Themed collection Clinical spectroscopy
SPEC 2018: International Society of Clinical Spectroscopy
Welcome to the latest themed issue on the topic of Optical Diagnosis in Analyst, guest-edited by Dr. Matthew Baker.
Optical diagnosis – highlighting the clinical applications of vibrational spectroscopy
Welcome to the latest themed issue on the topic of Optical Diagnosis in Analyst, guest-edited by Kathleen Gough and Frédéric Leblond.
Biomedical applications of mid-infrared quantum cascade lasers – a review
Quantum cascade lasers with high spectral power density and narrow band emission profiles provide novel access to biomedical applications.
Clinical applications of infrared and Raman spectroscopy: state of play and future challenges
This review examines the state-of-the-art of clinical applications of infrared absorption and Raman spectroscopy, outstanding challenges, and progress towards translation.
Raman spectroscopy for cancer detection and cancer surgery guidance: translation to the clinics
This review summarizes actual clinical needs in oncology that can be addressed by Raman spectroscopy, provides results published in the last 10 years and analyses the current status of translation of these results into clinical practice.
Raman technologies in cancer diagnostics
This review explores Raman technologies with a particular emphasis on their utility for cancer diagnostic applications.
Can mid-infrared biomedical spectroscopy of cells, fluids and tissue aid improvements in cancer survival? A patient paradigm
This review takes a fresh approach from the patient perspective; offering insight into the applications of midinfrared biomedical spectroscopy.
Dual purpose fibre – SERS pH sensing and bacterial analysis
A way to incorporate SERS nanosensors on the end of an optical fibre that also allows for the extraction of bacterial samples.
Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell
First IR-SNOM images over the Amide I band taken in the centre of an oesophageal cancer cell.
Identification of bacterial species by untargeted NMR spectroscopy of the exo-metabolome
Identification of bacterial species is a crucial bottleneck for clinical diagnosis of infectious diseases.
Three-dimensional depth profiling of prostate tissue by micro ATR-FTIR spectroscopic imaging with variable angles of incidence
Variable angle micro ATR-FTIR, via the insertion of circular apertures, was used to measure tissue samples at various penetration depths.
Raman spectroscopy for the evaluation of the radiobiological sensitivity of normal human breast cells at different time points after irradiation by a clinical proton beam
Radiobiological effects occurring in normal human breast cells exposed to a low dose of a clinical proton beam are detectable by means of Raman spectra and the ratiometric analysis of Raman peak intensities.
Detection of leucine aminopeptidase activity in serum using surface-enhanced Raman spectroscopy
A specific reaction-based SERS approach was developed for the selective and sensitive detection of leucine aminopeptidase activity in serum.
In-line whole blood fractionation for Raman analysis of blood plasma
Raman studies of dynamically expanded cell-free plasma domains in microfluidic blood flow.
Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480
A novel photosensitiser, DC473, designed with solvatochromatic fluorescence and distinct Raman signal, is detected with tandem fluoRaman in SW480 cells.
Characterization of inflammatory breast cancer: a vibrational microspectroscopy and imaging approach at the cellular and tissue level
Inflammatory breast cancer (IBC) has a poor prognosis because of the lack of specific biomarkers and its late diagnosis.
Hyperspectral analysis applied to micro-Brillouin maps of amyloid-beta plaques in Alzheimer's disease brains
Non-negative factorization analysis applied to spontaneous Brillouin microscopy maps of amyloid-beta plaques in a transgenic mouse model enables to resolve spatially distinct components with specific mechanical properties.
Optimised spectral pre-processing for discrimination of biofluids via ATR-FTIR spectroscopy
Pre-processing is an essential step in the analysis of spectral data.
Exploring the efficacy and cellular uptake of sorafenib in colon cancer cells by Raman micro-spectroscopy
This study demonstrates the efficacy and distribution of sorafenib in colon cancer cells by label-free Raman microscopy.
Different binding sites of serum albumins in the protein corona of gold nanoparticles
Binding sites of albumins on gold nanoparticles were characterized by surface-enhanced Raman scattering.
Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart
Gaining knowledge on the biochemical profile of primary endothelial cells on a subcellular level can contribute to better understanding of cardiovascular disease.
ATR-FTIR spectroscopy shows changes in ovarian cancer cells after incubation with novel organoamidoplatinum(II) complexes
ATR-FTIR spectroscopy has been applied to compare the effect of new organoamidoplatinum(II) complexes with cisplatin on cells from a cisplatin-sensitive and a cisplatin-resistant ovarian cancer cell line.
