Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges
Currently, there exists a multitude of different exosome isolation and characterization approaches based on biophysical and/or biochemical characteristics. Herein we review various technologies for separation of distinct exosome subpopulations.
Instrumentational implementation for parallelized nanopore electrochemical measurements
Nanopore electrochemistry, as one of the promising tools for single molecule sensing, has proved its capability in DNA sequencing and protein analysis.
Single enzyme electroanalysis
Traditional enzymology relies on the kinetics of millions of enzymes, an experimental approach that may wash out heterogeneities between individual enzymes. Electrochemical methods have emerged in the last 5 years to probe single enzyme reactivity.
Molecularly imprinted polymers for the detection of viruses: challenges and opportunities
Recent advancements regarding the application of MIPs and relevant virus imprinting techniques for the detection of viruses are highlighted.
Self-assembled nanomaterials for biosensing and therapeutics: recent advances and challenges
Self-assembled nanomaterials (SANs) exhibit designable biofunctions owing to their tunable nanostructures and modifiable surfaces.
Recent advances in bioanalytical methods to measure proteome stability in cells
This review summarizes recent bioanalytical methods for measuring and profiling protein stability in cells on a proteome-wide scale, which can provide insights for proteostasis and associated diseases.
DNA origami: an outstanding platform for functions in nanophotonics and cancer therapy
The utilization of the DNA origami technique in the fabrication of complex objects has given rise to possibilities in DNA-induced precise manufacturing and might open a new era of bottom-up fabrication.
Gas-propelled biosensors for quantitative analysis
Gas-propelled biosensors display a simple gas-based signal amplification with quantitative detection features based on the target recognition event in combination with gas propulsion.
Recent advances in the use of stimulated Raman scattering in histopathology
Stimulated Raman histopathology with computer aided diagnosis.
Progress in electrochemiluminescence of nanoclusters: how to improve the quantum yield of nanoclusters
Classification of nanoclusters and methods to improve their quantum yield and applications.
Harnessing bubble behaviors for developing new analytical strategies
Gas bubbles are easily accessible and offer many unique characteristic properties of a gas/liquid two-phase system for developing new analytical methods.
Biosensing based on upconversion nanoparticles for food quality and safety applications
The unique optical properties of UCNPs, in particular the ability for background-free optical detection, bestow great potential for food safety and quality monitoring.
H2O2/HOCl-based fluorescent probes for dynamically monitoring pathophysiological processes
H2O2/HOCl-based fluorescent probes for dynamically monitoring pathophysiological processes in vivo were described.
Aggregation-induced responses (AIR) of 2D-derived layered nanostructures enable emerging colorimetric and fluorescence sensors
Modulated aggregation and photonic properties of layered nanostructures (LNs) result in the stimuli-responsive output of colorimetric and fluorescence signals, contributing to the desired construction of novel chemical and biological sensors.
Phosphoproteomic strategies in cancer research: a minireview
Due to the critical nature of biological and clinical samples used in cancer research, in terms of amount, stability and diversity of analytes, several combinations of sample preparation steps and methods have been developed.
Microfluidic technologies for vasculature biomimicry
An overview of microfluidic technologies for vascular studies and fabrication of vascular structures.
Recent advances in reaction-based fluorescent probes for detecting monoamine oxidases in living systems
This Minireview summarizes the recent advances in reaction based MAO type fluorescent probes and their imaging applications in living systems.
Single cell transcriptomics: moving towards multi-omics
Single-cell multi-omics analysis helps characterize multiple layers of molecular features at a single-cell scale to provide insights into cellular processes and functions.
Recent advances in and potential utilities of paper-based electrochemical sensors: beyond qualitative analysis
Paper based electrochemical sensors (PESs) are simple, low-cost, portable and disposable analytical sensing platforms that can be applied in clinical diagnostics, food quality control and environmental monitoring.
A new point-of-care test for the diagnosis of infectious diseases based on multiplex lateral flow immunoassays
Multiplex lateral flow immunoassays for outstanding POCT of infectious diseases.
Advances in diagnostic methods for keloids and biomarker-targeted fluorescent probes
A focused review of keloid diagnosis, keloid biomarkers, and recently reported fluorescent probes.
Emerging trends in sensors based on carbon nitride materials
A new class of functional materials, carbon nitrides, has recently attracted the attention of researchers.
New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells
We provide a vision for integrating pioneering mass spectrometry technologies for future applications in single cell analysis.
Advances in mass spectrometry based single-cell metabolomics
Single cell metabolomics using mass spectrometry can contribute to understanding biological activities in health and disease.
