Jump to main content
Jump to site search

Issue 2, 2019
Previous Article Next Article

Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

Author affiliations

Abstract

We report here a novel pumpless, 96-well plate-based platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation. A gravity-driven flow strategy was developed to generate and sustain the flow rate of culture medium within 10% in the platform's 20 culture chambers. The ability of the platform to generate and sustain the medium flow was demonstrated by computational simulation, flow visualization, and ascertaining the previously known effect of flow-induced shear stress on the stimulated osteogenic differentiation of osteoblasts. The high-throughput utility of the platform was demonstrated by in situ cell staining and high content screening of chemosensitivity assays of multiple myeloma and osteoblast co-cultures. Endpoint characterization and data analyses for all 20 culture chambers required less than 1 hour.

Graphical abstract: Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

Back to tab navigation

Supplementary files

Article information


Submitted
21 Aug 2018
Accepted
06 Dec 2018
First published
11 Dec 2018

Lab Chip, 2019,19, 254-261
Article type
Paper

Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

Z. Chen, S. He, J. Zilberberg and W. Lee, Lab Chip, 2019, 19, 254
DOI: 10.1039/C8LC00872H

Social activity

Search articles by author

Spotlight

Advertisements