Themed collection Analytical methods in chemical biology

29 items
Open Access Review Article

Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology

The integration of mass spectrometry with microfluidics has the potential to create high-throughput, sensitivity, and specificity assays.

Graphical abstract: Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

Alkyne functional groups have unique stretching frequency in the cell silent region. This review discusses the application of alkyne tags for Raman imaging in biological samples.

Graphical abstract: A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Imaging therapeutic peptide transport across intestinal barriers

Understanding how pharmaceutical peptides transport across the intestinal barrier could increase their bio-availability. To this end, fluorescence imaging offers a unique combination of spatiotemporal resolution and compatibility with living systems.

Graphical abstract: Imaging therapeutic peptide transport across intestinal barriers
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Recent developments and applications of quantitative proteomics strategies for high-throughput biomolecular analyses in cancer research

Various quantitative strategies can be utilized in mass spectrometry (MS)-based proteomic investigations. Here we explore recent applications of quantitative MS-based strategies and the resulting advances in the areas of prostate, pancreatic, breast and ovarian cancer research.

Graphical abstract: Recent developments and applications of quantitative proteomics strategies for high-throughput biomolecular analyses in cancer research
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Strategies to promote permeation and vectorization, and reduce cytotoxicity of metal complex luminophores for bioimaging and intracellular sensing

Metal complex luminophores have emerged as effective cell imaging contrast agents over the past decade. Here we review strategies employed to promote their cell permeability and sub-cellular targeting, critical challenges in imaging applications.

Graphical abstract: Strategies to promote permeation and vectorization, and reduce cytotoxicity of metal complex luminophores for bioimaging and intracellular sensing
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Optical chemosensors for the detection of proximally phosphorylated peptides and proteins

This review details optical detection methods for proximal multi-site phosphorylation, a critical post-translational modification in protein biology.

Graphical abstract: Optical chemosensors for the detection of proximally phosphorylated peptides and proteins
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Fluorescent proteins of the EosFP clade: intriguing marker tools with multiple photoactivation modes for advanced microscopy

In this review, we discuss structural and photophysical properties of photoactivatable EosFP and its engineered descendants and present a variety of applications in conventional and super-resolution fluorescence microscopy.

Graphical abstract: Fluorescent proteins of the EosFP clade: intriguing marker tools with multiple photoactivation modes for advanced microscopy
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Recent development of near-infrared photoacoustic probes based on small-molecule organic dye

In this review, we discuss the recent development of near-infrared photoacoustic probes based on small molecule dyes, which focus on their “always on” and “activatable” form in biomedicine.

Graphical abstract: Recent development of near-infrared photoacoustic probes based on small-molecule organic dye
From the themed collection: Articles in 2021
Open Access Review Article

Nanobodies as in vivo, non-invasive, imaging agents

In vivo imaging has become in recent years an incredible tool to study biological events and has found critical applications in diagnostic medicine.

Graphical abstract: Nanobodies as in vivo, non-invasive, imaging agents
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Advances in single-cell multi-omics profiling

Single-cell multi-omics profiling methods are developed to dissect heterogeneity of cell populations.

Graphical abstract: Advances in single-cell multi-omics profiling
From the themed collection: Analytical methods in chemical biology
Open Access Review Article

Labelling of DNA and RNA in the cellular environment by means of bioorthogonal cycloaddition chemistry

Labelling of nucleic acids as biologically important cellular components is a crucial prerequisite for the visualization and understanding of biological processes.

Graphical abstract: Labelling of DNA and RNA in the cellular environment by means of bioorthogonal cycloaddition chemistry
From the themed collection: Analytical methods in chemical biology
Open Access Communication

A silicon-rhodamine chemical-genetic hybrid for far red voltage imaging from defined neurons in brain slice

Small-molecule enzyme hybrids pair a far-red voltage-sensitive fluorophore with a cell-surface expressed HaloTag enzyme via a flexible linker to enable voltage imaging from genetically defined neurons in culture and brain slice.

Graphical abstract: A silicon-rhodamine chemical-genetic hybrid for far red voltage imaging from defined neurons in brain slice
From the themed collection: Analytical methods in chemical biology
Open Access Communication

Squaric acid as a new chemoselective moiety for mass spectrometry-based metabolomics analysis of amines

First application of squaric acids with excellent stability and chemoselective reactivity in mass spectrometry-based metabolomics analysis of amine-containing metabolites.

Graphical abstract: Squaric acid as a new chemoselective moiety for mass spectrometry-based metabolomics analysis of amines
From the themed collection: Analytical methods in chemical biology
Open Access Communication

Environment and coordination of FeMo–co in the nitrogenase metallochaperone NafY

Broadening of NMR resonance spins used to map binding of paramagnetic FeMo–co to the nitrogenase metallocluster escort protein NafY.

Graphical abstract: Environment and coordination of FeMo–co in the nitrogenase metallochaperone NafY
From the themed collection: Analytical methods in chemical biology
Open Access Communication

One-pot fluorescent assay for sensitive detection of APOBEC3A activity

A one-pot fluorescence-based assay to quantitively detect APOBEC3A activity was developed.

Graphical abstract: One-pot fluorescent assay for sensitive detection of APOBEC3A activity
From the themed collection: Analytical methods in chemical biology
Open Access Communication

An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells

Here, we report the development, computational modeling, in vitro enzymology, and biological application of an activity-based fluorescent sensor for the human phenol sulfotransferase SULT1A1.