Quantitative imaging of lipid droplets in single cells
Non-destructive spatial characterization of lipid droplets using coherent Raman scattering microscopy and computational image analysis algorithms at the single-cell level.
Analysing single live cells by scanning electrochemical microscopy
Scanning electrochemical microscopy (SECM) offers single live cell activities along its topography toward cellular physiology and pathology.
Recent advances in single cell manipulation and biochemical analysis on microfluidics
This review highlights the advances in single cell manipulation and biochemical analysis on microfluidics over the past three years.
Advancements in fractal plasmonics: structures, optical properties, and applications
Fractal nanostructures exhibit optical properties that span the visible to far-infrared and are emerging as exciting structures for plasmon-mediated applications.
Prospective on using fibre mid-infrared supercontinuum laser sources for in vivo spectral discrimination of disease
Mid-infrared (MIR) fibre-optics may play a future role in in vivo diagnosis of disease, including cancer.
Synthesis, design and sensing applications of nanostructured ceria-based materials
Cerium-based materials possess redox properties due to the presence of dual valence states of Ce3+ and Ce4+.
Investigations on the interface of nucleic acid aptamers and binding targets
The binding targets of aptamers, the interaction probing methods, and the main binding forces mediating the interaction have been summarized.
Lipid metabolism in inflammation-related diseases
Lipidomics is used to describe the complete lipid profile and network of cellular lipid metabolism. Traditionally, lipids are recognized as general membrane construction and energy storage molecules. Now, lipids are regarded as potent signaling molecules that regulate a multitude of cellular responses.
Luminescent switch sensors for the detection of biomolecules based on metal–organic frameworks
The applications of MOFs-based luminescent sensors for biomolecules sensing via a “turn on” or “turn off” response have been reviewed.
Direct label-free imaging of brain tissue using synchrotron light: a review of new spectroscopic tools for the modern neuroscientist
A suite of direct spectroscopic imaging techniques using synchrotron light can provide increased biochemical insight into brain disease.
Chemical clocks, oscillations, and other temporal effects in analytical chemistry: oddity or viable approach?
We review chemo-chronometric assays, in which the time factor—rather than analogue signals—is used to determine concentrations of analytes.
Platforms for rapid cancer characterization by ambient mass spectrometry: advancements, challenges and opportunities for improvement towards intrasurgical use
Ambient Mass Spectrometry (MS) analysis is widely used to characterize biological and non-biological samples.
Recent advances in merging photonic crystals and plasmonics for bioanalytical applications
The integration of photonic crystals and plasmonics enhances the performance of bioanalytical applications.
How iMALDI can improve clinical diagnostics
Protein mass spectrometry (MS) is an indispensable tool to detect molecular signatures that can be associated with cellular dysregulation and disease.
Biosensing strategies based on enzymatic reactions and nanoparticles
Application of new nanomaterials to detection of enzymatic activities allows the development of new sensitive and selective bioanalytical assays based on enzymes for recognition and signal amplification.
Mobile diagnostics: next-generation technologies for in vitro diagnostics
The emergence of a wide range of applications of smartphones along with advances in ‘liquid biopsy’ has significantly propelled medical research particularly in the field of in vitro diagnostics (IVD).
Gas-generating reactions for point-of-care testing
Gas generation-based measurement is an attractive alternative approach for POC (Point-of-care) testing, which relies on the amount of generated gas to detect the corresponding target concentrations.
Chem/bio sensing with non-classical light and integrated photonics
Modern quantum technology experiences currently extensive advances in its applicability in communications, cryptography, computing, metrology and lithography. Harnessing this technology platform for chem/bio sensing scenarios is an appealing possibility to enable ultra-sensitive detection schemes.
Sample preparation for polar metabolites in bioanalysis
Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics where maximum information has to be obtained without spoiling the analytical instrument. The sample extraction of polar metabolites is still challenging but strategies exist to enable the phase transfer of hydrophilic metabolites from the biological phase to a clean interference-free phase.
Synthesis and modification of carbon dots for advanced biosensing application
Nucleic acid amplification-based microfluidic approaches for antimicrobial susceptibility testing
This review discusses the recent developments in microfluidics technology for antimicrobial susceptibility testing with a special emphasis on the nucleic acid amplification-based approach.
Chemometrics in forensic science: approaches and applications
This tutorial review provides an overview of common chemometric methods, and their potential uses within forensic science for evidence interpretation and optimisation of analytical procedures.
Approaches in metabolomics for regulatory toxicology applications
This tutorial review provides an overview of key concepts in metabolomics and their application to develop successful strategies in regulatory toxicology-oriented studies.
