Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies
How can microfluidics address the significant limitations to the current tools that measure gene expression in single-animal studies?
Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics
Tumor-on-a-chip platforms to study cancer-immune system crosstalk in the era of immunotherapy
Trapping and control of bubbles in various microfluidic applications
Active and passive techniques for bubble trapping and control in various microfluidic applications.
A facile multi-material direct laser writing strategy
We present an accessible strategy for printing multi-material 3D nanostructured components via microfluidic two-photon direct laser writing.
A miniaturized optical tomography platform for volumetric imaging of engineered living systems
Volumetric optical microscopy approaches that enable acquisition of three-dimensional (3D) information from a biological sample are attractive for numerous non-invasive imaging applications.
Microfluidics-enabled rational design of immunomagnetic nanomaterials and their shape effect on liquid biopsy
A microfluidics-enabled strategy for the controllable synthesis of immunomagnetic nanomaterials was developed, and the shape-dependent screening efficiency of CTCs was investigated.
An integrated microfluidic system for early detection of sepsis-inducing bacteria
Isolation and identification of bacteria from human blood were automated on an integrated microfluidic system for sepsis-inducing bacteria.
Dynamic pneumatic rail enabled microdroplet manipulation
A high-throughput multiplexed microfluidic device for COVID-19 serology assays
A high-throughput cell culture system based on capillary and centrifugal actions for rapid antimicrobial susceptibility testing
A capillary and centrifuge-based rapid antimicrobial susceptibility testing system is developed to reduce the time of loading the sample and culture media while achieving a high-throughput testing capacity.
Modular off-chip emulsion generator enabled by a revolving needle
An integrated revolving needle emulsion generator (RNEG) is developed to achieve high-throughput production of monodispersed droplets in an off-chip manner.
Everything in its right place: controlling the local composition of hydrogels using microfluidic traps
We introduce a microfluidic Hele-Shaw trapping cell that enables the fabrication of hydrogel sheets whose composition can be abruptly and controllably changed.
Soft elastomeric composite materials with skin-inspired mechanical properties for stretchable electronic circuits
An elastomeric composite material with skin-like mechanical responses and spatially heterogeneous rigidity is developed to accommodate stretchable electronic circuit systems.
Intelligent whole-blood imaging flow cytometry for simple, rapid, and cost-effective drug-susceptibility testing of leukemia
The drug susceptibility of leukemia cells in whole blood is evaluated by using extreme-throughput imaging flow cytometry with deep learning.
Clinical translation of microfluidic sensor devices: focus on calibration and analytical robustness
Modular platform providing the building blocks to translate specialised microfluidics from the laboratory to a clinical environment.
The biomolecular corona of gold nanoparticles in a controlled microfluidic environment
The use of microfluidic devices may allow standardizing corona formation protocols and comparing data from different laboratories.
A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting
A gold bowtie nanoantenna integrated with a 30 nm × 30 nm nanochannel for single particle detection and counting in real time.
Measurement of the magnetic susceptibility of subtle paramagnetic solutions using the diamagnetic repulsion of polymer microparticles
The diamagnetic repulsion of polymer microspheres in a microfluidic platform enables the discrimination of magnetic susceptibility of subtle paramagnetic solutions.
Remotely controlled nanofluidic implantable platform for tunable drug delivery
An implantable nanofluidic device for remote controlled drug delivery.
Two-dimensional planar swimming selects for high DNA integrity sperm
Selection of high DNA integrity sperm via planar swimming in a microfluidic device outperforms clinically established sperm preparation.
Controlled co-precipitation of biocompatible colorant-loaded nanoparticles by microfluidics for natural color drinks
Controlled co-precipitation of biocompatible colorant-loaded nanoparticles in microfluidic channels for natural color drinks.
Microfluidic blood vasculature replicas using backside lithography
A one-mask microfabrication process to build artificial vascular networks for the analysis of blood flows and hemophysics.
Rapid separation and identification of beer spoilage bacteria by inertial microfluidics and MALDI-TOF mass spectrometry
Microfluidics and MALDI-TOF MS is a rapid, high-throughput, and accurate method for the identification of beer spoilage bacteria.
Condensing-enriched magnetic photonic barcodes on superhydrophobic surface for ultrasensitive multiple detection
When PhC barcodes with stable characteristic reflection peaks are integrated with a superhydrophobic substrate they can realize the ultrasensitive multiple detection by the enrichment of the solution upon evaporation. Thus, the detection of miRNA was carried out by the system and it was found the detection limit was improved by about three orders.
