Themed collection Analytical Methods Recent HOT articles

A turn-on fluorescent probe for cysteine with high selectivity was designed and synthetized. The probe can be used for “naked-eye” detection of Cys with an obvious color change from yellow to colorless. The probe could be applied for Cys detection in real human serum.

A deep-red emission fluorescent probe HY for detecting viscosity in living systems was developed. The probe exhibited ideal properties including good selectivity, high stability and low cytotoxicity. Importantly, the probe HY has been successfully utilized for monitoring viscosity variations in cells and living mice.

Based on the force tracing technique, the dynamic trans-membrane transport process of single SiNPs in living cells was monitored.

Human papillomavirus (HPV), an important causative factor for cervical cancer, alters lipid metabolism.

Most of the current electrochemical sensors for the detection of nitroaromatic explosives directly exploit the reduction of nitro groups at the electrode.

Fluorescence assay of low levels of biothiols, such as cysteine and homocysteine, based on hydroxyapatite nanoparticles is reported.

A label-free colorimetric aptasensor that uses PNA/DNA hybrids and a cyanine dye (DiSC₂(5)) was developed for highly sensitive and selective detection of proteins in aqueous solution.

The mechanical properties of different pulp fibres in liquid were measured using an atomic force microscope.

Using commercially available personal glucose meters for the determination of hexokinase activity.

A FRET aptasensor based on a novel donor–acceptor pair between ZnS QDs and Ag nanocubes was developed for AFB1 detection.

Raman spectroscopy in conjunction with deep learning and non-negative least squares method was proposed as a solution to overcome the drug fast screening of lactose dominated drug formulations.

One-step electrochemically reduced graphene oxide with high surface area and improved electron transfer kinetics shows great performances in the determination of furfural in dairy milk.

A new adhesive was employed to fix HLB sorbent in stir-bars, providing high chemical/mechanical resistance and appropriate performance.

Self-built hybrid electronic nose prototypes equipped with organic–inorganic nanocomposite gas sensors and metal-oxide semiconductor gas sensors for bacterial discrimination.

A transparent and conducting FTO coated glass substrate was modified with AuNP-anchored conducting PCG. The AuNP/PCG/FTO sensor was applied for catalytic oxidation of NO₂⁻ with high selectivity, sensitivity, stability, and a low limit of detection.

As(III) pollution has caused increasing concern due to its significant impact in environmental safety and human health.

Au-4MBA@Ag and Au-XP013@Ag were successfully synthesized by a direct reduction method. After coupling the antibody, the simultaneous quantitative detection of cTnI and H-ABP was achieved by detecting the signal on the magnetic beads.

TD-DART-MS was shown to accurately and sensitively detect a wide range of benzodiazepines.

A simple and facile self-assemble strategy is developed to prepare C-dots/gold nanoclusters dual-emission ratiometric optical thermometry in living cells.

The probe DN exhibited excellent selectivity and sensitivity towards ClO⁻, and it was successfully used for fluorescence imaging of ClO⁻ in living cells.

A large volume sampling technique is used to develop and validate a home-made SPE system connected to a particle collector in situ pump for high sample pre-concentration and determination of petroleum hydrocarbons and their ¹³C/¹²C isotope ratio.

Monodisperse anisotropic (AuNR@Ag)–PANI JNPs were synthesized by using a droplet-based microfluidic platform, and show a fairly good response to Hg²⁺ ions and have been successfully applied for quantitative analysis of Hg²⁺ ions in real water samples.

An ultrasensitive label-free photoelectrochemical sensor based on Ag₂O-sensitized WO₃/TiO₂ acicular composite for AFB1 detection.

This work describes, for the first time, an in situ silanization process for creating phenyl–butyl gradients on particle LC columns.

Utilization of chicken anti-protein A IgY as an antibody pair for sensitive and selective detection of S. aureus.

Gas chromatographic (GC) determination of quantitative fatty acid composition: which derivatization reagent is the best?

A series of azonia cyanine dyes were designed and synthesized for nucleic acid detection and nucleolus imaging in fixed HeLa cells.

Internal standardization and MEC can be used successfully to correct for matrix effects in direct analysis of urine by ICP OES.

The flow of Mask R-CNN model for processing digital polymerase chain reaction (dPCR) fluorescence images.

The LIBS technique coupled with VIM-RF was applied for the rapid analysis of the acidity of iron ore.

A simple, low-cost and one-step hydrothermal treatment for the synthesis of molybdenum disulfide quantum dots (MoS₂ QDs) is proposed. They exhibit excellent PL properties and can be promising probes for sensing of hydroquinone and bioimaging.

An olfactory visualization sensor system was developed to verify the feasibility of the in situ monitoring of yeast fermentation stages with a pattern recognition algorithm.

H₂S is an important signaling molecule in living systems and participates in a series of physiological and pathological processes.

An efficient method has been found to discriminate different brands of packaged water which achieves to detect counterfeit products in the packaged water market.

The detection of hydrogen sulfide (H₂S) is important due to its role in the diagnosis of many diseases since H₂S is involved in the protection of neurons from oxidative stress and neuronal transmission modulation in the brain.

An I-shaped DNA assembly structure coupled with endonuclease-assisted isothermal amplification strategy was constructed for sensitive and fast detection of Hg(II).

