Themed collection Most popular 2021 analytical chemistry articles
DNA nanostructure-based nucleic acid probes: construction and biological applications
In recent years, DNA has been widely noted as a kind of material that can be used to construct building blocks for biosensing, in vivo imaging, drug development, and disease therapy because of its advantages of good biocompatibility and programmable properties.
Fluorescent small organic probes for biosensing
Small-molecule based fluorescent probes are increasingly important for the detection and imaging of biological signaling molecules due to their simplicity, high selectivity and sensitivity, whilst being non-invasive, and suitable for real-time analysis of living systems.
Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications
This review highlights the molecular design strategy of β-galactosidase-activatable probes from turn-on mode to ratiometric mode, from ACQ to AIE-active probes, from NIR-I to NIR-II imaging and dual-mode of chemo-fluoro-luminescence imaging.
CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) protein systems revolutionize genome engineering and advance analytical chemistry and diagnostic technology.
Phosphorescent metal complexes as theranostic anticancer agents: combining imaging and therapy in a single molecule
The recent development in phosphorescent iridium, ruthenium and rhenium complexes as theranostic anticancer agents is summarized.
Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells
Acridonylalanine (Acd) is photostable, with a high quantum yield and long fluorescence lifetime in water. An evolved tRNA synthetase (RS) enables genetic incorporation of Acd in mammalian cells and its use in fluorescence lifetime imaging microscopy.
A highly sensitive and selective fluoride sensor based on a riboswitch-regulated transcription coupled with CRISPR-Cas13a tandem reaction
A fluoride sensor based on riboswitch-regulated transcription coupled with Cas13a sensor can detect fluoride in water with a portable fluorometer. This sensor expands nuclei acid sensors to an anion, with high sensitivity and selectivity against other common anions.
Turning waste into wealth: facile and green synthesis of carbon nanodots from pollutants and applications to bioimaging
The pollutant reactive red 2 was employed to synthesize fluorescent carbon nanodots allowing biological imaging in vitro and in vivo.
Natural products dereplication by diffusion ordered NMR spectroscopy (DOSY)
We report accurate DOSY NMR based molecular weight and diffusion coefficient prediction tools. These tools can be used to dereplicate known natural products from databases using structurally rich NMR data as a surrogate for mass spectrometric data.
A plasmon-enhanced theranostic nanoplatform for synergistic chemo-phototherapy of hypoxic tumors in the NIR-II window
A plasmon-enhanced theranostic nanoplatform for synergistic chemo-phototherapy (PTT/PDT) of hypoxic tumors in the NIR-II window.
Activation of apoptosis by rationally constructing NIR amphiphilic AIEgens: surmounting the shackle of mitochondrial membrane potential for amplified tumor ablation
In this contribution, based on a “step-by-step” molecular design strategy, a novel NIR amphiphilic AIEgen TPA-S-TPP with a triplet lifetime of 11.43 μs and surmounting the shackle of MMP was successfully fabricated for amplified tumor ablation.
NIR-II cell endocytosis-activated fluorescent probes for in vivo high-contrast bioimaging diagnostics
A Cell Endocytosis-Activated Fluorescent (CEAF) probe triggered by disaggregation and protonation is designed for high contrast in vivo bioimaging and diagnostics in the second near-infrared window (1000–1700 nm).
A fluorescent probe for the discrimination of oxidation states of palladium
A simple porphyrin–coumarin conjugate PPIX-L2 was developed for the discrimination of different oxidation states of palladium (Pd0 and Pd2+), and with a significantly improved sensitivity.
Expanded porphyrins: functional photoacoustic imaging agents that operate in the NIR-II region
In this study, two expanded porphyrins, octaphyrin and naphthorosarin were evaluated as potential PA agents. The nanoparticle encapsulation of octaphyrin successfully enabled the visualization of acidic environments and the discrimination between cancerous and healthy tissues.
A therapeutic keypad lock decoded in drug resistant cancer cells
Information processing therapeutics with an implemented keypad lock logic gate selects input order for activation in drug resistant cancer cells.
Strategic engineering of alkyl spacer length for a pH-tolerant lysosome marker and dual organelle localization
A series naphthalimide-based fluorophores were designed by alkyl spacer length engineering to discover a pH-tolerant lysosomal marker. This approach also allows to probe lysosome-related organelles in C. elegans and communication between organelles.
