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Label-Free Colorimetric Aptasensor for Highly Sensitive and Selective Detection of Protein by Using PNA/DNA Hybrids and a Cyanine Dye

Abstract

Economical, fast and sensitive detection of proteins is essential to biomedical research as well as clinical diagnosis. Here, we demonstrated a label-free colorimetric aptasensor that uses PNA/DNA hybrids and a cyanine dye (DiSC2(5)) for highly sensitive and selective detection of protein in aqueous solution. This protocol, based on the difference in the binding interactions of DiSC2(5) with PNA and PNA/DNA hybrid causing different colors, could be applied for the detection of proteins. Using thrombin as an example analyte, this sensing platform can detect thrombin specifically with a detection limit of 0.4 nM. Moreover, the assay does not involve any chemical modification and enzyme amplification, which is convenient, fast and low-cost. In principle, this approach can be used to detect proteins with specific aptamers by simply changing the sequences of the probe DNA to a specific target. Furthermore, this sensing platform provides a promising approach for the analysis of target proteins at point-of-care in resource-limited settings.

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Supplementary files

Publication details

The article was received on 12 May 2018, accepted on 10 Jul 2018 and first published on 11 Jul 2018


Article type: Communication
DOI: 10.1039/C8AY01071D
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Label-Free Colorimetric Aptasensor for Highly Sensitive and Selective Detection of Protein by Using PNA/DNA Hybrids and a Cyanine Dye

    M. Xu, S. Xing, X. Xu, P. Fu, W. Xu and C. Zhao, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY01071D

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