Issue 4, 2021
From the journal:

RSC Chemical Biology

One-pot fluorescent assay for sensitive detection of APOBEC3A activity†

Bingyao Wang,a   Xiong Zhang,a   Yafen Wang,a   Kun Chen, ORCID logo a   Fang Wang,b   Xiaocheng Weng ORCID logo *a  and  Xiang Zhou ORCID logo *a  

* Corresponding authors

a The Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, China
E-mail: xzhou@whu.edu.cn, xcweng@whu.edu.cn
Fax: +86-27-68756663
Tel: +86-27-68756663

b Wuhan University School of Pharmaceutical Sciences, Wuhan, China

Abstract

We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

Graphical abstract: One-pot fluorescent assay for sensitive detection of APOBEC3A activity

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Article information

DOI
https://doi.org/10.1039/D1CB00076D
Article type
Communication
Submitted
05 Apr 2021
Accepted
30 Apr 2021
First published
30 Apr 2021
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 1201-1205

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One-pot fluorescent assay for sensitive detection of APOBEC3A activity

B. Wang, X. Zhang, Y. Wang, K. Chen, F. Wang, X. Weng and X. Zhou, RSC Chem. Biol., 2021, 2, 1201 DOI: 10.1039/D1CB00076D

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