Fluorescent probes for organelle-targeted bioactive species imaging
The dynamic fluctuations of bioactive species in living cells are associated with numerous physiological and pathological phenomena. The emergence of organelle-targeted fluorescent probes has significantly facilitated our understanding on the biological functions of these species. This review describes the design, applications, challenges and potential directions of organelle-targeted bioactive species probes.
In-depth structural analysis of glycans in the gas phase
Although there have been substantial improvements in glycan analysis over the past decade, the lack of both high-resolution and high-throughput methods hampers progress in glycomics.
Electrochemical imaging of cells and tissues
This minireview summarizes the recent achievements of electrochemical imaging platforms to map cellular functions in biological specimens using electrochemical scanning nano/micro-probe microscopy and 2D chips containing microelectrode arrays.
Recent advances in near-infrared II fluorophores for multifunctional biomedical imaging
A review of recent advances in near-infrared II fluorophores for multifunctional biomedical imaging.
Endoplasmic reticulum targeting fluorescent probes to image mobile Zn2+
Two endoplasmic reticulum (ER) targeting probes were developed to image mobile Zn2+ to help understand Zn2+ related biological processes in the ER.
A “Double-Locked” and enzyme-activated molecular probe for accurate bioimaging and hepatopathy differentiation
A “double-locked” and enzyme-activated molecular fluorescent probe for accurate bioimaging and hepatopathy differentiation.
Rational design of a multifunctional molecular dye for dual-modal NIR-II/photoacoustic imaging and photothermal therapy
Theranostic nanoprobes (SYL NPs) prepared from a newly designed NIR-II fluorophore and FDA approved DSPE-mPEG5000 were well tolerated and exhibited multifunctional potential in diagnosis (photoacoustic & fluorescence imaging) and phototherapy in animal models.
Mitochondria-targeting fluorescent molecules for high efficiency cancer growth inhibition and imaging
5BMF is a new fluorescent mitochondria-accumulating delocalized lipophilic cations [DLC] that boasts significantly increased anti-cancer effects and low toxicity in comparison to previous DLCs, addressing current hurdles in DLC clinical translation.
A molecular design strategy toward enzyme-activated probes with near-infrared I and II fluorescence for targeted cancer imaging
A molecular design strategy is established to access diverse enzyme-activated probes that are excitable and emit in the NIR (I and II) region with favorable Stokes shifts, enabling targeted cancer imaging by real-time monitoring enzyme activities.
Diketopyrrolopyrrole-based fluorescence probes for the imaging of lysosomal Zn2+ and identification of prostate cancer in human tissue
Diketopyrrolopyrrole-based fluorescent probes have been developed for the detection of low pH and Zn2+ in an AND logic fashion. They can discriminate between cancerous and healthy prostate tissue.
Evaluation of HOCl-generating anticancer agents by an ultrasensitive dual-mode fluorescent probe
Probe C7 for the first time presents different modes of fluorescence signals to both nanomolar and micromolar concentrations of HOCl.
Telomerase and poly(ADP-ribose) polymerase-1 activity sensing based on the high fluorescence selectivity and sensitivity of TOTO-1 towards G bases in single-stranded DNA and poly(ADP-ribose)
Telomerase and poly(ADP-ribose) polymerase-1 (PARP-1) are two potential cancer biomarkers and are closely related to tumor initiation and malignant progression.
Direct sequencing of 2′-deoxy-2′-fluoroarabinonucleic acid (FANA) using nanopore-induced phase-shift sequencing (NIPSS)
The first demonstration of direct sequencing of 2′-deoxy-2′-fluoroarabinonucleic acid (FANA) using Nanopore-Induced Phase-Shift Sequencing (NIPSS).
Collision cross section compendium to annotate and predict multi-omic compound identities
The Unified Compendium is an online interactive tool that utilizes ion mobility collision cross sections to annotate biochemical molecules.
An enzyme-activatable probe liberating AIEgens: on-site sensing and long-term tracking of β-galactosidase in ovarian cancer cells
We describe an enzyme-regulated liberation strategy to in situ generate AIEgen nanoaggregates for on-site sensing and long-term tracking of β-galactosidase in ovarian cancer cells.
Rational design of a super-contrast NIR-II fluorophore affords high-performance NIR-II molecular imaging guided microsurgery
A super-contrast NIR-II fluorophore IR-BEMC6P with enhanced quantum yield is developed and the excretion mechanism is identified.
Molecular design of near-infrared fluorescent Pdots for tumor targeting: aggregation-induced emission versus anti-aggregation-caused quenching
This article presents the first work to fundamentally study the effect of steric hindrance in Pdot systems.
A long-lived peptide-conjugated iridium(III) complex as a luminescent probe and inhibitor of the cell migration mediator, formyl peptide receptor 2
Formyl peptide receptors play important biological and therapeutic roles in wound repair and inflammatory diseases.
Combined reversible switching of ECD and quenching of CPL with chiral fluorescent macrocycles
A series of chiral fluorescent macrocycles display a remarkable combination of both +/– ECD and strong on/off CPL reversible switching upon cation binding and displacement.
Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis
Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of keloid diseases.
Extraordinary water adsorption characteristics of graphene oxide
The laminated structure of graphene oxide (GO) confers unique interactions with water molecules which may be utilised in a range of applications that require materials with tuneable hygroscopic properties.
Translating molecular detections into a simple temperature test using a target-responsive smart thermometer
A novel target-responsive smart thermometer for translating molecular detection into a temperature test has been developed.
ROS scavenging Mn3O4 nanozymes for in vivo anti-inflammation
Reactive oxygen species (ROS) scavenging Mn3O4 nanozymes effectively protected live mice from ROS-induced ear-inflammation in vivo.
Fabrication of microstructured binary polymer brush “corrals” with integral pH sensing for studies of proton transport in model membrane systems
Binary polymer brush microstructures incorporating ratiometric fluorescent pH indicators enable in situ studies of light-activated transmembrane proton transport by proteorhodopsin.
Novel dual-function near-infrared II fluorescence and PET probe for tumor delineation and image-guided surgery
The first small-molecule based αvβ3-targeted NIR-II/PET dual-modal probes via base-catalyzed thiol-addition chemistry were concisely assembled and evaluated.
High throughput reaction screening using desorption electrospray ionization mass spectrometry
We report the high throughput analysis of reaction mixture arrays using methods and data handling routines that were originally developed for biological tissue imaging.
Cell imaging of dopamine receptor using agonist labeling iridium(III) complex
A long-lived complex 13 could selectively bind to dopamine receptors (D1R/D2R) and monitor their internalization in living cells.
Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level
We demonstrate the simultaneous detection of human 8-oxoguanine DNA glycosylase 1 and human alkyladenine DNA glycosylase at the single-molecule level.
Deformylation reaction-based probe for in vivo imaging of HOCl
We report a near-infrared emissive probe which can detect HOCl in vivo by both fluorescence imaging and the naked eye.
Construction of an autonomously concatenated hybridization chain reaction for signal amplification and intracellular imaging
The concatenated hybridization chain reaction (C-HCR) was constructed as a versatile and robust tool for signal amplification and intracellular imaging, which was attributed to the synergistic amplification effect between HCR-1 and HCR-2.
About this collection
This specially curated collection pulls together some of the most popular articles from 2018 and 2019 in the field of analytical chemistry. The collection presents some outstanding contributions to the field, ranging from high throughput reaction screening using desorption electrospray ionization mass spectrometry to a review of fluorescent probes for organelle-targeted bioactive species imaging, and as with all Chemical Science articles - they are all completely free to access and read. We hope you enjoy browsing through this collection.