A fluorescent probe for the specific detection of cysteine in human serum samples
A turn-on fluorescent probe for cysteine with high selectivity was designed and synthetized. The probe can be used for “naked-eye” detection of Cys with an obvious color change from yellow to colorless. The probe could be applied for Cys detection in real human serum.
A deep-red emission fluorescent probe for detection of viscosity in living cells and mice
A deep-red emission fluorescent probe HY for detecting viscosity in living systems was developed. The probe exhibited ideal properties including good selectivity, high stability and low cytotoxicity. Importantly, the probe HY has been successfully utilized for monitoring viscosity variations in cells and living mice.
Monitoring the trans-membrane transport of single fluorescent silicon nanoparticles based on the force tracing technique
Based on the force tracing technique, the dynamic trans-membrane transport process of single SiNPs in living cells was monitored.
Glycerophospholipids and sphingolipids correlate with poor prognostic genotypes of human papillomavirus in cervical cancer: global lipidomics analysis
Human papillomavirus (HPV), an important causative factor for cervical cancer, alters lipid metabolism.
A non-reductive electrochemical sensor for ultrasensitive detection of pM-level TNT
Most of the current electrochemical sensors for the detection of nitroaromatic explosives directly exploit the reduction of nitro groups at the electrode.
Selective turn-on detection of low levels of cysteine and homocysteine based on fluorescent hydroxyapatite nanoparticles
Fluorescence assay of low levels of biothiols, such as cysteine and homocysteine, based on hydroxyapatite nanoparticles is reported.
Label-free colorimetric aptasensor for highly sensitive and selective detection of proteins by using PNA/DNA hybrids and a cyanine dye
A label-free colorimetric aptasensor that uses PNA/DNA hybrids and a cyanine dye (DiSC2(5)) was developed for highly sensitive and selective detection of proteins in aqueous solution.
Measuring elasticity of wet cellulose fibres with AFM using indentation and a linearized Hertz model
The mechanical properties of different pulp fibres in liquid were measured using an atomic force microscope.
One-step detection of hexokinase activity using a personal glucose meter
Using commercially available personal glucose meters for the determination of hexokinase activity.
An electrostatic self-assembly route to prepare C-dots/gold nanoclusters for dual-emission ratiometric optical thermometry in living cells
A simple and facile self-assemble strategy is developed to prepare C-dots/gold nanoclusters dual-emission ratiometric optical thermometry in living cells.
A simple camphor based AIE fluorescent probe for highly specific and sensitive detection of hydrazine and its application in living cells
The probe DN exhibited excellent selectivity and sensitivity towards ClO−, and it was successfully used for fluorescence imaging of ClO− in living cells.
Re-evaluation assessment of a large volume sampling (LVS) system for the determination of petroleum hydrocarbons and their stable carbon isotopes in deep-sea waters
A large volume sampling technique is used to develop and validate a home-made SPE system connected to a particle collector in situ pump for high sample pre-concentration and determination of petroleum hydrocarbons and their 13C/12C isotope ratio.
Droplet-based microfluidic synthesis of (Au nanorod@Ag)–polyaniline Janus nanoparticles and their application as a surface-enhanced Raman scattering nanosensor for mercury detection
Monodisperse anisotropic (AuNR@Ag)–PANI JNPs were synthesized by using a droplet-based microfluidic platform, and show a fairly good response to Hg2+ ions and have been successfully applied for quantitative analysis of Hg2+ ions in real water samples.
An ultrasensitive label-free photoelectrochemical sensor based on Ag2O-sensitized WO3/TiO2 acicular composite for AFB1 detection
An ultrasensitive label-free photoelectrochemical sensor based on Ag2O-sensitized WO3/TiO2 acicular composite for AFB1 detection.
In situ silanization for continuous stationary phase gradients on particle packed LC columns
This work describes, for the first time, an in situ silanization process for creating phenyl–butyl gradients on particle LC columns.
Highly sensitive and selective colorimetric determination of Staphylococcus aureus via chicken anti-protein A IgY antibody
Utilization of chicken anti-protein A IgY as an antibody pair for sensitive and selective detection of S. aureus.
Comparison of derivatization methods for the quantitative gas chromatographic analysis of oils
Gas chromatographic (GC) determination of quantitative fatty acid composition: which derivatization reagent is the best?
Synthesis of azonia cyanine derivatives as NIR fluorescent probes for nucleic acid detection and cell imaging
A series of azonia cyanine dyes were designed and synthesized for nucleic acid detection and nucleolus imaging in fixed HeLa cells.