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
Translating Raman spectroscopy for colorectal cancer diagnosis with a focus on high-throughput design, inter-user variability and sample handling considerations.
Dual excitation wavelength system for combined fingerprint and high wavenumber Raman spectroscopy
A probe-based Raman spectroscopy system using a single laser module with two excitation wavelengths has been developed for measuring the fingerprint and high wavenumber regions with a single detector.
New insights of Raman spectroscopy for oral clinical applications
Oral injuries are currently diagnosed by histopathological analysis of biopsy, which is an invasive procedure and does not give immediate results.
Raman spectroscopy of stored red blood cell concentrate within sealed transfusion blood bags
Spectral information relevant to the quality of stored blood can be obtained in situ through sealed blood transfusion bags using a commercially available instrument.
Near-field infrared nanospectroscopy and super-resolution fluorescence microscopy enable complementary nanoscale analyses of lymphocyte nuclei
First near-field infrared spectroscopy and imaging of lymphocyte nucleus at 30 nm spatial resolution reveals spectrochemically distinct regions in nuclear organization.
Raman spectroscopic analysis of high molecular weight proteins in solution – considerations for sample analysis and data pre-processing
This study explores the potential of Raman spectroscopy, coupled with multivariate regression techniques and ion exchange chromatography, to quantitatively monitor diagnostically relevant changes in high molecular weight proteins in liquid plasma.
Raman spectroscopy as a novel tool for fast characterization of the chemical composition of perivascular adipose tissue
One of the new targets of untapped therapeutic potential is perivascular adipose tissue (pVAT). Based on Raman spectroscopy we demonstrated that the lipid unsaturation degree was clearly distinct in various types of adipose tissues, in particular thoracic and abdominal pVATs, and was influenced by the age of animals.
Diagnosis of pancreatic cancer via1H NMR metabolomics of human plasma
The investigation of blood plasma via1H NMR metabolomics revealed a panel of potential biomarkers of pancreatic cancer.
Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model
PCA score of FTIR spectra in fingerprint region for cultured chondrocytes at day 7, 14 and 21 in HA hydrogel showing that the 3D culture drove chondrocytes (CY) to chondron-like (CN) structure dynamically.
Surface enhanced resonance Raman spectroscopy (SERRS) for probing through plastic and tissue barriers using a handheld spectrometer
Through tissue imaging of a live breast cancer tumour model using handheld surface enhanced resonance Raman spectroscopy (SERRS).
Blood-based near-infrared spectroscopy for the rapid low-cost detection of Alzheimer's disease
Plasma samples deposited on low-E slides were analysed in transmission mode by using a FT-NIR spectrometer in order to detect Alzheimer's disease using computer-based methods.
Non-invasive analysis of stored red blood cells using diffuse resonance Raman spectroscopy
A method to acquire the Raman spectra of sub-surface components using diffusely focused radiation in a microscope sampling configuration is described.
In vitro antibacterial activity of oxide and non-oxide bioceramics for arthroplastic devices: I. In situ time-lapse Raman spectroscopy
Raman spectroscopy proved why the antibacterial response of non-oxide Si3N4 bioceramic is superior to those of alumina-based oxide bioceramics.
Noninvasive and prospective diagnosis of coronary heart disease with urine using surface-enhanced Raman spectroscopy
The SERS spectra of human urine could provide valuable information for the noninvasive and prospective diagnosis of patients with suspected CHD.
An early investigative serum Raman spectroscopy study of meningioma
Serum Raman spectroscopy is explored to classify meningiomas, which are the most frequently reported non-malignant brain and CNS tumors and are often misdiagnosed. We find the technique can be prospectively extended to the screening of high-risk populations and for therapeutic monitoring.
FTIR spectroscopy of chronic venous leg ulcer exudates: an approach to spectral healing marker identification
Chronic venous leg ulcer arises as a venous insufficiency complication and is a cause of great morbidity.
Deciphering the biochemical similarities and differences among mouse embryonic stem cells, somatic and cancer cells using ATR-FTIR spectroscopy
Cellular macromolecules play important roles in cellular behaviors and biological processes.
Selecting optimal features from Fourier transform infrared spectroscopy for discrete-frequency imaging
Augmenting tissue histology with spectral information from mid-IR spectroscopy will benefit a lot. Optimal bands selection needed to do histopathological classification of mid-IR images will help to reduce imaging time and computation complexity. GPU use for feature selection makes it practical.
Detection of antibiotic resistant Escherichia Coli bacteria using infrared microscopy and advanced multivariate analysis
Determining E. coli bacteria susceptibility by analyzing their FTIR spectra using multivariate analysis.
