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Themed collection Next wave advances in single cell analyses

26 items
Editorial

Next wave advances in single-cell analyses

Welcome to this Analyst themed issue highlighting next wave advances in single cell analyses, Guest Edited by Amy Herr (University of California, Berkeley, USA) Takehiko Kitamori (University of Tokyo, Japan), Ulf Landegren (Uppsala University, Sweden) and Masood Kamali-Moghaddam (Uppsala University, Sweden).

Graphical abstract: Next wave advances in single-cell analyses
From the themed collection: Next wave advances in single cell analyses
Minireview

New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells

We provide a vision for integrating pioneering mass spectrometry technologies for future applications in single cell analysis.

Graphical abstract: New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells
From the themed collection: Next wave advances in single cell analyses
Open Access Minireview

Advances in mass spectrometry based single-cell metabolomics

Single cell metabolomics using mass spectrometry can contribute to understanding biological activities in health and disease.

Graphical abstract: Advances in mass spectrometry based single-cell metabolomics
From the themed collection: Next wave advances in single cell analyses
Minireview

Quantitative imaging of lipid droplets in single cells

Non-destructive spatial characterization of lipid droplets using coherent Raman scattering microscopy and computational image analysis algorithms at the single-cell level.

Graphical abstract: Quantitative imaging of lipid droplets in single cells
From the themed collection: Next wave advances in single cell analyses
Minireview

Analysing single live cells by scanning electrochemical microscopy

Scanning electrochemical microscopy (SECM) offers single live cell activities along its topography toward cellular physiology and pathology.

Graphical abstract: Analysing single live cells by scanning electrochemical microscopy
From the themed collection: Next wave advances in single cell analyses
Minireview

Recent advances in single cell manipulation and biochemical analysis on microfluidics

This review highlights the advances in single cell manipulation and biochemical analysis on microfluidics over the past three years.

Graphical abstract: Recent advances in single cell manipulation and biochemical analysis on microfluidics
From the themed collection: Next wave advances in single cell analyses
Critical Review

High throughput screening of complex biological samples with mass spectrometry – from bulk measurements to single cell analysis

We review the state of the art in HTS using mass spectrometry with minimal sample preparation from complex biological matrices. We focus on industrial and biotechnological applications.

Graphical abstract: High throughput screening of complex biological samples with mass spectrometry – from bulk measurements to single cell analysis
From the themed collection: Next wave advances in single cell analyses
Critical Review

Molecular and living cell dynamic assays with optical microscopy imaging techniques

Compared with the conventional ensemble averaged measurements, single object analysis with optical microscopy can obtain the heterogeneous behavior of many individual objects, avoiding false judgment. Moreover, higher spatial and temporal resolution has been achieved by various optical imaging technologies.

Graphical abstract: Molecular and living cell dynamic assays with optical microscopy imaging techniques
From the themed collection: Next wave advances in single cell analyses
Critical Review

Advancing single-cell proteomics and metabolomics with microfluidic technologies

Recent advances in single-cell analysis have unraveled substantial heterogeneity among seemingly identical cells at genomic and transcriptomic levels.

Graphical abstract: Advancing single-cell proteomics and metabolomics with microfluidic technologies
From the themed collection: Next wave advances in single cell analyses
Critical Review

Recent advances in single-cell analysis by mass spectrometry

Cells are the most basic structural units that play vital roles in the functioning of living organisms.

Graphical abstract: Recent advances in single-cell analysis by mass spectrometry
From the themed collection: Next wave advances in single cell analyses
Critical Review

Single-cell assay on microfluidic devices

Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations.

Graphical abstract: Single-cell assay on microfluidic devices
From the themed collection: Next wave advances in single cell analyses
Paper

Crossing constriction channel-based microfluidic cytometry capable of electrically phenotyping large populations of single cells

This paper presents a crossing constriction channel-based microfluidic system for high-throughput characterization of specific membrane capacitance (Csm) and cytoplasm conductivity (σcy) of single cells.

Graphical abstract: Crossing constriction channel-based microfluidic cytometry capable of electrically phenotyping large populations of single cells
From the themed collection: Next wave advances in single cell analyses
Paper

Single cell analysis of aged RBCs: quantitative analysis of the aged cells and byproducts

This study focuses on characterizing the aging process of red blood cells by correlating the loss of hemoglobin and the translocation of phosphatidylserine (PS) in expired human red blood cells, hRBCs.

Graphical abstract: Single cell analysis of aged RBCs: quantitative analysis of the aged cells and byproducts
From the themed collection: Next wave advances in single cell analyses
Paper

Surface-enhanced Raman spectroscopy of microorganisms: limitations and applicability on the single-cell level

Detection and characterization of microorganisms is essential for both clinical diagnostics and environmental studies.