Raman microscopy for skin evaluation
The applications of Raman microscopies, such as spontaneous Raman scattering microscopy and coherent Raman scattering microscopy, used to evaluate skin and research trends are presented.
Applications of DNA-nanozyme-based sensors
In this review, the research progress of the sensors based on the DNA-nanozymes were summarized.
True one cell chemical analysis: a review
The constantly growing field of True One Cell (TOC) analysis has provided important information on the direct chemical composition of various cells and cellular components.
Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations
Quantifying and regulating oxygen in a microphysiological models can be achieved via an array of technologies, and is an essential component of recapitulating tissue-specific microenvironments.
Fluorescent organic nanoparticles (FONs) as convenient probes for metal ion detection in aqueous medium
Fluorescent organic nanoparticle (FON)-based chemosensors are emerging as a valuable tool for the fast and accurate detection of metal ions in aqueous media. In this review, we highlight the recent developments in this field.
A unified approach for investigating chemosensor properties – dynamic characteristics
The response is what makes a sensor. In this review the methods for characterizing the response are laid out.
A pressure-driven gas-diffusion/permeation micropump for self-activated sample transport in an extreme micro-environment
The pressure-driven gas-diffusion/permeation micropump is highlighted for stable microdroplet/liquid delivery under extreme conditions, e.g. high temperature, and a three-dimensional, long-distance and complex-topology microchannel.
Single-system based discriminative optical sensors: different strategies and versatile applications
Single-system based optical sensors can realize discrimination through multi-wavelength cross-reactive responses by different strategies.
Advances in the integration of quantum dots with various nanomaterials for biomedical and environmental applications
We review the advances in the integration of quantum dots with various nanomaterials for biomedical and environmental applications.
Advances in point-of-care diagnostic devices in cancers
The early diagnosis and monitoring of the progress of cancers are limited due to the lack of adequate screening tools.
Chemical analysis in saliva and the search for salivary biomarkers – a tutorial review
A review of the uses of saliva biomarkers, detection methods and requirements for new biomarkers.
Commercially available rapid diagnostic tests for the detection of high priority pathogens: status and challenges
Rapid diagnostic tests (RDTs) will provide a key element of disease surveillance. Their rapid turnaround, low cost, and accessibility in resource limited areas will help increase public health reporting and facilitate outbreak containment.
Spatial proteomics for understanding the tissue microenvironment
We outline the achievements and remaining challenges of mass spectrometry-based tissue spatial proteomics. Exciting technology developments along with important biomedical applications of spatial proteomics are highlighted.
Selective-sampling Raman imaging techniques for ex vivo assessment of surgical margins in cancer surgery
Selective-sampling Raman Imaging enables intraoperative assessment of excised surgical margins in cancer surgery, a review.
Nucleic acid analysis on paper substrates (NAAPs): an innovative tool for Point of Care (POC) infectious disease diagnosis
The cost-effective rapid diagnosis of infectious diseases is an essential and important factor for curing such diseases in the global public health care picture.
Smartphone-based optical analysis systems
The review describes the design, application and performance of current smartphone-based colorimeters, photo- and spectrometers and fluorimeters. Furthermore, it gives an overview of the advantages and disadvantages of such systems.
Electroanalytical overview: the pungency of chile and chilli products determined via the sensing of capsaicinoids
We explore the endeavours directed to the development of electrochemical-based sensors for the determination of capsaicin and related compounds, starting from their use in hyphenated laboratory set-ups to their modern use as stand-alone electroanalytical sensors.
Carbon dots as naked eye sensors
Optical sensors are always fascinating for chemists due to their selectivity, sensitivity, robustness and cost-effective nature.
Microfluidics for sepsis early diagnosis and prognosis: a review of recent methods
Sepsis is a complex disorder of immune system response to infections that can be caused by a wide range of clinical contexts.
Advances in gold nanoparticles for mycotoxin analysis
AuNPs have been widely used in mycotoxin detection including ELISA, optical-based sensors, electrochemical-based sensors, surface-enhanced Raman scattering-based sensors, mass-based sensors and other sensors.
Rapid developments in lateral flow immunoassay for nucleic acid detection
Schematic of two main research directions of lateral flow assays for nucleic acid detection.
Wearable sensors for continuous oral cavity and dietary monitoring toward personalized healthcare and digital medicine
Recent advances in wearable sensors for dietary monitoring and saliva analysis are presented to discuss super-aged/aging societies, non-face-to-face social life, and global pandemic disease issues toward personalized healthcare and digital medicine.
Chemical sensors for selective and quantitative heparin sensing
In this review article, chemical sensors for selective and quantitative heparin sensing are discussed with detailed examples.
About this collection
This themed collection features recent review articles published in Analyst.