Jump to main content
Jump to site search

Themed collection In memory of Chris Abell

61 items
Review Article

Coenzyme biosynthesis: enzyme mechanism, structure and inhibition

Key steps in vitamin biosynthesis: insights from mechanistic and structural studies.

Graphical abstract: Coenzyme biosynthesis: enzyme mechanism, structure and inhibition
From the themed collection: Vitamins and Cofactors
Review Article

Biosynthesis of pantothenate

A comprehensive review of recent developments in the enzymology of pantothenate biosynthesis, incorporating an historical perspective.

Graphical abstract: Biosynthesis of pantothenate
From the themed collection: In memory of Chris Abell
Tutorial Review

The use of FTICR-MS to detect chemical tags from a combinatorial library

From the themed collection: In memory of Chris Abell
Communication

Construction of core–shell microcapsules via focused surface acoustic wave microfluidics

A method to produce core–shell microcapsules with a solid core or microdroplet core via FSAW microfluidics is demonstrated.

Graphical abstract: Construction of core–shell microcapsules via focused surface acoustic wave microfluidics
From the themed collection: In memory of Chris Abell
Communication

Bioinspired hydrogel microfibres colour-encoded with colloidal crystals

The integration of microfluidic spinning and colloidal crystal microdots enable the colour-encoded hydrogel microfibres with facile and flexible manipulation of the encoding.

Graphical abstract: Bioinspired hydrogel microfibres colour-encoded with colloidal crystals
From the themed collection: In memory of Chris Abell
Open Access Communication

Cucurbit[7]uril-based high-performance catalytic microreactors

Fabrication of cucurbit[n]uril-based catalytic microreactors through the immobilisation of metallic nanoparticles onto microchannels.

Graphical abstract: Cucurbit[7]uril-based high-performance catalytic microreactors
From the themed collection: In memory of Chris Abell
Open Access Communication

Structural insights into the EthR–DNA interaction using native mass spectrometry

The interaction between Mycobacterium tuberculosis EthR and its operator DNA has been studied by native mass spectrometry, revealing an interesting stoichiometry.

Graphical abstract: Structural insights into the EthR–DNA interaction using native mass spectrometry
From the themed collection: In memory of Chris Abell
Open Access Communication

Surface-immobilised micelles via cucurbit[8]uril-rotaxanes for solvent-induced burst release

Surface-immobilised micelles via CB[8]-rotaxane host–guest recognition undergoes ‘burst’ release of cargo upon exposure to organic solvents.

Graphical abstract: Surface-immobilised micelles via cucurbit[8]uril-rotaxanes for solvent-induced burst release
From the themed collection: In memory of Chris Abell
Communication

Supramolecular colloidosomes: fabrication, characterisation and triggered release of cargo

Supramolecular colloidosomes are self-assembled at the interface of microfluidic droplets via a cucurbit[8]uril host–guest complex, allowing for triggered release of aqueous cargo.

Graphical abstract: Supramolecular colloidosomes: fabrication, characterisation and triggered release of cargo
From the themed collection: In memory of Chris Abell
Communication

Targeting a c-MYC G-quadruplex DNA with a fragment library

A fragment library was screened against the human c-MYC promoter G-quadruplex. Ten fragment hits had a significant concordance between a biophysical assay, in silico modelling and c-MYC expression inhibition.

Graphical abstract: Targeting a c-MYC G-quadruplex DNA with a fragment library
From the themed collection: In memory of Chris Abell
Communication

Surface-induced droplet fusion in microfluidic devices

Surface energy patterning in microfluidic channels provides a new tool for droplet fusion that does not require active elements nor accurate synchronization.

Graphical abstract: Surface-induced droplet fusion in microfluidic devices
From the themed collection: In memory of Chris Abell
Communication

Quantitative detection of protein expression in single cells using droplet microfluidics

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets. Using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size, fluorescence and cell occupancy.

Graphical abstract: Quantitative detection of protein expression in single cells using droplet microfluidics
From the themed collection: In memory of Chris Abell
Communication

Building three-dimensional nanostructures with active enzymes by surface templated layer-by-layer assembly

The first controlled fabrication of three-dimensional nanostructures with functional enzymes by templated layer-by-layer assembly of avidin and biotinylated horseradish peroxidase on micro-contact printing patterned surfaces is presented.

