Issue 10, 2021

Fluorescent small organic probes for biosensing


Small-molecule based fluorescent probes are increasingly important for the detection and imaging of biological signaling molecules due to their simplicity, high selectivity and sensitivity, whilst being non-invasive, and suitable for real-time analysis of living systems. With this perspective we highlight sensing mechanisms including Förster resonance energy transfer (FRET), intramolecular charge transfer (ICT), photoinduced electron transfer (PeT), excited state intramolecular proton transfer (ESIPT), aggregation induced emission (AIE) and multiple modality fluorescence approaches including dual/triple sensing mechanisms (DSM or TSM). Throughout the perspective we highlight the remaining challenges and suggest potential directions for development towards improved small-molecule fluorescent probes suitable for biosensing.

Graphical abstract: Fluorescent small organic probes for biosensing

Article information

Article type
19 Dec 2020
16 Jan 2021
First published
18 Jan 2021
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2021,12, 3406-3426

Fluorescent small organic probes for biosensing

X. Tian, L. C. Murfin, L. Wu, S. E. Lewis and T. D. James, Chem. Sci., 2021, 12, 3406 DOI: 10.1039/D0SC06928K

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