Calibration strategies to correct for matrix effects in direct analysis of urine by ICP OES: internal standardization and multi-energy calibration
Internal standardization and MEC can be used successfully to correct for matrix effects in direct analysis of urine by ICP OES.
A novel method based on a Mask R-CNN model for processing dPCR images
The flow of Mask R-CNN model for processing digital polymerase chain reaction (dPCR) fluorescence images.
Rapid quantitative analysis of the acidity of iron ore by the laser-induced breakdown spectroscopy (LIBS) technique coupled with variable importance measures-random forests (VIM-RF)
The LIBS technique coupled with VIM-RF was applied for the rapid analysis of the acidity of iron ore.
Facile synthesis of MoS2 quantum dots as fluorescent probes for sensing of hydroquinone and bioimaging
A simple, low-cost and one-step hydrothermal treatment for the synthesis of molybdenum disulfide quantum dots (MoS2 QDs) is proposed. They exhibit excellent PL properties and can be promising probes for sensing of hydroquinone and bioimaging.
Qualitative discrimination of yeast fermentation stages based on an olfactory visualization sensor system integrated with a pattern recognition algorithm
An olfactory visualization sensor system was developed to verify the feasibility of the in situ monitoring of yeast fermentation stages with a pattern recognition algorithm.
A PET and ESIPT based fluorescent probe for the imaging of hydrogen sulfide (H2S) in live cells and zebrafish
H2S is an important signaling molecule in living systems and participates in a series of physiological and pathological processes.
Development of a fluorescence sensor array for the discrimination of metal ions and brands of packaged water based on gallate-modified polymer dots
An efficient method has been found to discriminate different brands of packaged water which achieves to detect counterfeit products in the packaged water market.
Chemiluminescent sensor for hydrogen sulfide in rat brain microdialysis based on target-induced horseradish peroxidase deactivation
The detection of hydrogen sulfide (H2S) is important due to its role in the diagnosis of many diseases since H2S is involved in the protection of neurons from oxidative stress and neuronal transmission modulation in the brain.
Selective and rapid detection of mercury ion based on DNA assembly and nicking endonuclease-assisted signal amplification
An I-shaped DNA assembly structure coupled with endonuclease-assisted isothermal amplification strategy was constructed for sensitive and fast detection of Hg(II).
A FRET-based ratiometric fluorescent probe for sensing trace water in organic solvents
A new ratiometric fluorescent probe for the detection of water in organic solvents with low limit of detection and large Stokes shift.
Simultaneous determination of cyanide and thiocyanate in swine plasma by high-performance liquid chromatography with fluorescence detection based on a novel D–π–A carbazole-based turn-on fluorescence labeling reagent
The goal of the current work was to develop a simple and sensitive high-performance liquid chromatography in combination with the fluorescence detection (HPLC-FLD) method for the fluorescence turn-on determination of cyanide and thiocyanate in swine plasma.
A two-photon fluorescent probe for imaging of endogenous formaldehyde in HeLa cells and quantitative detection of basal formaldehyde in milk samples
A fluorescent probe NaP which can image endogenous formaldehyde in living cells and quantitatively detect basal formaldehyde in milk samples.
Ag+-coordinated oligonucleotides on gold nanoparticles for anodic-stripping voltammetric immunoassay of cancer antigen 125 for cervical carcinoma
A simple and enzyme-free electrochemical immunoassay was developed for the sensitive screening of cancer antigen 125 using anodic-stripping voltammetry.
A simple strategy for carboxylated MWNTs as a metal-free electrosensor for anchoring the RhB CN group
Organic dye detection requires high sensitivity, low-cost equipment, simplified procedures, and real-time capabilities; thus, electrochemical methods are gradually emerging in this field instead of chromatography.
Fabrication of an antibiotic-sensitive 2D-molecularly imprinted photonic crystal
A 2D-molecularly imprinted colloidal array was fabricated for the visual detection of clindamycin hydrochloride and the LOD is 0.01 mM.
The imaging of local glucose levels in tumor periphery via peroxyoxalate chemiluminescent nanoparticle–glucose oxidase–doped alginate hydrogel
A simple, sensitive, and biological safety POCL–GOx–alginate/Ca gel system to detect and image local concentration of glucose in tumor periphery.
An “on–off–on” fluorescent nanoprobe for recognition of Cu2+ and GSH based on nitrogen co-doped carbon quantum dots, and its logic gate operation
Novel nitrogen co-doped carbon dots (NCDs) were synthesized as a fluorescent “on–off–on” switch for the highly sensitive and selective sensing of Cu2+ and glutathione (GSH) by a straightforward pyrolysis route.