Graphical abstract: Building three-dimensional nanostructures with active enzymes by surface templated layer-by-layer assembly
From the themed collection: In memory of Chris Abell
Communication

Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA

Direct coupling of a dye-labelled DNA (acceptor) to a quantum dot (QD) donor significantly reduces the donor–acceptor distance and improves the FRET efficiency in quantum dot bioconjugates.

Graphical abstract: Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA
From the themed collection: In memory of Chris Abell
Communication

Rational design of new bifunctional inhibitors of type II dehydroquinase

New selective inhibitors of type II dehydroquinase were rationally designed to explore extra binding interactions, resulting in increased potency.

Graphical abstract: Rational design of new bifunctional inhibitors of type II dehydroquinase
From the themed collection: In memory of Chris Abell
Communication

Inhibition studies on salicylate synthase

Chorismate and isochorismate analogues were designed and tested as inhibitors of Yersinia enterocolitica salicylate synthase.

Graphical abstract: Inhibition studies on salicylate synthase
From the themed collection: In memory of Chris Abell
Communication

Enzymic synthesis of 3-[3-13C]dehydroquinic acid

3-[13C]Dehydroquinic acid has been prepared from commercially available D-5-[13C]fructose over four enzyme catalysed steps.

Graphical abstract: Enzymic synthesis of 3-[3-13C]dehydroquinic acid
From the themed collection: In memory of Chris Abell
Communication

Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites

The binding affinity of E. coli aspartate decarboxylase has been probed using MALDI-TOF spectrometry after incubation of the enzyme with a range of potential ligands in the presence of NaCNBH3. This has highighted key structural features which will aid design of potential inhibitors.

Graphical abstract: Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites
From the themed collection: In memory of Chris Abell
Communication

Systematic manipulation of surface chemical reaction on the nanoscale: a novel approach for constructing three-dimensional nanostructures

Nanoscale patches, created by nanografting a maleimide-terminated thiol into a self-assembled monolayer, were elaborated by sequential chemical reactions. Each state in the nanofabrication was followed by atomic force microscopy, providing a controlled approach to the fabrication of novel three-dimensional surface nanostructures.

Graphical abstract: Systematic manipulation of surface chemical reaction on the nanoscale: a novel approach for constructing three-dimensional nanostructures
From the themed collection: In memory of Chris Abell
Communication

Vinyl fluoride as an isoelectronic replacement for an enolate anion: Inhibition of type II dehydroquinases

From the themed collection: In memory of Chris Abell
Communication

Identification of Tyr58 as the proton donor in the aspartate-α-decarboxylase reaction

From the themed collection: In memory of Chris Abell
Open Access Edge Article

Aqueous interfacial gels assembled from small molecule supramolecular polymers

The successful self-assembly of a stimuli-responsive aqueous supramolecular hyperbranched polymer from small molecules and the macrocyclic host cucurbit[8]uril (CB[8]) is reported. This self-healing supramolecular network can act as a soft matter barrier at liquid–liquid interfaces.

Graphical abstract: Aqueous interfacial gels assembled from small molecule supramolecular polymers
From the themed collection: In memory of Chris Abell
Open Access Edge Article

Specific inhibition of CK2α from an anchor outside the active site

CAM4066, a specific CK2α kinase inhibitor, is anchored in the cryptic αD pocket outside the active site and inserts a “warhead” into the active site, blocking ATP binding and thereby inhibiting the kinase.

Graphical abstract: Specific inhibition of CK2α from an anchor outside the active site
From the themed collection: In memory of Chris Abell
Open Access Edge Article

Supramolecular hydrogel microcapsules via cucurbit[8]uril host–guest interactions with triggered and UV-controlled molecular permeability

Host–guest assembly at the interface of microfluidic droplets offers a versatile strategy to construct supramolecular hydrogel microcapsules with “smart” cargo release.

Graphical abstract: Supramolecular hydrogel microcapsules via cucurbit[8]uril host–guest interactions with triggered and UV-controlled molecular permeability
From the themed collection: In memory of Chris Abell
Edge Article

Fragment screening against the thiamine pyrophosphate riboswitchthiM

The development of a method to identify novel fragment ligands for the TPP riboswitch thiM is described.

Graphical abstract: Fragment screening against the thiamine pyrophosphate riboswitchthiM
From the themed collection: In memory of Chris Abell
Open Access Paper

Dual-responsive supramolecular colloidal microcapsules from cucurbit[8]uril molecular recognition in microfluidic droplets

Thermal and light dual-responsive supramolecular colloidal microcapsules have been successfully prepared by combining cucurbit[8]uril-based host–guest recognition with the self-assembly of colloidal particles within microfluidic droplets.

