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Issue 11, 2018

Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

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Abstract

Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS–paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

Graphical abstract: Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

Supplementary files

Article information


Submitted
15 Mar 2018
Accepted
19 Apr 2018
First published
30 Apr 2018

This article is Open Access

Lab Chip, 2018,18, 1552-1559
Article type
Paper

Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

J. Hiltunen, C. Liedert, M. Hiltunen, O. Huttunen, J. Hiitola-Keinänen, S. Aikio, M. Harjanne, M. Kurkinen, L. Hakalahti and L. P. Lee, Lab Chip, 2018, 18, 1552 DOI: 10.1039/C8LC00269J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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