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Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation

Abstract

Herein we propose a label-free and sensitive detection method for coralyne and heparin, based on utilizing the complex of 16-repeated adenosine (A16) and coralyne to induce the formation of a G-quadruplex scaffold. In the present work, two specific oligonucleotide strands were designed. Upon addition of coralyne, two oligonucleotide strands formed a double chain through A2-coralyne-A2 coordination, which induced the formation of the G-quadruplex accompanied by the proximity of two G-rich sequences. An increase in system fluorescence was observed after addition of N-methylmorpholine (NMM). In the presence of heparin, the formation of the heparin-coralyne complex caused coralyne to be removed from the A16-coralyne complex, leading to a weaker fluorescence output. At optimal concentrations, a linear coralyne response could be observed from 0.01-5 μM and the detection limit was estimated to be 5.8 nM. The detection limit of the heparin probe was as low as 1 nM, with a linear range from 1-100 nM. Overall, this study offers a sensitive and practical assay for coralyne and heparin detection.

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Supplementary files

Publication details

The article was accepted on 05 Feb 2019 and first published on 06 Feb 2019


Article type: Paper
DOI: 10.1039/C9AY00175A
Citation: Anal. Methods, 2019, Accepted Manuscript

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    Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation

    M. Chen, C. Ma, H. Zhao and K. Wang, Anal. Methods, 2019, Accepted Manuscript , DOI: 10.1039/C9AY00175A

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