Ultrafast laser-probing spectroscopy for studying molecular structure of protein aggregates
We report the development of a new technique to screen protein aggregation based on laser-probing spectroscopy with sub-picosecond resolution.
High definition infrared chemical imaging of colorectal tissue using a Spero QCL microscope
Mid-infrared microscopy has become a key technique in the field of biomedical science and spectroscopy. In this current study, we explore the use of a QCL infrared microscope to produce high definition, high throughput chemical images useful for the screening of biopsied colorectal tissue.
Development of a high throughput (HT) Raman spectroscopy method for rapid screening of liquid blood plasma from prostate cancer patients
High throughput Raman spectroscopy method for rapid and accurate diagnosis of prostate cancer using liquid plasma samples.
Near infrared spectroscopic assessment of developing engineered tissues: correlations with compositional and mechanical properties
Non-destructive near infrared spectroscopic data can be utilized for assessment of compositional and mechanical properties of engineered cartilage.
Ultra-filtration of human serum for improved quantitative analysis of low molecular weight biomarkers using ATR-IR spectroscopy
Monitoring of changes in the concentrations of the low molecular weight constituents enhanced by abundant proteins depletion.
SIproc: an open-source biomedical data processing platform for large hyperspectral images
There has recently been significant interest within the vibrational spectroscopy community to apply quantitative spectroscopic imaging techniques to histology and clinical diagnosis.
Early diagnosis of Alzheimer's disease using infrared spectroscopy of isolated blood samples followed by multivariate analyses
A simple blood test for the diagnosis of Alzheimer's disease using FTIR microscopy.
Virtual staining of colon cancer tissue by label-free Raman micro-spectroscopy
The great capability of virtual staining for label-free classification of colon cancer tissue has been demonstrated via Raman spectral imaging.
Raman spectroscopy and multivariate analysis for the non invasive diagnosis of clinically inconclusive vulval lichen sclerosus
The diagnostic performance of Raman spectroscopy for differentiating lichen sclerosus from other vulval conditions in fresh vulval biopsies is demonstrated.
Raman spectroscopy in microsurgery: impact of operating microscope illumination sources on data quality and tissue classification
A filter system to perform in vivo Raman spectroscopy measurements under microscope lighting for seamless integration into the surgical workflow.
Feature driven classification of Raman spectra for real-time spectral brain tumour diagnosis using sound
Spectroscopic diagnostics have been shown to be an effective tool for the analysis and discrimination of disease states from human tissue.
Computational cell analysis for label-free detection of cell properties in a microfluidic laminar flow
This method utilizes machine learning algorithms and fluid mechanic properties of cells in a microfluidic channel to find the compositions of cell mixtures.
Combining random forest and 2D correlation analysis to identify serum spectral signatures for neuro-oncology
Fourier transform infrared (FTIR) spectroscopy has long been established as an analytical technique for the measurement of vibrational modes of molecular systems.
Immunoassay for tumor markers in human serum based on Si nanoparticles and SiC@Ag SERS-active substrate
An immunoassay protocol is described to detect tumor markers in human serum based on a sandwich structure consisting of nano-Si immune probes and SiC@Ag SERS-active immune substrate.
Study of the biochemical effects induced by X-ray irradiations in combination with gadolinium nanoparticles in F98 glioma cells: first FTIR studies at the Emira laboratory of the SESAME synchrotron
One strategy to improve the clinical outcome of radiotherapy is to use nanoparticles as radiosensitizers.
Surface enhanced Raman spectroscopic direct determination of low molecular weight biothiols in umbilical cord whole blood
Biothiols are determined in whole blood samples by Surface Enhanced Raman Spectroscopy (SERS).
Non-invasive spectroscopy of transfusable red blood cells stored inside sealed plastic blood-bags
Spatially Offset Raman spectroscopy (SORS) has been used to retrieve biochemical information from red blood cell concentrate within sealed PVC bags, demonstrating the potential of SORS to non-invasively assess the condition of the cells prior to transfusion.
Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate
Sensing of methotrexate at clinically-relevant concentrations was achieved with a plasmon-coupling assay.
Direct-growth carbon nanotubes on 3D structural microelectrodes for electrophysiological recording
Direct growth of CNTs on 3D microelectrodes could detect distinguished zebrafish ECG resulting from the interfacial improvement analyzed by EIS.
Correction: Clinical applications of infrared and Raman spectroscopy: state of play and future challenges
About this collection
This collection of papers highlights fundamental research and applications of clinical spectroscopy published in analyst since 2017. The collection contains selected research and review articles in this field.