Graphical abstract: Surface-enhanced Raman spectroscopy of microorganisms: limitations and applicability on the single-cell level
From the themed collection: Next wave advances in single cell analyses
Paper

Dual cationic–anionic profiling of metabolites in a single identified cell in a live Xenopus laevis embryo by microprobe CE-ESI-MS

In situ capillary microsampling with capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry (MS) enabled the characterization of cationic and anionic metabolites in single cells in complex tissues and organisms, such as the live frog (X. laevis) embryo.

Graphical abstract: Dual cationic–anionic profiling of metabolites in a single identified cell in a live Xenopus laevis embryo by microprobe CE-ESI-MS
From the themed collection: Next wave advances in single cell analyses
Paper

Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose

With lethal opportunistic fungal infections on the rise, it is imperative to explore new methods to examine virulence mechanisms.

Graphical abstract: Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose
From the themed collection: Next wave advances in single cell analyses
Paper

Live single cell analysis using synchrotron FTIR microspectroscopy: development of a simple dynamic flow system for prolonged sample viability

A simple, cost-effective liquid sample holder to perform single live cell analysis under an IR microscope.

Graphical abstract: Live single cell analysis using synchrotron FTIR microspectroscopy: development of a simple dynamic flow system for prolonged sample viability
From the themed collection: Next wave advances in single cell analyses
Paper

Single-molecule DNA visualization using AT-specific red and non-specific green DNA-binding fluorescent proteins

Two-color DNA physical map for efficient identification of single DNA molecules.

Graphical abstract: Single-molecule DNA visualization using AT-specific red and non-specific green DNA-binding fluorescent proteins
From the themed collection: Next wave advances in single cell analyses
Paper

Gel-based cell manipulation method for isolation and genotyping of single-adherent cells

The simple and rapid method for isolation of single-adherent cells from a culture dish was developed and applied to genetic analysis of single-cells.

Graphical abstract: Gel-based cell manipulation method for isolation and genotyping of single-adherent cells
From the themed collection: Next wave advances in single cell analyses
Paper

Pushing the limits of detection for proteins secreted from single cells using quantum dots

Single cell secretion studies have provided valuable insight into population heterogeneity, but low detection sensitivity requires cells to secrete thousands of molecules. We employed semiconductor quantum dots and a new single particle imaging approach to improve detection sensitivity to only a few secreted molecules per cell.

Graphical abstract: Pushing the limits of detection for proteins secreted from single cells using quantum dots
From the themed collection: Next wave advances in single cell analyses
Paper

Single-cell mobility shift electrophoresis reports protein localization to the cell membrane

Prepending surface receptor immunostaining with single-cell polyacrylamide gel electrophoresis provides a new tool with which to understand how localization of surface receptor proteins controls the complex regulatory systems in single cells.

Graphical abstract: Single-cell mobility shift electrophoresis reports protein localization to the cell membrane
From the themed collection: Next wave advances in single cell analyses
Paper

Raman spectroscopy as a tool for tracking cyclopropane fatty acids in genetically engineered Saccharomyces cerevisiae

We demonstrate the first spectrum of cyclopropane fatty acid and track its presence in yeast using Raman spectroscopy and PLS-DA.

Graphical abstract: Raman spectroscopy as a tool for tracking cyclopropane fatty acids in genetically engineered Saccharomyces cerevisiae
From the themed collection: Next wave advances in single cell analyses
Paper

“Fix and assay”: separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative

Fixation of fluorescent sphingolipid-loaded cells enables cell metabolism and assay readout to be separated in time and space.

Graphical abstract: “Fix and assay”: separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative
From the themed collection: Next wave advances in single cell analyses
Paper

A new approach to find biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) by single-cell Raman micro-spectroscopy

Single-cell Raman microspectroscopy to detect phenylalanine as a potential biomarker for mitochondrial dysfunction and chronic fatigue syndrome.

Graphical abstract: A new approach to find biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) by single-cell Raman micro-spectroscopy
From the themed collection: Next wave advances in single cell analyses
Paper

Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry

Electrosyringe-assisted electrospray mass spectrometry (MS) is established for the first time to achieve intracellular sampling from one axon or dendrite in living neurons for mass spectrometric analysis.

Graphical abstract: Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry
From the themed collection: Next wave advances in single cell analyses
Paper

An integrated strategy for highly sensitive phosphoproteome analysis from low micrograms of protein samples

Phospho-SISPROT achieves highly sensitive phosphoproteome analysis from lower than 20 μg of cell lysates.

Graphical abstract: An integrated strategy for highly sensitive phosphoproteome analysis from low micrograms of protein samples
From the themed collection: Next wave advances in single cell analyses
26 items

About this collection

A collection of papers and reviews on next wave advances in single cell analyses focussing on emerging analytical advances in metabolomic, lipidomic, proteomic, and glycomic approaches – as well as high-dimensional approaches that unify multiple aspects of single-cell biology and medicine. As single-cell genomics and transcriptomics have already received wide attention, we aim to highlight the next generation of important analytical approaches and implications. This collection has been put together by guest leaders Takehiko Kitamori, Amy Herr, Ulf Landegren and Masood Kamali-Moghadamm.

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