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Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart

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Abstract

Gaining knowledge on the biochemical profile of primary endothelial cells on a subcellular level can contribute to better understanding of cardiovascular disease. In this work, primary cardiac microvascular endothelial cells (CMECs) isolated from the mouse heart and murine H5V endothelial cell line were characterized with the use of a Raman imaging technique. Primary CMECs displayed a distinct Raman-based biochemical phenotype as compared with other cells isolated from the heart and were characterized by a low lipid content. In contrast to the murine H5V endothelial cell line, CMECs did not display lipid droplets (LDs) in the cytoplasm, while the former have many low-unsaturated LDs. In conclusion, Raman imaging is a fast and efficient tool to analyse single coronary endothelial cells in a non-invasive manner that can prove useful to characterize biochemical changes in a single isolated primary endothelial cell from a diseased heart.

Graphical abstract: Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart

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Publication details

The article was received on 15 Jul 2018, accepted on 01 Nov 2018 and first published on 05 Nov 2018


Article type: Paper
DOI: 10.1039/C8AN01308J
Citation: Analyst, 2019, Advance Article
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    Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart

    S. Tott, M. Grosicki, B. Klimas, D. Augustynska, S. Chlopicki and M. Baranska, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN01308J

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