Graphical abstract: Dual-responsive supramolecular colloidal microcapsules from cucurbit[8]uril molecular recognition in microfluidic droplets
From the themed collection: In memory of Chris Abell
Open Access Paper

A fragment merging approach towards the development of small molecule inhibitors of Mycobacterium tuberculosis EthR for use as ethionamide boosters

With the ever-increasing instances of resistance to frontline TB drugs there is the need to develop novel strategies to fight the worldwide TB epidemic.

Graphical abstract: A fragment merging approach towards the development of small molecule inhibitors of Mycobacterium tuberculosis EthR for use as ethionamide boosters
From the themed collection: In memory of Chris Abell
Paper

Probing riboswitch–ligand interactions using thiamine pyrophosphate analogues

The binding of thiamine pyrophosphate (TPP) to the thiM riboswitch has been systematically explored by testing a range of TPP analogues in which the aminopyrimidine ring, the central thiazolium ring, and the pyrophosphate tail are each replaced in turn by similar structures.

Graphical abstract: Probing riboswitch–ligand interactions using thiamine pyrophosphate analogues
From the themed collection: In memory of Chris Abell
Paper

Controlling the contents of microdroplets by exploiting the permeability of PDMS

The protein crystallization and gene expression in vivo can be controlled by transporting small molecules through PDMS membrane to manipulate the chemical environment of microdroplets.

Graphical abstract: Controlling the contents of microdroplets by exploiting the permeability of PDMS
From the themed collection: In memory of Chris Abell
Paper

Microdroplet fabrication of silver–agarose nanocomposite beads for SERS optical accumulation

Highly uniform Ag–agarose microbeads prepared using a flow-focusing microfluidic device can trap and concentrate analytes increasing the analytical sensitivity of SERS.

Graphical abstract: Microdroplet fabrication of silver–agarose nanocomposite beads for SERS optical accumulation
From the themed collection: International Soft Matter Conference 2010
Paper

A chelating dendritic ligand capped quantum dot: preparation, surface passivation, bioconjugation and specific DNA detection

A chelating dendritic ligand capped stable, compact, water-soluble quantum dot allows for effective surface defect passivation and sensitive DNA detection.

Graphical abstract: A chelating dendritic ligand capped quantum dot: preparation, surface passivation, bioconjugation and specific DNA detection
From the themed collection: In memory of Chris Abell
Paper

Inhibition of chorismate-utilising enzymes by 2-amino-4-carboxypyridine and 4-carboxypyridone and 5-carboxypyridone analogues

A number of 2-amino-4-carboxypyridine, 4- and 5-carboxypyridone-based compounds were prepared. Several compounds proved to be low micromolar inhibitors when screened against three members of the chorismate-utilising enzyme family.

Graphical abstract: Inhibition of chorismate-utilising enzymes by 2-amino-4-carboxypyridine and 4-carboxypyridone and 5-carboxypyridone analogues
From the themed collection: In memory of Chris Abell
Paper

Hydrophilic PDMS microchannels for high-throughput formation of oil-in-water microdroplets and water-in-oil-in-water double emulsions

We developed an extremely convenient, largely automated surface modification technique for the production of long-term stable hydrophilic channel walls inside PDMS-based microfluidic devices and applied it successfully for the high-throughput generation of highly monodisperse o/w droplets and w/o/w double emulsions.

Graphical abstract: Hydrophilic PDMS microchannels for high-throughput formation of oil-in-water microdroplets and water-in-oil-in-water double emulsions
From the themed collection: In memory of Chris Abell
Paper

A double droplet trap system for studying mass transport across a droplet-droplet interface

Here we present the design, fabrication and operation of a microfluidic device to trap droplets in a large array of droplet pairs in a controlled manner.

Graphical abstract: A double droplet trap system for studying mass transport across a droplet-droplet interface
From the themed collection: In memory of Chris Abell
Paper

Vancomycin dimer formation between analogues of bacterial peptidoglycan surfaces probed by force spectroscopy

Force spectroscopy was used to investigate the rupture of interfacial vancomycin dimer complexes formed between pairs of vancomycin molecules when bound to model bacterial cell-wall surfaces.