Graphical abstract: An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
From the themed collection: Analytical methods in chemical biology
Open Access Communication

In vivo delivery of a fluorescent FPR2/ALX-targeted probe using focused ultrasound and microbubbles to image activated microglia

Targeted imaging agent labels activated microglia when delivered into the brain with focused ultrasound and microbubbles – a tool to investigate inflammation in neurological disorders.

Graphical abstract: In vivo delivery of a fluorescent FPR2/ALX-targeted probe using focused ultrasound and microbubbles to image activated microglia
From the themed collection: Analytical methods in chemical biology
Open Access Communication

Cellular internalization mechanism of novel Raman probes designed for plant cells

Diphenyl acetylene derivatives containing various polymeric components have been designed as new Raman imaging probes. These are taken up by plant cells via different pathways, and the internalization of exogenous molecules can be visualized.

Graphical abstract: Cellular internalization mechanism of novel Raman probes designed for plant cells
From the themed collection: Analytical methods in chemical biology
Open Access Communication

Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping

Chemical proteomics and cellular imaging reveal lysosomal trapping of tranylcypromine which can be largely reverted by the addition of lysosomotropic drugs.

Graphical abstract: Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping
From the themed collection: Analytical methods in chemical biology
Open Access Communication

Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis

The interface interactions of a type II peptidyl carrier protein and partner enzyme are observed to be unique and dynamic.

Graphical abstract: Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Ru(II)/BODIPY core co-encapsulated ratiometric nanotools for intracellular O2 sensing in live cancer cells

Uptake and quantitative ratiometric oxygen sensing response of core–shell nanoparticles containing ruthenium probe and BODIPY reference is demonstrated using a plate reader-based assay and by confocal xyλ scanning of live cancer cells under hypoxic conditions.

Graphical abstract: Ru(ii)/BODIPY core co-encapsulated ratiometric nanotools for intracellular O2 sensing in live cancer cells
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Versatile naphthalimide tetrazines for fluorogenic bioorthogonal labelling

New naphthalimide tetrazine probes permit fluorescent imaging of biomolecules in vitro and in living cells. They can be modified to provide previously unknown information about health and disease in biological systems.

Graphical abstract: Versatile naphthalimide tetrazines for fluorogenic bioorthogonal labelling
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Paper

Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes

A pair of orthogonal coiled coils templates highly specific live cell bioconjugation of two different proteins. PNA tagging and hybridisation with fluorophore–DNA reporters enables rapid dual receptor internalisation analysis of EGFR and ErbB2.

Graphical abstract: Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Click-based amplification: designed to facilitate various target labelling modes with ultralow background amplification

We describe a signal amplification method termed “Click-based amplification” that can be well integrated with various click-labelling modes, including chemical labelling, genetic incorporation and covalent inhibitor probe mediated target labelling.

Graphical abstract: Click-based amplification: designed to facilitate various target labelling modes with ultralow background amplification
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases

Modulation of real-time reversible nanoscale distribution of HCN2 channels using optogenetic activation of cAMP in live cells.

Graphical abstract: Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons

Voltage imaging with fluorescent indicators offers a powerful complement to traditional electrode or Ca2+-imaging approaches for monitoring electrical activity.

Graphical abstract: Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Fluorescent macrolide probes – synthesis and use in evaluation of bacterial resistance

Macrolide fluorescent probes illuminate the interactions between antibiotics and bacteria, providing new insight into mechanisms of resistance.

Graphical abstract: Fluorescent macrolide probes – synthesis and use in evaluation of bacterial resistance
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Combining bacteriophage engineering and linear dichroism spectroscopy to produce a DNA hybridisation assay

A novel DNA sensing method based on LD spectroscopy and using bionanoparticle scaffolds is described, as demonstrated by the rapid detection of DNA strands associated with bacterial and viral pathogens.

Graphical abstract: Combining bacteriophage engineering and linear dichroism spectroscopy to produce a DNA hybridisation assay
From the themed collection: Analytical methods in chemical biology
Open Access Paper

Biological evaluation of native streptococcal competence stimulating peptides reveals potential crosstalk between Streptococcus mitis and Streptococcus pneumoniae and a new scaffold for the development of S. pneumoniae quorum sensing modulators

Crosstalk between the Streptococcus mitis competence stimulating peptide (CSP) and the Streptococcus pneumoniae competence regulon quorum sensing circuitry was identified.

Graphical abstract: Biological evaluation of native streptococcal competence stimulating peptides reveals potential crosstalk between Streptococcus mitis and Streptococcus pneumoniae and a new scaffold for the development of S. pneumoniae quorum sensing modulators
From the themed collection: Analytical methods in chemical biology
29 items

About this collection

This collection seeks to highlight important advancements in fields such as imaging, spectroscopy, sensing and omics.  Analytical methods developed and utilized by chemical biologists encompass a wide range of techniques, including Raman probes for exploring uptake into plant cells, nanobodies for non-invasive imaging, single-cell multi-omics, single-molecule fluorescent sensors and much more.

This collection intends to highlight the importance of multidisciplinary research at the chemistry-biology interface.  Much of this exciting and ground-breaking research on the development and applications of analytical tools is collated here for you to explore.

New articles on the topic will be added periodically.

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