realize rapid DNA amplification and on-site PCR products detection.
Controlled co-precipitation of biocompatible colorant-loaded nanoparticles by microfluidics for natural color drinks
Controlled co-precipitation of biocompatible colorant-loaded nanoparticles in microfluidic channels for natural color drinks.
A highly selective and sensitive red-emitting fluorescent probe for visualization of endogenous peroxynitrite in living cells and zebrafish
A highly selective and sensitive red-emitting fluorescent probe was constructed to visualize endogenous peroxynitrite in living cells and zebrafish.
Sensitive CVG-AFS/ICP-MS label-free nucleic acid and protein assays based on a selective cation exchange reaction and simple filtration separation
Sensitive CVG-AFS/ICP-MS label-free nucleic acid and protein assays based on a selective cation exchange reaction and simple filtration separation.
A NIR ratiometric fluorescent probe for the ‘naked-eye’ detection of endogenous hypochlorous acid in practical samples
We designed a near-infrared ratiometric (2.1 to 15.3, I608/I735) fluorescent probe with a quick response (20 s) based on cyanine polyene derivatives. This probe can detect endogenous hypochlorous acid in HepG-2 cells and determine the hypochlorous acid content in 84 disinfectant and other actual samples.
An electric-field-dependent drop selector
We have designed a superhydrophobic-coated chip to manipulate the static and dynamic behaviors of a drop using dielectrowetting.
Enzyme-triggered in situ formation of Ag nanoparticles with oxidase-mimicking activity for amplified detection of alkaline phosphatase activity
ALP-triggered in situ formation of Ag NPs with high oxidase-mimicking activity for colorimetric detection of alkaline phosphatase activity.
Engineering human islet organoids from iPSCs using an organ-on-chip platform
This work presents a new strategy to engineer functional human islet organoids from iPSCs in a perfusable organ-on-a-chip device.
A cascade toehold-mediated strand displacement strategy for label-free and sensitive non-enzymatic recycling amplification detection of the HIV-1 gene
A label-free biosensor was proposed for HIV-1 gene detection based on cascade toehold-mediated strand displacement reactions.
A novel electrochemical strategy based on porous 3D graphene-starch architecture and silver deposition for ultrasensitive detection of neuron-specific enolase
This work was aimed at designing a novel and ultrasensitive electrochemical immunoassay strategy to detect neuron-specific enolase (NSE) with a triple signal amplification strategy.
Dual role of BSA for synthesis of MnO2 nanoparticles and their mediated fluorescent turn-on probe for glutathione determination and cancer cell recognition
Scheme of the MnO2 NP-mediated fluorescence turn-off-on process of CDs with MnO2 NPs and GSH.
Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation
Herein we propose a label-free and sensitive detection method for coralyne and heparin, based on utilizing the complex of adenosine16 (A16) and coralyne to induce the formation of a G-quadruplex scaffold.
A liquid metal based capacitive soft pressure microsensor
A PDMS-based handy microsensor for on-chip microscale integrable pressure monitoring with high resolution.
Deep learning-based component identification for the Raman spectra of mixtures
DeepCID can achieve high accuracy, excellent sensitivity and few false positives for component identification in mixtures based on Raman spectroscopy and deep learning.
A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I
A multiple signal amplification of a SERS biosensor was developed for sensitive detection of cTnI with the aid of GO/AuNP complexes.
SERS-based droplet microfluidics for high-throughput gradient analysis
We report a fully integrated SERS-based microdroplet platform for simultaneous detection of various concentrations of a reagent.
A carbonothioate-based far-red fluorescent probe for the specific detection of mercury ions in living cells and zebrafish
A simple far-red fluorescent probe was developed to monitor mercury ions in environmental water samples and in living systems.
Rapid and simple detection of ascorbic acid and alkaline phosphatase via controlled generation of silver nanoparticles and selective recognition
A simple and novel strategy for detection of AA and ALP via controlled generation of Ag NPs and selective recognition.
Dual-template molecularly imprinted polymers for dispersive solid-phase extraction of fluoroquinolones in water samples coupled with high performance liquid chromatography
Dual-template molecularly imprinted polymers were synthesized using norfloxacin and enrofloxacin as templates by precipitation polymerization with a multi-template imprinting strategy.
Deposition of CdTe quantum dots on microfluidic paper chips for rapid fluorescence detection of pesticide 2,4-D
Novel fluorescent microfluidic paper chips were developed by the combination of molecularly imprinted polymers and microfluidic paper chips with CdTe quantum dots for the specific recognition and sensitive detection of the pesticide 2,4-D.
Rational design of a boron-dipyrromethene-based fluorescent probe for detecting Pd2+ sensitively and selectively in aqueous media
A novel fluorescent probe for Pd2+ based on the BODIPY fluorophore exploiting the PET (Photoinduced Electron Transfer) mechanism was designed and successfully synthesized.
Aluminum-doped NiO nanofibers as chemical sensors for selective and sensitive methanol detection
One-dimensional nanomaterials with controlled morphology have received considerable attention as chemical sensors.
