Next wave advances in single-cell analyses
Welcome to this Analyst themed issue highlighting next wave advances in single cell analyses, Guest Edited by Amy Herr (University of California, Berkeley, USA) Takehiko Kitamori (University of Tokyo, Japan), Ulf Landegren (Uppsala University, Sweden) and Masood Kamali-Moghaddam (Uppsala University, Sweden).
Single cell transcriptomics: moving towards multi-omics
Single-cell multi-omics analysis helps characterize multiple layers of molecular features at a single-cell scale to provide insights into cellular processes and functions.
New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells
We provide a vision for integrating pioneering mass spectrometry technologies for future applications in single cell analysis.
Advances in mass spectrometry based single-cell metabolomics
Single cell metabolomics using mass spectrometry can contribute to understanding biological activities in health and disease.
Quantitative imaging of lipid droplets in single cells
Non-destructive spatial characterization of lipid droplets using coherent Raman scattering microscopy and computational image analysis algorithms at the single-cell level.
Analysing single live cells by scanning electrochemical microscopy
Scanning electrochemical microscopy (SECM) offers single live cell activities along its topography toward cellular physiology and pathology.
Recent advances in single cell manipulation and biochemical analysis on microfluidics
This review highlights the advances in single cell manipulation and biochemical analysis on microfluidics over the past three years.
High throughput screening of complex biological samples with mass spectrometry – from bulk measurements to single cell analysis
We review the state of the art in HTS using mass spectrometry with minimal sample preparation from complex biological matrices. We focus on industrial and biotechnological applications.
Molecular and living cell dynamic assays with optical microscopy imaging techniques
Compared with the conventional ensemble averaged measurements, single object analysis with optical microscopy can obtain the heterogeneous behavior of many individual objects, avoiding false judgment. Moreover, higher spatial and temporal resolution has been achieved by various optical imaging technologies.
Recent advances in single-cell analysis by mass spectrometry
Cells are the most basic structural units that play vital roles in the functioning of living organisms.
Advancing single-cell proteomics and metabolomics with microfluidic technologies
Recent advances in single-cell analysis have unraveled substantial heterogeneity among seemingly identical cells at genomic and transcriptomic levels.
Single-cell assay on microfluidic devices
Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations.
Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells
Coupling synchrotron IR beam to an ATR element enhances spatial resolution suited for high-resolution single cell analysis in biology, medicine and environmental science.
Crossing constriction channel-based microfluidic cytometry capable of electrically phenotyping large populations of single cells
This paper presents a crossing constriction channel-based microfluidic system for high-throughput characterization of specific membrane capacitance (Csm) and cytoplasm conductivity (σcy) of single cells.
Surface-enhanced Raman spectroscopy of microorganisms: limitations and applicability on the single-cell level
Detection and characterization of microorganisms is essential for both clinical diagnostics and environmental studies.
Single cell analysis of aged RBCs: quantitative analysis of the aged cells and byproducts
This study focuses on characterizing the aging process of red blood cells by correlating the loss of hemoglobin and the translocation of phosphatidylserine (PS) in expired human red blood cells, hRBCs.
Dual cationic–anionic profiling of metabolites in a single identified cell in a live Xenopus laevis embryo by microprobe CE-ESI-MS
In situ capillary microsampling with capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry (MS) enabled the characterization of cationic and anionic metabolites in single cells in complex tissues and organisms, such as the live frog (X. laevis) embryo.
Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose
With lethal opportunistic fungal infections on the rise, it is imperative to explore new methods to examine virulence mechanisms.
Live single cell analysis using synchrotron FTIR microspectroscopy: development of a simple dynamic flow system for prolonged sample viability
A simple, cost-effective liquid sample holder to perform single live cell analysis under an IR microscope.
Single-molecule DNA visualization using AT-specific red and non-specific green DNA-binding fluorescent proteins
Two-color DNA physical map for efficient identification of single DNA molecules.
Gel-based cell manipulation method for isolation and genotyping of single-adherent cells
The simple and rapid method for isolation of single-adherent cells from a culture dish was developed and applied to genetic analysis of single-cells.
Pushing the limits of detection for proteins secreted from single cells using quantum dots
Single cell secretion studies have provided valuable insight into population heterogeneity, but low detection sensitivity requires cells to secrete thousands of molecules. We employed semiconductor quantum dots and a new single particle imaging approach to improve detection sensitivity to only a few secreted molecules per cell.
Single-cell mobility shift electrophoresis reports protein localization to the cell membrane
Prepending surface receptor immunostaining with single-cell polyacrylamide gel electrophoresis provides a new tool with which to understand how localization of surface receptor proteins controls the complex regulatory systems in single cells.
Raman spectroscopy as a tool for tracking cyclopropane fatty acids in genetically engineered Saccharomyces cerevisiae
We demonstrate the first spectrum of cyclopropane fatty acid and track its presence in yeast using Raman spectroscopy and PLS-DA.
“Fix and assay”: separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative
Fixation of fluorescent sphingolipid-loaded cells enables cell metabolism and assay readout to be separated in time and space.
A new approach to find biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) by single-cell Raman micro-spectroscopy
Single-cell Raman microspectroscopy to detect phenylalanine as a potential biomarker for mitochondrial dysfunction and chronic fatigue syndrome.
Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry
Electrosyringe-assisted electrospray mass spectrometry (MS) is established for the first time to achieve intracellular sampling from one axon or dendrite in living neurons for mass spectrometric analysis.
An integrated strategy for highly sensitive phosphoproteome analysis from low micrograms of protein samples
Phospho-SISPROT achieves highly sensitive phosphoproteome analysis from lower than 20 μg of cell lysates.
About this collection
A collection of papers and reviews on next wave advances in single cell analyses focussing on emerging analytical advances in metabolomic, lipidomic, proteomic, and glycomic approaches – as well as high-dimensional approaches that unify multiple aspects of single-cell biology and medicine. As single-cell genomics and transcriptomics have already received wide attention, we aim to highlight the next generation of important analytical approaches and implications. This collection has been put together by guest leaders Takehiko Kitamori, Amy Herr, Ulf Landegren and Masood Kamali-Moghadamm.