Graphical abstract: Vancomycin dimer formation between analogues of bacterial peptidoglycan surfaces probed by force spectroscopy
From the themed collection: In memory of Chris Abell
Paper

Simultaneous measurement of reactions in microdroplets filled by concentration gradients

We demonstrate the integration of on-chip dilution with droplet formation, leading to parallel streams of microdroplets which allow the simultaneous measurements of enzymatic activity at different concentrations of substrate.

Graphical abstract: Simultaneous measurement of reactions in microdroplets filled by concentration gradients
From the themed collection: In memory of Chris Abell
Paper

An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets

A modular system of microfluidic PDMS devices was designed to incorporate the steps necessary for cell biological assays based on mammalian tissue culture ‘on-chip’. The design allows cultivation of cells for extended periods of time in combination with assessment of transfection rates and successive encapsulation of CHO-K1 cells achieved on-chip.

Graphical abstract: An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets
From the themed collection: In memory of Chris Abell
Paper

Synthesis and evaluation of 2,5-dihydrochorismate analogues as inhibitors of the chorismate-utilising enzymes

A library of 2,5-dihydrochorismate analogues were designed as inhibitors of the chorismate-utilising enzymes anthranilate synthase, isochorismate synthase, salicylate synthase and 4-amino-4-deoxychorismate synthase. The compounds, synthesised from shikimic acid, showed differential enzyme inhibition.

Graphical abstract: Synthesis and evaluation of 2,5-dihydrochorismate analogues as inhibitors of the chorismate-utilising enzymes
From the themed collection: In memory of Chris Abell
Paper

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

We describe the design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow.

Graphical abstract: Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays
From the themed collection: In memory of Chris Abell
Paper

The design and synthesis of inhibitors of pantothenate synthetase

Ten analogues of pantoyl adenylate, a reaction intermediate of the reaction catalysed by pantothenate synthetase, were synthesised as potential inhibitors of the enzyme. Analogues in which the phosphodiester was replaced by a sulfamoyl group were more potent than those in which it was replaced by an ester.

Graphical abstract: The design and synthesis of inhibitors of pantothenate synthetase
From the themed collection: In memory of Chris Abell
Paper

Design and synthesis of aromatic inhibitors of anthranilate synthase

Docking of aromatic chorismate analogue containing a C-4 hydroxymethyl substituent into the active site of Serratia marcescens anthranilate synthase.

Graphical abstract: Design and synthesis of aromatic inhibitors of anthranilate synthase
From the themed collection: In memory of Chris Abell
Paper

Design and synthesis of aromatic inhibitors of anthranilate synthase

Aromatic chorismate analogues were synthesised and tested against anthranilate synthase. The most potent compound exhibited a KI of 3 µM.

Graphical abstract: Design and synthesis of aromatic inhibitors of anthranilate synthase
From the themed collection: In memory of Chris Abell
Paper

(1R,4S,5R)-3-Fluoro-1,4,5-trihydroxy-2-cyclohexene-1-carboxylic acid: the fluoro analogue of the enolate intermediate in the reaction catalyzed by type II dehydroquinases

The fluoro analogue of the enolate intermediate in the reaction catalyzed by type II dehydroquinases has been prepared and has been shown to be the most potent inhibitor reported to date of the type II enzyme from Mycobacterium tuberculosis.

Graphical abstract: (1R,4S,5R)-3-Fluoro-1,4,5-trihydroxy-2-cyclohexene-1-carboxylic acid: the fluoro analogue of the enolate intermediate in the reaction catalyzed by type II dehydroquinases
From the themed collection: In memory of Chris Abell
Paper

A novel phase-switching protecting group for multi-step parallel solution phase synthesis

A novel protecting group that incorporates functionalities to facilitate compound purification by enabling solid-phase extraction in either an irreversible, or pH dependent reversible manner is presented, and its use in parallel synthesis is exemplified.

Graphical abstract: A novel phase-switching protecting group for multi-step parallel solution phase synthesis
From the themed collection: In memory of Chris Abell
Paper

Polymer-assisted solution phase synthesis of the antihyperglycemic agent Rosiglitazone (Avandia™)

The preparation of the antihyperglycemic agent Rosiglitazone (Avandia™) in high purity by a multi-step polymer-assisted solution phase synthesis requiring no conventional chromatography is reported.