Gel-on-a-chip: continuous, velocity-dependent DNA separation using nanoscale lateral displacement
We studied the dsDNA trajectories being advected while diffusing in a pressure driven flow in a nanoDLD array.
Traction microscopy with integrated microfluidics: responses of the multi-cellular island to gradients of HGF
Microfluidic system integrated with cell collectives and traction microscopy demonstrates that collective cell migration plays a central role in development, regeneration, and metastasis.
High-throughput single-particle detections using a dual-height-channel-integrated pore
We report a proof-of-principle demonstration of particle concentration to achieve high-throughput resistive pulse detections of bacteria using a microfluidic-channel-integrated micropore.
Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism
A new label-free and passive microfluidic technique to select cells based on single-cell glycolytic activity. The method has broad applicability and we demonstrate here single-cell droplet encapsulation and selection of live cells.
Tailoring surface acoustic wave atomisation for cryo-electron microscopy sample preparation
Surface acoustic wave (SAW) atomisation is investigated in the context of cryo electron microscopy grid preparation. Here, the primary requirements are a reproducible and narrow plume of droplets delivering a low fluid flow rate.
High-throughput mechanotransduction in Drosophila embryos with mesofluidics
A mesofluidic device allows alignment, immobilization, compression, imaging, and recovery of hundreds of live Drosophila embryos to investigate mechanotransduction.
On-chip stool liquefaction via acoustofluidics
An acoustofluidic chip can liquefy stool samples in a continuous flow.
Modular soft robotic microdevices for dexterous biomanipulation
We present a methodology for building biologically inspired, soft microelectromechanical systems (MEMS) devices.
Latchable microfluidic valve arrays based on shape memory polymer actuators
Latchable microfluidic valve arrays taking advantage of the shape memory polymer's multiple stable shapes and large change in stiffness for a small temperature variation.
Plastic-based acoustofluidic devices for high-throughput, biocompatible platelet separation
A plastic-based acoustofluidic device for high-throughput platelet separation consisting of a transducer and a disposable chip.
Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation
We report here a 96 well-plate based pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation.
Natural gas vaporization in a nanoscale throat connected model of shale: multi-scale, multi-component and multi-phase
Production of hydrocarbons from shale is a complex process that necessitates the extraction of multi-component hydrocarbons trapped in multi-scale nanopores.
Versatile platform for performing protocols on a chip utilizing surface acoustic wave (SAW) driven mixing
We present a dextrous microfluidic device which features a reaction chamber with volume flexibility and acoustic mixing.
A microfluidic co-cultivation platform to investigate microbial interactions at defined microenvironments
A microfluidic device for the co-cultivation of microbial cells.
Urine-based liquid biopsy: non-invasive and sensitive AR-V7 detection in urinary EVs from patients with prostate cancer
Detection of AR-V7 in urinary EVs provides a simple and promising liquid biopsy tool for patients with prostate cancer.
Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles
Post-synthesis functionalized hydrogel microparticles were demonstrated in multiplex immunoassays with high sensitivity, a wide assay range, and fast detection.
High-throughput, non-equilibrium studies of single biomolecules using glass-made nanofluidic devices
Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples.
Microfluidic bead encapsulation above 20 kHz with triggered drop formation
Bead-triggered breakup of a fluid jet into monodispersed droplets improves the throughput of bead-based droplet workflows, enabling the analysis of large populations and the detection of rare events.
Extraction of electrokinetically separated analytes with on-demand encapsulation
On-demand two-phase encapsulation of electrokinetically separated analytes decouples electrokinetic separations from downstream analytical processes.
Selective assembly and functionalization of miniaturized redox capacitor inside microdevices for microbial toxin and mammalian cell cytotoxicity analyses
We report a novel strategy for bridging information transfer between electronics and biological systems within microdevices.
Determining the lipid specificity of insoluble protein transmembrane domains
Overcoming solubility of membrane protein transmembrane domains to study their specificity to lipid compositions.
Novel devices for studying acute and chronic mechanical stress in retinal pigment epithelial cells
Replicating mechanical stress using two novel devices revealed that mechanical stress in retinal pigment epithelial cells induces choroidal neovascularization.
