Personalised organs-on-chips: functional testing for precision medicine
Organs-on-chips can be ‘personalised’ so they can be used as functional tests to inform clinical decision-making for specific patients.
Maximizing the impact of microphysiological systems with in vitro–in vivo translation
The full impact of MPS technologies will be realized only when robust approaches for in vitro–in vivo (MPS-to-human) translation are developed and utilized.
Droplet microfluidics for the construction of compartmentalised model membranes
This tutorial review explores the use of droplet microfluidics to generate compartmentalised model membrane constructs that have potential applications as microreactors, as artificial cells in synthetic biology, as simplified cell models and as drug delivery vehicles.
Integration and application of optical chemical sensors in microbioreactors
This tutorial review explains the integration of optical chemical sensor formats for the purpose of monitoring oxygen, pH, carbon dioxide, glucose and temperature in microbioreactors.
Rotating magnetic particles for lab-on-chip applications – a comprehensive review
Magnetic particles are widely used in lab-on-chip and biosensing applications, because they have a high surface-to-volume ratio, they can be actuated with magnetic fields and many biofunctionalization options are available. This review focuses on the use of rotating magnetic particles for lab-on-chip applications.
Increasing the functionalities of 3D printed microchemical devices by single material, multimaterial, and print-pause-print 3D printing
3D printing has emerged as a valuable approach for the fabrication of fluidic devices and may replace soft-lithography as the method of choice for rapid prototyping.
Caring for cells in microsystems: principles and practices of cell-safe device design and operation
This review is geared towards device engineers, designers, and users who wish to establish “cell-friendly” technologies with utility to a broader scientific community.
Capillary microfluidics in microchannels: from microfluidic networks to capillaric circuits
This review critically analyzes the history, fundamental operating principles, limitations, applications, and emerging ideas related to microchannel-based capillary microfluidics.
Microsensor systems for cell metabolism – from 2D culture to organ-on-chip
We discuss microsensors for cell culture monitoring from 2D culture to organ-on-chip systems, including sensor principles, fabrication and culture formats.
Wearable sensors: modalities, challenges, and prospects
Non-invasive wearable sensing technology extracts mechanical, electrical, optical, and chemical information from the human body.
Recent advances in microfluidic technologies for cell-to-cell interaction studies
Microfluidic cell cultures are ideally positioned to become the next generation of in vitro diagnostic tools for biomedical research, where key biological processes such as cell signalling and dynamic cell-to-cell interactions can be reliably analysed under reproducible physiological cell culture conditions.
Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine
Breakthroughs in adapting microfluidic systems for exosome isolation, detection, and analysis are providing new tools to revolutionize personalized medicine.
Mobile microrobots for bioengineering applications
Untethered micron-scale mobile robots can navigate and non-invasively perform specific tasks inside unprecedented and hard-to-reach inner human body sites and inside enclosed organ-on-a-chip microfluidic devices with live cells.
The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck
Recent developments in the lab-on-PCB integration technology, highlighting its standardized industrial fabrication potential.
Advancements in microfluidics for nanoparticle separation
Nanoparticles have been widely implemented for healthcare and nanoscience industrial applications.
Waveguide-based chemo- and biosensors: complex emulsions for the detection of caffeine and proteins
Emulsion waveguides: a new modular sensing approach in which complex emulsions serve as efficient transducers in optical evanescent field-based waveguide sensors is reported.
Flow focusing through gels as a tool to generate 3D concentration profiles in hydrogel-filled microfluidic chips
We present a novel extension of laminar flow patterning using Darcy flow within cured three-dimensional hydrogels for precise delivery of solutes.
Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer
Biomolecular recognition can be attained by using the self-assembled monolayer modification for capture of a target analyte on the nanowire surfaces instead of primary antibodies.
A simple and low-cost chip bonding solution for high pressure, high temperature and biological applications
An adhesive-based strategy for the low-cost and reversible sealing of a wide range of materials used in microfluidics, requiring only the application of manually-achievable pressures.
On-chip integration of organic synthesis and HPLC/MS analysis for monitoring stereoselective transformations at the micro-scale
Seamless on-chip integration of a micro reaction unit and an HPLC functionality coupled to MS allows to study stereo selective chemical transformations at the microscale.
Microfluidic centrifugation assisted precipitation based DNA quantification
A microfluidic centrifugation assisted precipitation method for rapid DNA visualization and quantification.
High-throughput single-particle detections using a dual-height-channel-integrated pore
We report a proof-of-principle demonstration of particle concentration to achieve high-throughput resistive pulse detections of bacteria using a microfluidic-channel-integrated micropore.
Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli
We present a single-bacteria hydrodynamic trapping platform to detect antibiotic susceptibility and resistance by simultaneously monitoring motility and morphology of individual E. coli.
Overcoming transport limitations in miniaturized electrophoretic delivery devices
Combining experimental and theoretical approach, we demonstrate practical solutions to limiting currents in capillary-based electrophoretic delivery devices.
Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis
Implementing a new designed cooling system for rigorous temperature control and minimal acoustic energy losses allows high-throughput multiplex acoustophoresis.
Development of a multicellular pancreatic tumor microenvironment system using patient-derived tumor cells
The development of drugs to treat cancer is hampered by the inefficiency of translating pre-clinical in vitro monoculture and mouse studies into clinical benefit.
Array based real-time measurement of fluid viscosities and mass-densities to monitor biological filament formation
A membrane-based resonator detecting filament formation of biological samples in a label- and functionalization-free manner.
Grooved step emulsification systems optimize the throughput of passive generation of monodisperse emulsions
A novel step emulsifier allows high throughput production of monodisperse emulsions. Additionally, different modes of droplet production are investigated.
Inertial focusing with sub-micron resolution for separation of bacteria
Inertial focusing in curved channels is demonstrated for particles between 0.5 and 2.0 μm in diameter; a range of biological relevance since it comprises a multitude of bacteria and organelles of eukaryotic cells.
Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides
Lipid coated liquid crystal droplets have been trapped in a novel trap structure for the on-chip detection of a model antimicrobial peptide – Smp43, an α-helical peptide from Scorpion Venom.
Gradient in the electric field for particle position detection in microfluidic channels
In this work a new method to track particles in microfluidic channels is presented.
Formation of precisely composed cancer cell clusters using a cell assembly generator (CAGE) for studying paracrine signaling at single-cell resolution
Generation of exact cell clusters in the CAGE chip allows for paracrine signaling studies in models of specific tissue niches.
Modular soft robotic microdevices for dexterous biomanipulation
We present a methodology for building biologically inspired, soft microelectromechanical systems (MEMS) devices.
A microfluidic platform for the characterisation of membrane active antimicrobials
We quantify the membranolytic activity of antimicrobial peptides on biomimetic lipid vesicles in a multilayer microfluidic total analysis system.
Latchable microfluidic valve arrays based on shape memory polymer actuators
Latchable microfluidic valve arrays taking advantage of the shape memory polymer's multiple stable shapes and large change in stiffness for a small temperature variation.
Analysis and simulation of multiphase hydrodynamics in capillary microseparators
The capillary microseparator is an important microfluidic device for achieving the inline separation of biphasic segmented flows.
Ultra-high capacity microfluidic trapping of giant vesicles for high-throughput membrane studies
A high-capacity microfluidic platform designed to capture tens of thousands of giant vesicles for high-throughput membrane analysis.
Parahydrogen based NMR hyperpolarisation goes micro: an alveolus for small molecule chemosensing
We implemented continuous-flow SABRE NMR spectroscopy on a chip, unlocking low-volume chemosensing of strongly diluted analytes.
Acoustophoretic focusing effects on particle synthesis and clogging in microreactors
An acoustophoretic microreactor to manage particles in flow and to control the material synthesis process.
A lung/liver-on-a-chip platform for acute and chronic toxicity studies
A lung/liver-on-a-chip platform with metabolic capability over 28 days: a fit-for-purpose microfluidic system for toxicity assessment of pulmonary toxicants.
Correlative light and electron microscopy for complex cellular structures on PDMS substrates with coded micro-patterns
A correlative light and electron microscopy system that utilizes PDMS substrates with coded micro-patterns has been developed.
Microfluidic bead encapsulation above 20 kHz with triggered drop formation
Bead-triggered breakup of a fluid jet into monodispersed droplets improves the throughput of bead-based droplet workflows, enabling the analysis of large populations and the detection of rare events.
Screening of nutritional and genetic anemias using elastic light scattering
Elastic light scattering and machine learning accurately discriminates between healthy children, those with iron deficiency, and those with thalassemia minor.
An automated in vitro motility assay for high-throughput studies of molecular motors
Molecular motors, essential to force-generation and cargo transport within cells, are invaluable tools for powering nanobiotechnological lab-on-a-chip devices.
Millifluidic culture improves human midbrain organoid vitality and differentiation
The use of millifluidics technology for human midbrain organoid cultures reduces central cell death and increases dopaminergic neuron differentiation.
