Identifying the targets and functions of N-linked protein glycosylation in Campylobacter jejuni
Virulence of Campylobacter jejuni is dependent on the ability to glycosylate membrane-associated proteins.
Recombinant mucin-type proteins carrying LacdiNAc on different O-glycan core chains fail to support H. pylori binding
The β4-N-acetylgalactosaminyltransferase 3 (B4GALNT3) transfers GalNAc in a β1,4-linkage to GlcNAc forming the LacdiNAc (LDN) determinant on oligosaccharides.
β-Catenin/CBP inhibition alters epidermal growth factor receptor fucosylation status in oral squamous cell carcinoma
Genomic and structural analyses reveal that β-catenin/CBP signaling represses epidermal growth factor receptor (EGFR) N-glycan antennary fucosylation in oral cancer.
Choosing proper normalization is essential for discovery of sparse glycan biomarkers
In this work we assess the effect of different normalization methods on variable selection in an emerging field of glycomics.
Mass spectrometric analysis of core fucosylation and sequence variation in a human–camelid monoclonal antibody
ESI-MS fucosylation studies on an intact EG2-hFc monoclonal antibody reveal the presence of fucose on both Fc N-glycans.
Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis
The Xenopus laevis N-glycome undergoes massive reprogramming after the midblastula transition and the onset of neuronal development.
Rapid and sensitive glycan targeting by lectin-SERS assay
The fabrication of lectin-SERS nanotags and the assay designed for rapid glycoprotein identification and quantification.
Robustness in glycosylation systems: effect of modified monosaccharides, acceptor decoys and azido sugars on cellular nucleotide-sugar levels and pattern of N-linked glycosylation
Chemical perturbation studies reveal robustness in glycosylation systems, based on comparison of LC-MS/MS quantification of cellular nucleotide-sugar levels with the observed N-linked glycan patterns.
Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis
A high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis.
DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides
Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.
The effectiveness of filtering glycopeptide peak list files for Y ions
Novel software workflow for identifying additional glycopeptides in complex datasets.
Novel O-linked sialoglycan structures in human urinary glycoproteins
Identification of new glycoforms for glycopeptides confidently assigned from primary database searches permitting the most common O-glycans only.
Development of a 96-well plate sample preparation method for integrated N- and O-glycomics using porous graphitized carbon liquid chromatography-mass spectrometry
The developed workflow allows high throughput sample preparation for glycomics analysis.
Mycobacterium bovis BCG infection alters the macrophage N-glycome
Macrophage glycosylation that is essential to the host-immune defense may be modulated by pathogens infection.
Reduced sialyl-Lewisx on salivary MUC7 from patients with burning mouth syndrome
We analyse and compare MUC7 O-glycosylation and inflammatory biomarkers in saliva from female patients with burning mouth syndrome (BMS) and gender/age-matched controls.