Jump to main content
Jump to site search

Issue 23, 2019
Previous Article Next Article

Simultaneous determination of cyanide and thiocyanate in swine plasma by high-performance liquid chromatography with fluorescence detection based on a novel D–π–A carbazole-based turn-on fluorescence labeling reagent

Author affiliations

Abstract

The goal of the current work was to develop a simple and sensitive high-performance liquid chromatography in combination with the fluorescence detection (HPLC-FLD) method for the fluorescence turn-on determination of cyanide and thiocyanate in swine plasma. For this goal, we designed and synthesized a novel donor–π-bridge–acceptor (D–π–A) carbazole-based fluorescence labeling reagent, 2-(4-carbazol-9-yl-benzylidene)-malononitrile (CBM), to label cyanide and thiocyanate. Cyanide and thiocyanate can be rapidly labeled by CBM within only 5 min at room temperature (25 °C). Attractively, CBM itself has no fluorescence, while its cyanide and thiocyanate derivatives expressed intense fluorescence with the maximum excitation and emission wavelengths of 290 nm and 380 nm, which extraordinarily diminished the background interference and improved the detection sensitivity of cyanide and thiocyanate. The labeled derivatives were analyzed on a reversed-phase Eclipse XDB-C8 column within 8 min. The excellent linearities (R2 > 0.996) of cyanide and thiocyanate were achieved with the limits of detection (LODs) and the limits of quantitation (LOQs) in the low micrograms per litre levels of 0.25 and 0.30 μg L−1, 0.80 and 1.00 μg L−1, respectively. Using the proposed HPLC-FLD method, we successfully determined the endogenous cyanide and thiocyanate in swine plasma with good applicability. Furthermore, the developed sensitive and reliable method could also be used for the determination of cyanide and thiocyanate in other samples.

Graphical abstract: Simultaneous determination of cyanide and thiocyanate in swine plasma by high-performance liquid chromatography with fluorescence detection based on a novel D–π–A carbazole-based turn-on fluorescence labeling reagent

Back to tab navigation

Supplementary files

Article information


Submitted
14 Apr 2019
Accepted
13 May 2019
First published
22 May 2019

Anal. Methods, 2019,11, 2983-2990
Article type
Paper

Simultaneous determination of cyanide and thiocyanate in swine plasma by high-performance liquid chromatography with fluorescence detection based on a novel D–π–A carbazole-based turn-on fluorescence labeling reagent

P. Luo, Y. Yu, D. Wu, X. Li, C. Dai, X. Chen, G. Li and Y. Wu, Anal. Methods, 2019, 11, 2983
DOI: 10.1039/C9AY00784A

Social activity

Search articles by author

Spotlight

Advertisements