Themed collection Clinical spectroscopy

Welcome to the latest themed issue on the topic of Optical Diagnosis in Analyst, guest-edited by Dr. Matthew Baker.

Welcome to the latest themed issue on the topic of Optical Diagnosis in Analyst, guest-edited by Kathleen Gough and Frédéric Leblond.

Quantum cascade lasers with high spectral power density and narrow band emission profiles provide novel access to biomedical applications.

This review examines the state-of-the-art of clinical applications of infrared absorption and Raman spectroscopy, outstanding challenges, and progress towards translation.

This review summarizes actual clinical needs in oncology that can be addressed by Raman spectroscopy, provides results published in the last 10 years and analyses the current status of translation of these results into clinical practice.

This review explores Raman technologies with a particular emphasis on their utility for cancer diagnostic applications.

This review takes a fresh approach from the patient perspective; offering insight into the applications of midinfrared biomedical spectroscopy.

A way to incorporate SERS nanosensors on the end of an optical fibre that also allows for the extraction of bacterial samples.

First IR-SNOM images over the Amide I band taken in the centre of an oesophageal cancer cell.

Identification of bacterial species is a crucial bottleneck for clinical diagnosis of infectious diseases.

Variable angle micro ATR-FTIR, via the insertion of circular apertures, was used to measure tissue samples at various penetration depths.

Radiobiological effects occurring in normal human breast cells exposed to a low dose of a clinical proton beam are detectable by means of Raman spectra and the ratiometric analysis of Raman peak intensities.

A specific reaction-based SERS approach was developed for the selective and sensitive detection of leucine aminopeptidase activity in serum.

Raman studies of dynamically expanded cell-free plasma domains in microfluidic blood flow.

A novel photosensitiser, DC473, designed with solvatochromatic fluorescence and distinct Raman signal, is detected with tandem fluoRaman in SW480 cells.

Inflammatory breast cancer (IBC) has a poor prognosis because of the lack of specific biomarkers and its late diagnosis.

Non-negative factorization analysis applied to spontaneous Brillouin microscopy maps of amyloid-beta plaques in a transgenic mouse model enables to resolve spatially distinct components with specific mechanical properties.

Pre-processing is an essential step in the analysis of spectral data.

This study demonstrates the efficacy and distribution of sorafenib in colon cancer cells by label-free Raman microscopy.

Binding sites of albumins on gold nanoparticles were characterized by surface-enhanced Raman scattering.

Gaining knowledge on the biochemical profile of primary endothelial cells on a subcellular level can contribute to better understanding of cardiovascular disease.

ATR-FTIR spectroscopy has been applied to compare the effect of new organoamidoplatinum(II) complexes with cisplatin on cells from a cisplatin-sensitive and a cisplatin-resistant ovarian cancer cell line.

Translating Raman spectroscopy for colorectal cancer diagnosis with a focus on high-throughput design, inter-user variability and sample handling considerations.

A probe-based Raman spectroscopy system using a single laser module with two excitation wavelengths has been developed for measuring the fingerprint and high wavenumber regions with a single detector.

Oral injuries are currently diagnosed by histopathological analysis of biopsy, which is an invasive procedure and does not give immediate results.

Spectral information relevant to the quality of stored blood can be obtained in situ through sealed blood transfusion bags using a commercially available instrument.

First near-field infrared spectroscopy and imaging of lymphocyte nucleus at 30 nm spatial resolution reveals spectrochemically distinct regions in nuclear organization.

This study explores the potential of Raman spectroscopy, coupled with multivariate regression techniques and ion exchange chromatography, to quantitatively monitor diagnostically relevant changes in high molecular weight proteins in liquid plasma.

One of the new targets of untapped therapeutic potential is perivascular adipose tissue (pVAT). Based on Raman spectroscopy we demonstrated that the lipid unsaturation degree was clearly distinct in various types of adipose tissues, in particular thoracic and abdominal pVATs, and was influenced by the age of animals.

The investigation of blood plasma via¹H NMR metabolomics revealed a panel of potential biomarkers of pancreatic cancer.