A dual-caged resorufin probe for rapid screening of infections resistant to lactam antibiotics
This work reports a dual-caged fluorogenic probe and its demonstration for rapid and sensitive screening of as low as 1000 clinically isolated β-lactamase-expressing bacteria per milliliter urine samples within 2 hours.
Translating daily COVID-19 screening into a simple glucose test: a proof of concept study
COVID-19 glucose test: translating SARS-CoV-2 detection into a glucose test is achieved by incorporating target-responsive rolling circle amplification and a CRISPR-based collateral cleavage module with a portable glucose meter.
Rapid organocatalytic chirality analysis of amines, amino acids, alcohols, amino alcohols and diols with achiral iso(thio)cyanate probes
Organocatalysis with a simple arylisocyanate probe enables accelerated optical concentration and enantiomeric ratio determination of a large variety of chiral compounds based on straightforward UV/CD analysis.
Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
Blue-conversion, a photooxidative conversion leading to the hypsochromic shift of absorption and emission spectra, occurs in popular organic dyes under conventional laser illumination and produces severe artifacts in multicolor fluorescence imaging.
X-ray scattering reveals ion clustering of dilute chromium species in molten chloride medium
Ion clustering of dilute chromium species was unexpectedly revealed in a high-temperature molten chloride salt, challenging several long-held assumptions regarding specific ionic interactions and transport in molten ionic media.
A facile combinatorial approach to construct a ratiometric fluorescent sensor: application for the real-time sensing of cellular pH changes
A combinatorial assembly of two types of intensity-based fluorophores on a DNA nanostructure provided a ratiometric pH probe with high emission intensity for monitoring intracellular pH changes.
Late-stage functionalisation of alkyne-modified phospha-xanthene dyes: lysosomal imaging using an off–on–off type of pH probe
A series of NIR-emissive phospha-xanthene dyes bearing an ethynyl group are reported. The late-stage functionalisation of the NIR dyes enables creation of multi-functionalised fluorescent probes that can be designed to target organelles of interest.
Synthesis of an AIEgen functionalized cucurbituril for subcellular bioimaging and synergistic photodynamic therapy and supramolecular chemotherapy
An AIEgen-functionalized cucurbituril was synthesized for the first time and spontaneously self-assembled into nanoaggregates in aqueous solutions and allowed subcellular imaging of the lysosome and photodynamic therapy and chemotherapy of cancer.
Clicking of organelle-enriched probes for fluorogenic imaging of autophagic and endocytic fluxes
A new strategy is developed for direct fluorogenic imaging of autophagic and endocytic fluxes via clicking of organelle-enriched trans-cyclooctenol and tetrazine derived probes.
Multiplexed optical barcoding of cells via photochemical programming of bioorthogonal host–guest recognition
Barcoding provides abilities to learn about individual species within an apparently homogeneous population. We describe a light-mediated multiplexed cellular barcoding strategy through spatial programming of cucurbituril molecular recognition.
Rapid and ultrasensitive electrochemical detection of circulating tumor DNA by hybridization on the network of gold-coated magnetic nanoparticles
This study introduces a new electrochemical sensing strategy for the rapid detection of circulating tumor DNA (ctDNA) from whole blood in combination with a network of DNA-Au@MNPs with high sensitivity and excellent selectivity.
Design and synthesis of a ratiometric photoacoustic imaging probe activated by selenol for visual monitoring of pathological progression of autoimmune hepatitis
A ratiometric photoacoustic imaging probe activated by selenol was developed for visual monitoring of pathological progression of autoimmune hepatitis.
Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations
We report on a study that combines advanced fluorescence methods with molecular dynamics simulations to cover timescales from nanoseconds to milliseconds for a large protein, the chaperone Hsp90.
A rapid and sensitive method for chiroptical sensing of α-amino acids via click-like labeling with o-phthalaldehyde and p-toluenethiol
A highly practical method for comprehensive chiroptical sensing of free α amino acids with streamlined operation and high sensitivity via dual CD/UV measurements is developed.
About this collection
This specially curated collection pulls together some of the most popular articles from 2021 in the field of analytical chemistry. The collection presents some outstanding contributions to the field, ranging from fluorescent small organic probes for biosensing to a therapeutic keypad lock decoded in drug resistant cancer cells, and as with all Chemical Science articles – they are all completely free to access and read. We hope you enjoy browsing through this collection.