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Advancing single-cell proteomics and metabolomics with microfluidic technologies

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Abstract

Recent advances in single-cell analysis have unraveled substantial heterogeneity among seemingly identical cells at genomic and transcriptomic levels. These discoveries have urged scientists to develop new tools that are capable of investigating single cells from a broader set of “omics”. Proteomics and metabolomics, for instance, are of particular interest as they are closely correlated with a dynamic picture of cellular behaviors and phenotypic identities. The development of such tools requires highly efficient isolation and processing of a large number of individual cells, where techniques such as microfluidics are extremely useful. Here, we review the recent advances in single-cell proteomics and metabolomics, with a focus on microfluidics-based platforms. We highlight a vast array of emerging microfluidic formats for single-cell isolation and manipulation, and how the state-of-the-art analytical tools are coupled with such platforms for proteomic and metabolomic profiling.

Graphical abstract: Advancing single-cell proteomics and metabolomics with microfluidic technologies

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Publication details

The article was received on 05 Aug 2018, accepted on 29 Sep 2018 and first published on 01 Oct 2018


Article type: Critical Review
DOI: 10.1039/C8AN01503A
Citation: Analyst, 2019, Advance Article
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    Advancing single-cell proteomics and metabolomics with microfluidic technologies

    Y. Liu, X. Chen, Y. Zhang and J. Liu, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN01503A

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