A new ratiometric fluorescent probe for the detection of water in organic solvents with low limit of detection and large Stokes shift.

The goal of the current work was to develop a simple and sensitive high-performance liquid chromatography in combination with the fluorescence detection (HPLC-FLD) method for the fluorescence turn-on determination of cyanide and thiocyanate in swine plasma.

A fluorescent probe NaP which can image endogenous formaldehyde in living cells and quantitatively detect basal formaldehyde in milk samples.

A simple and enzyme-free electrochemical immunoassay was developed for the sensitive screening of cancer antigen 125 using anodic-stripping voltammetry.

Organic dye detection requires high sensitivity, low-cost equipment, simplified procedures, and real-time capabilities; thus, electrochemical methods are gradually emerging in this field instead of chromatography.

A 2D-molecularly imprinted colloidal array was fabricated for the visual detection of clindamycin hydrochloride and the LOD is 0.01 mM.

A simple, sensitive, and biological safety POCL–GOx–alginate/Ca gel system to detect and image local concentration of glucose in tumor periphery.

Novel nitrogen co-doped carbon dots (NCDs) were synthesized as a fluorescent “on–off–on” switch for the highly sensitive and selective sensing of Cu²⁺ and glutathione (GSH) by a straightforward pyrolysis route.

A novel fluorescence signal-on biosensing platform was developed by using photoinduced electron transfer (PET) between copper nanoclusters (CuNCs) and G-quadruplex/hemin.

One in every 20 people develops kidney stones at some point in their life.

Herein, a new near-infrared turn-on fluorescent probe, YPT, was designed and synthesized for the rapid detection of Zn²⁺, which exhibited low limit of detection for Zn²⁺ (12 nM) as well as a favorable large Stokes shift (λ_em − λ_ex = 168 nm).

A new yet little understood threat to our ecosystems is microplastics.

Two straightforward methods for glyphosate and AMPA analysis in honey through ion chromatography coupled to Q-Orbitrap accurate high-resolution mass spectrometry as well as their application to real samples are presented.

In this research, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and colloidal gold-based immunochromatographic (GICG) strip were developed for the detection of the antiviral drug amantadine (AM) in animal-derived foods.

The choice of blood specimens is still disputable in metabonomic studies due to the great metabolic diversities of serum and plasma.

A novel fluorescence sensing platform based on boron and nitrogen co-doped graphene quantum dots (B,N-GQDs) was developed and used to detect the concentration of both Hg²⁺ and F⁻ ions.

Cysteine (Cys) has emerged as a significant sensing target in recent years.

Portable, cost-effective and quantitative detection of various targets related to health at home and in the field has the potential to revolutionize medical diagnostics.

Detection of autologous blood doping using capillary electrophoresis and inverse least squares.

A novel magnetic adsorbent for Pb²⁺ was synthesized based on 4′-aminobenzo-18-crown-6 (AB18C6) and Fe₃O₄–CHO via the Mannich reaction. It showed excellent adsorption and selectivity for Pb²⁺, and the established MSPE method had excellent potential, displaying the selective determination of trace Pb²⁺ from apples, milk and rice.

We designed a near-infrared ratiometric (2.1 to 15.3, I₆₀₈/I₇₃₅) fluorescent probe with a quick response (20 s) based on cyanine polyene derivatives. This probe can detect endogenous hypochlorous acid in HepG-2 cells and determine the hypochlorous acid content in 84 disinfectant and other actual samples.

In this study, voltammetric techniques have been applied to rapidly determine residues of sulfachloropyridazine (SCP) in milk samples.

Efficient resolution of chiral analytes was achieved using chiral stationary phases with chitosan isobutylurea derivatives as the chiral selector.

In this study, a homogeneous fluorescent biosensing strategy is described for the detection of DNA based on an entropy-driven strand displacement reaction and DNAzyme.

In this work, a reduced graphene oxide/Fe₃O₄ nanorod composite (rGO/Fe₃O₄NR) modified glassy carbon electrode was fabricated for nitrofurantoin (NFT) and 1-aminohydantoin (AHD) detection.

An analytical protocol involving microwave assisted solvent extraction followed by GC/MS and principal component analysis (PCA) of the chromatographic data was used for the characterization of bituminous residues sampled from Neolithic objects recovered from excavations in Italy.

The occurrence of endocrine disrupting chemicals (EDCs) in aquatic environments has long been a concern because of their threat to human and aquatic health.

Combination of LIBS and VI-PLS-DA is a promising approach to achieve the online analysis and process control of waste plastic recycling.

An equipment-free and naked-eye biosensor was developed for amplified detection of pesticide acetamiprid using G-quadruplex as the signal reporter.

The NP-ELISA combines traditional nuclease protection with optical and electrochemical enzymatic readout for nucleic acid detection.

In the food industry, fish is a product that has a short shelf life, and xanthine has attracted significant attention as an indicator of the freshness of fish.

Enriching trace amounts of traditional Chinese medicine active components from plasma samples using green and efficient solvents is still challenging.

Enhanced fluorescence sensing of enrofloxacin has been realized by molecular imprinting on curved-surface responsive inverse opal polymers.

Hematocrit increases with storage time, leading to a decrease in transport distance in our paper-based hematocrit device.