3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade
Microfluidic culture has the potential to revolutionize cancer diagnosis and therapy.
Effect of exercise on the plasma vesicular proteome: a methodological study comparing acoustic trapping and centrifugation
Improved reproducibility in seed particle mediated acoustic trapping of submicron particles enables clinical biomarker studies in extracellular vesicles.
Multi-parametric cell profiling with a CMOS quad-modality cellular interfacing array for label-free fully automated drug screening
A 1024-pixel CMOS quad-modality cellular interfacing array that enables multi-parametric cell profiling and holistic cellular characterization for drug development.
Quantitative phase microscopy of red blood cells during planar trapping and propulsion
Here, we have combined quantitative phase microscopy and waveguide trapping techniques to study changes in RBC morphology during planar trapping and transportation.
High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules
We developed micro-reactor arrays with a concentration gradient of target molecules for high-throughput single-molecule bioassay.
Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics
We introduce a new application of spiral inertial microfluidics for removing small nonviable cells from a mammalian suspension culture.
Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis
A novel technology for quantifying hormone secretion from tissues, with a single-cell resolution.
Acoustic formation of multicellular tumor spheroids enabling on-chip functional and structural imaging
Combining a protein repellent coating and ultrasonic standing waves in microwells enables on-chip formation and confocal microscopy of multicellular tumor spheroids.
Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays
This platform utilizes functionalized hydrogel posts contained within isolated nanoliter well reactors for quantitative and multiplex microRNA assays directly from unprocessed cell samples without needing prior nucleic acid extraction.
Sequencing of human genomes extracted from single cancer cells isolated in a valveless microfluidic device
Whole genome sequencing of single cancer cells isolated and lysed in an injection-moulded valveless microfluidic device.
A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide
Dual selection, high-resolution phenotypic screening and genotypic analysis of single-derived bacterial nanocultures using a 672-nanowell slide.
Enhanced ion transport using geometrically structured charge selective interfaces
A microfluidic platform containing charged hydrogels is used to investigate the effect of geometry on charge transport in electrodialysis applications.
A biomimetic microfluidic model to study signalling between endothelial and vascular smooth muscle cells under hemodynamic conditions
Cell signalling and mechanics influence vascular pathophysiology and there is an increasing demand for in vitro model systems that enable examination of signalling between vascular cells under hemodynamic conditions.
Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification
High-volume roll-to-roll manufacturing of PDMS–paper microfluidics.
Multiparameter cell-tracking intrinsic cytometry for single-cell characterization
We introduce a multiparameter intrinsic cytometry approach for single-cell characterization that combines ≥2 label-free measurement techniques onto the same platform.
Electrically controlled mass transport into microfluidic droplets from nanodroplet carriers with application in controlled nanoparticle flow synthesis
Infuse chemical into microdroplets from nanodroplet carriers with rates and dosages controlled by electrical fields, and applications in nanoparticle synthesis.
Inertial manipulation of bubbles in rectangular microfluidic channels
The position of bubbles in rectangular microchannels can be controlled by tuning the balance of forces acting on them.
Microfluidic label-free selection of mesenchymal stem cell subpopulation during culture expansion extends the chondrogenic potential in vitro
A microfluidic cell sorting based selective culture expansion method for mesenchymal stem cell improves cell yield and chondrogenic potential.
Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing
Precise fluid height sensing in open-channel microfluidics has long been a desirable feature for a wide range of applications.
Flocculation on a chip: a novel screening approach to determine floc growth rates and select flocculating agents
A novel microfluidic flocculation screening tool to efficiently compare flocculating agents and rapidly find the best flocculating condition using in situ detection of flocculation growth rates.
On-chip functional neuroimaging with mechanical stimulation in Caenorhabditis elegans larvae for studying development and neural circuits
New designs of microfluidic devices can facilitate recording of C. elegans larvae neuronal responses to precise mechanical stimuli, which reveal new understanding of development of mechanosensory neurons and circuits.
Acoustically modulated biomechanical stimulation for human cartilage tissue engineering
The biomechanical environment in an acoustofluidic bioreactor is modified by controlling the acoustic driving conditions to promote human cartilage generation.
A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement
Miniaturised conical measures for cell aggregates.
On-chip label-free protein analysis with downstream electrodes for direct removal of electrolysis products
Single-layer lithography microfluidic devices for applying high and stable electric fields on chip.
An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range
Fluorescence from four emission points is collimated by four lenses, split into four-color fluxes by four dichroic mirrors, and directly input into the image sensor.
About this collection
Please see below for recent Open Access papers published in Lab on a Chip.