A fluorescent signal-on sensor based on photoinduced electron transfer between copper nanoparticles and G-quadruplex/hemin
A novel fluorescence signal-on biosensing platform was developed by using photoinduced electron transfer (PET) between copper nanoclusters (CuNCs) and G-quadruplex/hemin.
Fiber-optic evanescent wave spectroscopy (FEWS) of crystals from a urine sample as a tool for evaluating the chemical composition of kidney stones
One in every 20 people develops kidney stones at some point in their life.
A turn-on near-infrared fluorescent probe with rapid response and large Stokes shift for the selective and sensitive detection of zinc(II) and its application in living cells
Herein, a new near-infrared turn-on fluorescent probe, YPT, was designed and synthesized for the rapid detection of Zn2+, which exhibited low limit of detection for Zn2+ (12 nM) as well as a favorable large Stokes shift (λem − λex = 168 nm).
A methodology for the fast identification and monitoring of microplastics in environmental samples using random decision forest classifiers
A new yet little understood threat to our ecosystems is microplastics.
Evaluation of glyphosate and AMPA in honey by water extraction followed by ion chromatography mass spectrometry. A pilot monitoring study
Two straightforward methods for glyphosate and AMPA analysis in honey through ion chromatography coupled to Q-Orbitrap accurate high-resolution mass spectrometry as well as their application to real samples are presented.
Indirect competitive ELISA and colloidal gold-based immunochromatographic strip for amantadine detection in animal-derived foods
In this research, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and colloidal gold-based immunochromatographic (GICG) strip were developed for the detection of the antiviral drug amantadine (AM) in animal-derived foods.
A comparison of serum and plasma specimens in NMR-based metabonomics and its application in chronic atrophic gastritis rats
The choice of blood specimens is still disputable in metabonomic studies due to the great metabolic diversities of serum and plasma.
B,N-Co-doped graphene quantum dots as fluorescence sensor for detection of Hg2+ and F− ions
A novel fluorescence sensing platform based on boron and nitrogen co-doped graphene quantum dots (B,N-GQDs) was developed and used to detect the concentration of both Hg2+ and F− ions.
A turn-on near-infrared fluorescent probe for detection of cysteine over glutathione and homocysteine in vivo
Cysteine (Cys) has emerged as a significant sensing target in recent years.
Point-of-care monitoring of intracellular glutathione and serum triglyceride levels using a versatile personal glucose meter
Portable, cost-effective and quantitative detection of various targets related to health at home and in the field has the potential to revolutionize medical diagnostics.
Informatics analysis of capillary electropherograms of autologously doped and undoped blood
Detection of autologous blood doping using capillary electrophoresis and inverse least squares.
4′-Aminobenzo-18-crown-6 functionalized magnetic nanoparticles as a solid-phase extraction adsorbent for the determination of Pb2+
A novel magnetic adsorbent for Pb2+ was synthesized based on 4′-aminobenzo-18-crown-6 (AB18C6) and Fe3O4–CHO via the Mannich reaction. It showed excellent adsorption and selectivity for Pb2+, and the established MSPE method had excellent potential, displaying the selective determination of trace Pb2+ from apples, milk and rice.
A NIR ratiometric fluorescent probe for the ‘naked-eye’ detection of endogenous hypochlorous acid in practical samples
We designed a near-infrared ratiometric (2.1 to 15.3, I608/I735) fluorescent probe with a quick response (20 s) based on cyanine polyene derivatives. This probe can detect endogenous hypochlorous acid in HepG-2 cells and determine the hypochlorous acid content in 84 disinfectant and other actual samples.
Multiwalled carbon nanotube/ionic liquid paste electrode for voltammetric determination of sulfachlorpyridazine
In this study, voltammetric techniques have been applied to rapidly determine residues of sulfachloropyridazine (SCP) in milk samples.
Performance evaluation of enantioseparation materials based on chitosan isobutylurea derivatives
Efficient resolution of chiral analytes was achieved using chiral stationary phases with chitosan isobutylurea derivatives as the chiral selector.
A homogeneous fluorescent biosensing strategy for highly sensitive detection of DNA based on a programmed entropy-driven strand displacement reaction and DNAzyme for dual recycling amplification
In this study, a homogeneous fluorescent biosensing strategy is described for the detection of DNA based on an entropy-driven strand displacement reaction and DNAzyme.
A sensitive electrochemical sensor based on reduced graphene oxide/Fe3O4 nanorod composites for detection of nitrofurantoin and its metabolite
In this work, a reduced graphene oxide/Fe3O4 nanorod composite (rGO/Fe3O4NR) modified glassy carbon electrode was fabricated for nitrofurantoin (NFT) and 1-aminohydantoin (AHD) detection.