PCA score of FTIR spectra in fingerprint region for cultured chondrocytes at day 7, 14 and 21 in HA hydrogel showing that the 3D culture drove chondrocytes (CY) to chondron-like (CN) structure dynamically.

Through tissue imaging of a live breast cancer tumour model using handheld surface enhanced resonance Raman spectroscopy (SERRS).

Plasma samples deposited on low-E slides were analysed in transmission mode by using a FT-NIR spectrometer in order to detect Alzheimer's disease using computer-based methods.

A method to acquire the Raman spectra of sub-surface components using diffusely focused radiation in a microscope sampling configuration is described.

Raman spectroscopy proved why the antibacterial response of non-oxide Si₃N₄ bioceramic is superior to those of alumina-based oxide bioceramics.

The SERS spectra of human urine could provide valuable information for the noninvasive and prospective diagnosis of patients with suspected CHD.

Serum Raman spectroscopy is explored to classify meningiomas, which are the most frequently reported non-malignant brain and CNS tumors and are often misdiagnosed. We find the technique can be prospectively extended to the screening of high-risk populations and for therapeutic monitoring.

Chronic venous leg ulcer arises as a venous insufficiency complication and is a cause of great morbidity.

Cellular macromolecules play important roles in cellular behaviors and biological processes.

Augmenting tissue histology with spectral information from mid-IR spectroscopy will benefit a lot. Optimal bands selection needed to do histopathological classification of mid-IR images will help to reduce imaging time and computation complexity. GPU use for feature selection makes it practical.

Determining E. coli bacteria susceptibility by analyzing their FTIR spectra using multivariate analysis.

We report the development of a new technique to screen protein aggregation based on laser-probing spectroscopy with sub-picosecond resolution.

Mid-infrared microscopy has become a key technique in the field of biomedical science and spectroscopy. In this current study, we explore the use of a QCL infrared microscope to produce high definition, high throughput chemical images useful for the screening of biopsied colorectal tissue.

High throughput Raman spectroscopy method for rapid and accurate diagnosis of prostate cancer using liquid plasma samples.

Non-destructive near infrared spectroscopic data can be utilized for assessment of compositional and mechanical properties of engineered cartilage.

Monitoring of changes in the concentrations of the low molecular weight constituents enhanced by abundant proteins depletion.

There has recently been significant interest within the vibrational spectroscopy community to apply quantitative spectroscopic imaging techniques to histology and clinical diagnosis.

A simple blood test for the diagnosis of Alzheimer's disease using FTIR microscopy.

The great capability of virtual staining for label-free classification of colon cancer tissue has been demonstrated via Raman spectral imaging.

The diagnostic performance of Raman spectroscopy for differentiating lichen sclerosus from other vulval conditions in fresh vulval biopsies is demonstrated.

A filter system to perform in vivo Raman spectroscopy measurements under microscope lighting for seamless integration into the surgical workflow.

Spectroscopic diagnostics have been shown to be an effective tool for the analysis and discrimination of disease states from human tissue.

This method utilizes machine learning algorithms and fluid mechanic properties of cells in a microfluidic channel to find the compositions of cell mixtures.

Fourier transform infrared (FTIR) spectroscopy has long been established as an analytical technique for the measurement of vibrational modes of molecular systems.

An immunoassay protocol is described to detect tumor markers in human serum based on a sandwich structure consisting of nano-Si immune probes and SiC@Ag SERS-active immune substrate.

One strategy to improve the clinical outcome of radiotherapy is to use nanoparticles as radiosensitizers.

Biothiols are determined in whole blood samples by Surface Enhanced Raman Spectroscopy (SERS).

Spatially Offset Raman spectroscopy (SORS) has been used to retrieve biochemical information from red blood cell concentrate within sealed PVC bags, demonstrating the potential of SORS to non-invasively assess the condition of the cells prior to transfusion.

Sensing of methotrexate at clinically-relevant concentrations was achieved with a plasmon-coupling assay.

Direct growth of CNTs on 3D microelectrodes could detect distinguished zebrafish ECG resulting from the interfacial improvement analyzed by EIS.