Chemical methods for modification of proteins
The field of protein bioconjugation draws attention from stakeholders in chemistry, biology, and medicine. This review provides an overview of the present status, challenges, and opportunities for organic chemists.
Red-shifted backbone N–H photocaging agents
A 3-nitrodibenzofuran cure provides blue-shifted reactivity in vinylogous photocleavage processes.
Efficient and selective antibody modification with functionalised divinyltriazines
We have developed a highly efficient disulfide rebridging strategy for the modification of antibodies with substituted divinyltriazine linkers.
Disulphide-mediated site-directed modification of proteins
Site-directed addition of a single thiols handle to proteins by means of temporary disulphide rebridging of solvent exposed disulphides is obtained with a new labelling reagent.
Site-selective modification of proteins using cucurbituril as supramolecular protection for N-terminal aromatic amino acids
Supramolecular protection of N-terminal aromatic amino acids through complexation with cucurbituril can enable site-selective protein modification of unfavored motifs.
Rapid sodium periodate cleavage of an unnatural amino acid enables unmasking of a highly reactive α-oxo aldehyde for protein bioconjugation
A genetically incorporated ThrK unnatural amino acid can undergo rapid periodate oxidation to reveal a reactive internal α-oxo aldehyde.
A laccase-catalysed tyrosine click reaction
The tyrosine click reaction of peptides/proteins with the tyrosine modification reagent, N-methyl luminol, was catalysed by a laccase in the presence of molecular oxygen (O2) at 37 °C.
Selective modification of sulfamidate-containing peptides
Hybrid peptides whose N-terminal residues are activated in the form of α-methylisoserine cyclic sulfamidates exhibit rich reactivity as electrophiles, allowing site- and stereoselective modifications at different backbone and side chain positions.
About this collection
The chemical modification of proteins is an important and ever growing field. This collection, guest edited by Professors Annemieke Madder (Ghent University, Belgium), Gonçalo Bernardes (University of Cambridge, UK), and Hiroyuki Nakamura (Tokyo institute of Technology, Japan) showcases the latest advances in Methodology Development for Protein Modifications. The collection scope covers but is not limited to all aspects of new synthetic methods towards protein modification and their application.
New articles will be added to the collection upon publication. Please return to this page frequently to see the collection grow.