A highly selective and instantaneously responsive Schiff base fluorescent sensor for the “turn-off” detection of iron(III), iron(II), and copper(II) ions
A new and tri-responsive fluorescent Schiff base probe (DBAB) has been designed and developed for the recognition of iron(III) ions (Fe3+), iron(II) ions (Fe2+) and copper(II) ions (Cu2+) simultaneously.
A microfluidic platform utilizing anchored water-in-oil-in-water double emulsions to create a niche for analyzing single non-adherent cells
Water-in-oil-in-water double emulsions (W/O/W DEs) are generated to encapsulate non-adherent cells and anchored in an array on-chip for in situ assays.
Double inner standard plot model of an electrophoresis titration chip for a portable and green assay of protein content in milk
High portability and environmental safety (“green”) are two of the most important objectives pursued by microfluidic methods.
Mesoporous Pd@Pt core–shell nanoparticles supported on multi-walled carbon nanotubes as a sensing platform: application in simultaneous electrochemical detection of anticancer drugs doxorubicin and dasatinib
A novel electrochemical sensor based on Pd@Pt/MWCNT composite for simultaneous detection of doxorubicin and dasatinib.
A mitochondria-targetable colorimetric and far-red fluorescent probe for the sensitive detection of carbon monoxide in living cells
A simple colorimetric and far-red fluorescent probe was developed for the first time to visualize the gasotransmitter carbon monoxide in subcellular mitochondria.
Cellular heterogeneity identified by single-cell alkaline phosphatase (ALP) via a SERRS-microfluidic droplet platform
We proposed an ultrasensitive method for studying low abundance ALP secreted by individual cell using the microfluidic droplet-based SERRS technique.
Flow stabilizer on a syringe tip for hand-powered microfluidic sample injection
We proposed a novel syringe flow-stabilizer for hand-powered, precise, continuous-flow microfluidic sample injection in resource-poor settings.
Facile fabrication of fluorescent Fe-doped carbon quantum dots for dopamine sensing and bioimaging application
A facile Fe-doped carbon quantum dot based fluorescent sensor for dopamine sensing and bioimaging was constructed.
Chitosan–gold nanocomposite and its functionalized paper strips for reversible visual sensing and removal of trace Hg2+ in practice
To eliminate mercury contamination in aqueous environment, chitosan–gold nanocomposite and its functionalized paper strips were designed and developed for visual sensing and removal of trace Hg2+.
A two-photon ratiometric fluorescent probe for highly selective sensing of mitochondrial cysteine in live cells
We report a novel two-photon ratiometric fluorescent probe (DNEPI) for highly selective sensing mitochondrial Cys in live cells.
Two aggregation-induced emission (AIE)-active reaction-type probes: for real-time detecting and imaging of superoxide anions
Two AIE-active reaction-type probes: one probe, two channels, at the same & different cell locations for superoxide anion.
A conjugated carbon-dot–tyrosinase bioprobe for highly selective and sensitive detection of dopamine
A conjugated carbon-dot–tyrosinase bioprobe was first designed and applied for sensitive and selective dopamine detection in human serum.
Porous graphene-coated stainless-steel fiber for direct immersion solid-phase microextraction of polycyclic aromatic hydrocarbons
Porous graphene was prepared by combustion method and fabricated as a new coating on stainless-steel fiber for direct immersion solid-phase microextraction of PAHs in environmental water.
Amino-functionalized mesoporous silica nanospheres (MSN-NH2) as sorbent for extraction and concentration of synthetic dyes from foodstuffs prior to HPLC analysis
MSN-NH2 was used as sorbent of dSPE for extraction and preconcentration of dyes from different foodstuffs prior to HPLC analysis.
Geographical traceability of cultivated Paris polyphylla var. yunnanensis using ATR-FTMIR spectroscopy with three mathematical algorithms
Discrimination ability for geographical origins of 3rd to 8th cultivation years Paris yunnanensis of PLS-DA was better than RF model.
Detection of microplastics in local marine organisms using a multi-technology system
Microplastics with complex polymer compositions are present in a lot of local marine organisms.
A high-throughput liquid biopsy for rapid rare cell separation from large-volume samples
The developed high-throughput liquid biopsy platform for rare tumor cell separation from body fluids has shown enormous promise in cancer detection and prognosis monitoring.
Rapid and selective DNA-based detection of melamine using α-hemolysin nanopores
We have developed a rapid and selective approach for the detection of melamine based on simple DNA probes and α-hemolysin nanopores.
Human brain organoid-on-a-chip to model prenatal nicotine exposure
We present a new strategy to generate stem cell based human brain organoids using an organ-on-a-chip system that allows us to model prenatal nicotine exposure.
Nanofiber membrane supported lung-on-a-chip microdevice for anti-cancer drug testing
A PLGA nanofiber membrane supported lung-on-a-chip microdevice was developed to model the alveolar microenvironment for anti-cancer drug testing.
About this collection
In celebration of Chinese New Year, Analyst, Analytical Methods and Lab on a Chip are delighted to present a special collection of articles by authors in China. This collection of articles highlights important analytical, bioanalytical and miniaturisation technologies and applications. Wishing everyone a very Happy Chinese New Year!