Measurement and mitigation of free convection in microfluidic gradient generators
Microfluidic gradient generators are used to study the movement of living cells, lipid vesicles, and colloidal particles in response to spatial variations in their local chemical environment.
Integrated measurement of intracellular proteins and transcripts in single cells
Single cell coupled proteomic and transcriptomic measurements are captured on a scalable microfluidic device.
3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade
Microfluidic culture has the potential to revolutionize cancer diagnosis and therapy.
A microfluidic approach to study the effect of mechanical stress on erythrocytes in sickle cell disease
This work presents a microfluidic device to challenge the deformability of normal and pathological red cells and their resistance to lysis upon mechanical stress.
Localized mechanical stimulation of single cells with engineered spatio-temporal profile
We introduce a new platform for mechanobiology based on active substrates, made of Fe-coated polymeric micropillars, capable to apply mechanical stimuli with tunable spatio-temporal profile on a cell culture.
Automated microfluidic droplet sampling with integrated, mix-and-read immunoassays to resolve endocrine tissue secretion dynamics
A fully automated droplet generation and analysis device based on pressure driven push-up valves for precise pumping of fluid and volumetric metering has been developed for high resolution hormone secretion sampling and measurement.
Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system
A novel microfluidic platform for extracellular vesicle extraction, microRNA isolation and detection with field-effect transistors for early detection of cardiovascular diseases.
Micro-droplet arrays for micro-compartmentalization using an air/water interface
Here we present a water-in-air droplet platform for micro-compartmentalization for single molecule guided synthesis and analysis consisting of a flow-system hosting dense arrays of aqueous microdroplets on a glass surface surrounded by air.
A system to monitor statin-induced myopathy in individual engineered skeletal muscle myobundles
Microphysiological tissue engineering models of human skeletal muscle (myobundles) in series with a calibrated elastic membrane provide a platform to investigate the mechanism of muscle diseases and to study the response to drugs and toxins in vitro.
Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells
Tissues are increasingly being analyzed at this single cell level. We present a simple and inexpensive microfluidic filter device that can rapidly and effectively improve the quality of single cell suspensions obtained from digested tissue samples.
UniChip enables long-term recirculating unidirectional perfusion with gravity-driven flow for microphysiological systems
UniChip enables recirculating unidirectional perfusion with gravity-driven flow, facilitating reliable and cost-effective integration of shear stress-sensitive tissues into microphysiological systems.
Engineering cell heterogeneity into organs-on-a-chip
To improve predictive efficacy of organ-on-a-chip devices, developers must consider cell heterogeneity.
Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays
This platform utilizes functionalized hydrogel posts contained within isolated nanoliter well reactors for quantitative and multiplex microRNA assays directly from unprocessed cell samples without needing prior nucleic acid extraction.
NemaFlex: a microfluidics-based technology for standardized measurement of muscular strength of C. elegans
NemaFlex measures a behavior- and gait-independent measure of C. elegans strength analogous to maximum voluntary force in humans.
Microstructure guided multi-scale liquid patterning on an open surface
A simple and fast multi-scale microliquid patterning on an open surface is demonstrated.
A multiplexed microfluidic system for evaluation of dynamics of immune–tumor interactions
A system for perfusing and interacting tumor fragments and immune cells and testing drug response with image analytics is reported.
Isolation and genome sequencing of individual circulating tumor cells using hydrogel encapsulation and laser capture microdissection
Single CTC sequencing workflow using biophysical enrichment and single cell isolation by laser capture microdissection.
Double-sided 3D printing on paper towards mass production of three-dimensional paper-based microfluidic analytical devices (3D-μPADs)
Recently, much effort has been focused on developing three-dimensional, paper-based microfluidic analytical devices (3D-μPADs) targeting in vitro diagnostics.
Multiparameter cell-tracking intrinsic cytometry for single-cell characterization
We introduce a multiparameter intrinsic cytometry approach for single-cell characterization that combines ≥2 label-free measurement techniques onto the same platform.
Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering
We present an injection molded lab-on-a-disc enabling quantification of bacterial metabolites in E. coli supernatant.
Engineering reaction–diffusion networks with properties of neural tissue
The application of soft lithography to create reaction–diffusion networks capable of generating spatiotemporal patterns analogous to biological central pattern generators.
About this collection
Here is a collection of recent Lab on a Chip articles which have been classified as ‘HOT articles’ due to receiving particularly high scores at peer review.