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Endoplasmic reticulum targeting fluorescent probes to image mobile Zn2+

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Abstract

Zn2+ plays an important role in the normal function of the endoplasmic reticulum (ER) and its deficiency can cause ER stress, which is related to a wide range of diseases. In order to provide tools to better understand the role of mobile Zn2+ in ER processes, the first custom designed ER-localised fluorescent Zn2+ probes have been developed through the introduction of a cyclohexyl sulfonylurea as an ER-targeting unit with different Zn2+ receptors. Experiments in vitro and in cellulo show that both probes have a good fluorescence switch on response to Zn2+, high selectivity over other cations, low toxicity, ER-specific targeting ability and are efficacious imaging agents for mobile Zn2+ in four different cell lines. Probe 9 has been used to detect mobile Zn2+ changes under ER stress induced by both tunicamycin or thapsigargin, which indicates that the new probes should allow a better understanding of the mechanisms cells use to respond to dysfunction of zinc homeostasis in the ER and its role in the initiation and progression of diseases to be developed.

Graphical abstract: Endoplasmic reticulum targeting fluorescent probes to image mobile Zn2+

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Publication details

The article was received on 26 Aug 2019, accepted on 16 Oct 2019 and first published on 16 Oct 2019


Article type: Edge Article
DOI: 10.1039/C9SC04300D
Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Endoplasmic reticulum targeting fluorescent probes to image mobile Zn2+

    L. Fang, G. Trigiante, R. Crespo-Otero, C. S. Hawes, M. P. Philpott, C. R. Jones and M. Watkinson, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C9SC04300D

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