Graphical abstract: Polymer-assisted solution phase synthesis of the antihyperglycemic agent Rosiglitazone (Avandia™)
From the themed collection: In memory of Chris Abell
Paper

Design, synthesis and evaluation of bifunctional inhibitors of type II dehydroquinase

The separate binding features of glycerol and a known inhibitor were combined in the design of a series of novel inhibitors of type II dehydroquinase.

Graphical abstract: Design, synthesis and evaluation of bifunctional inhibitors of type II dehydroquinase
From the themed collection: In memory of Chris Abell
Paper

A simple method for the preparation of 3-hydroxyiminodehydroquinate, a potent inhibitor of type II dehydroquinase

Graphical abstract: A simple method for the preparation of 3-hydroxyiminodehydroquinate, a potent inhibitor of type II dehydroquinase
From the themed collection: In memory of Chris Abell
Paper

Synthesis of 4-deoxyquinic, 4-deoxyshikimic and 4-deoxy-3-dehydroshikimic acids

From the themed collection: In memory of Chris Abell
Paper

Comparative study of the protection of modified and unmodified dsDNA by cationic and non-cationic lipids and liposomes to digestion by DNase I

From the themed collection: In memory of Chris Abell
Paper

Synthesis of (2R)-2-bromodehydroquinic acid and (2R)-2-fluorodehydroquinic acid

From the themed collection: In memory of Chris Abell
Paper

Synthesis of tetrahydroquinolines, hexahydrobenzoindolizines and an aryl phosphonate linker for the generation of catalytic antibodies

From the themed collection: In memory of Chris Abell
Paper

Comparison of the substrate specificity of type I and type II dehydroquinases with 5-deoxy- and 4,5-dideoxy-dehydroquinic acid

From the themed collection: In memory of Chris Abell
Paper

Design and synthesis of transition-state analogues for a cationic cyclisation

From the themed collection: In memory of Chris Abell
Paper

Different mechanistic and stereochemical courses for the reactions catalysed by type I and type II dehydroquinases

From the themed collection: In memory of Chris Abell
Paper

Studies on the biosynthesis of hydroxymellein using 17O n.m.r. and 2H n.m.r. spectroscopy to determine the origin of the C-4 hydroxy group

From the themed collection: In memory of Chris Abell
Paper

Biomimetic syntheses of the polyketide fungal metabolites alternariol and rubrofusarin: models for cyclisation reactions catalysed by polyketide synthase enzymes

From the themed collection: In memory of Chris Abell
Paper

Biosynthesis of 6-methylsalicylic acid; the combined use of mono- and tri-duteriated acetate precursors to investigate the degree of stereocontrol in the aromatisation sequence

From the themed collection: In memory of Chris Abell
Paper

Biosynthesis of the polyketide mellein from CD3CO2H and 13CD3CO2H in Aspergillus melleus: detection of deuterium by 2H n.m.r. and edited 13C n.m.r. spectra

From the themed collection: In memory of Chris Abell
Paper

Biosynthesis of the fungal metabolites alternariol, mellein, rubrofusarin, and 6-methylsalicylic acid from CD3CO2H

From the themed collection: In memory of Chris Abell
Paper

The use of 2H n.m.r. spectroscopy and β-isotopic shifts in the 13C n.m.r. spectrum to measure deuterium retention in the biosynthesis of the polyketide 6-methylsalicylic acid

From the themed collection: In memory of Chris Abell
Technical Innovation

Quantitative tracking of the growth of individual algal cells in microdroplet compartments

The work establishes the methodology to encapsulate, grow, and monitor single algal cells in microdroplets of aqueous medium suspended in fluorinated oil for extended periods of time.

Graphical abstract: Quantitative tracking of the growth of individual algal cells in microdroplet compartments
From the themed collection: In memory of Chris Abell
61 items

About this collection

Professor Chris Abell, who sadly passed away recently at the age of 62, was a pioneering interdisciplinary chemist, providing substantial contributions to the fields of fragment-based drug discovery and analytic advances in microfluidic microdroplets. 
Chris Abell has been very involved with the Royal Society of Chemistry in many capacities, including as an Editorial Board member for Organic & Biomolecular Chemistry and as an author on many journal articles. In this tribute to Chris Abell, we have collated a number of his publications across the journals Analyst, Chemical Communications, Chemical Science, Lab on a Chip, Materials Horizons, Nanoscale, Natural Product Reports, Organic & Biomolecular Chemistry, Polymer Chemistry and Soft Matter. The publications span his research career and we hope you enjoy revisiting some of his exceptional work.

Spotlight

Advertisements