Chemical investigations of bitumen from Neolithic archaeological excavations in Italy by GC/MS combined with principal component analysis
An analytical protocol involving microwave assisted solvent extraction followed by GC/MS and principal component analysis (PCA) of the chromatographic data was used for the characterization of bituminous residues sampled from Neolithic objects recovered from excavations in Italy.
Comparison of GC-MS/MS and LC-MS/MS for the analysis of hormones and pesticides in surface waters: advantages and pitfalls
The occurrence of endocrine disrupting chemicals (EDCs) in aquatic environments has long been a concern because of their threat to human and aquatic health.
Rapid classification of plastics by laser-induced breakdown spectroscopy (LIBS) coupled with partial least squares discrimination analysis based on variable importance (VI-PLS-DA)
Combination of LIBS and VI-PLS-DA is a promising approach to achieve the online analysis and process control of waste plastic recycling.
A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity
An equipment-free and naked-eye biosensor was developed for amplified detection of pesticide acetamiprid using G-quadruplex as the signal reporter.
A nuclease protection ELISA assay for colorimetric and electrochemical detection of nucleic acids
The NP-ELISA combines traditional nuclease protection with optical and electrochemical enzymatic readout for nucleic acid detection.
Construction of a novel xanthine biosensor using zinc oxide (ZnO) and the biotemplate method for detection of fish freshness
In the food industry, fish is a product that has a short shelf life, and xanthine has attracted significant attention as an indicator of the freshness of fish.
Enhanced one-step sample pretreatment method for extraction of ginsenosides from rat plasma using tailor-made deep eutectic mixture solvents
Enriching trace amounts of traditional Chinese medicine active components from plasma samples using green and efficient solvents is still challenging.
Enhanced fluorescence detection of enrofloxacin with curved-surface responsive inverse opal polymers and molecular imprinting
Enhanced fluorescence sensing of enrofloxacin has been realized by molecular imprinting on curved-surface responsive inverse opal polymers.
Determination of sample stability for whole blood parameters using formal experimental design
Hematocrit increases with storage time, leading to a decrease in transport distance in our paper-based hematocrit device.
An efficient “Ratiometric” fluorescent chemosensor for the selective detection of Hg2+ ions based on phosphonates: its live cell imaging and molecular keypad lock applications
Herein, we have reported the ability of smart chemosensors (L1 and L2) to selectively detect Hg(II) ions over other metal ions and examined this phenomenon by various spectroscopy techniques.
Simple label-free fluorescence detection of apurinic/apyrimidinic endonuclease 1 activity and its inhibitor using the abasic site-binding fluorophore
A label-free fluorescence detection method for apurinic/apyrimidinic endonuclease 1 activity was reported based on the abasic site-binding fluorophore.
Cysteine-rich protein-templated silver nanoclusters as a fluorometric probe for mercury(II) detection
In this paper, we explored a convenient method for preparing stable silver nanoclusters (AgNCs) within 1 h at room temperature using keratin as a template and NaBH4 as a reducing agent.
Development of a microfluidic device (μPADs) for forensic serological analysis
In this paper, we describe a paper microfluidic device capable of performing a variety of presumptive tests for the presence of biological fluids at crime scenes.
Allele specific DNAzyme assembly for fast and convenient SNP colorimetric genotyping directly from noninvasive crude samples
An allele specific DNAzyme assembly strategy was developed for fast and simple SNP genotyping directly from crude buccal swab samples without DNA extraction and purification.
Characterisation of thin boron-doped diamond films using Raman spectroscopy and chemometrics
A non-destructive chemometric method to determine thickness and boron concentration of as-deposited, thin, heavily doped diamond films by Raman microscopy.
Aluminum-doped NiO nanofibers as chemical sensors for selective and sensitive methanol detection
One-dimensional nanomaterials with controlled morphology have received considerable attention as chemical sensors.
MoS2 QDs co-catalytic Fenton reaction for highly sensitive photoluminescence sensing of H2O2 and glucose
Combing the excellent catalytic and optical properties of MoS2 QDs, the MoS2 QDs-based co-catalytic Fenton system was developed for highly sensitive photoluminescence sensing of H2O2 and glucose.
Two-photon fluorescent probe for hypoxic cancer stem cells by responding to endogenous nitroreductase
A two-photon fluorescent probe to detect the NTR in 3D cultured-cancer stem cells and monitor the hypoxic microenvironment in vitro. This novel probe was hoped to apply for disease-relevant hypoxia detection in the biomedical research fields.