Themed collection Analyst HOT Articles 2024

This review summarizes sensitive analytical detection. Analyte pre-concentration by droplet deposition is combined with SERS enhancement.

This minireview summarizes the recent advancements in impact electrochemistry-based biosensing over the past two to three years and provides insights into the future directions of this field.

This review aims to introduce the development of DNA nanotechnology and recent advances of DNA nanostructure-based delivery systems for bioimaging, disease diagnosis, and therapeutic applications.

Surface-enhanced Raman scattering (SERS) is a very important tool in vibrational spectroscopy.

The research progress of MOF-based nanozymes with oxidoreductase-like activity.

The abnormal expression of human DNA methyltransferases (DNMTs) is closely related with the occurrence and development of a wide range of human cancers.

Photoactivated fluorophores (PAFs) are highly effective imaging tools that exhibit a removal of caging groups upon light excitation, resulting in the restoration of their bright fluorescence.

Patients with end-stage kidney disease (ESKD) rely on dialysis to remove toxins and stay alive.

Overview of recent studies on SERS for biomolecular complexities analysis.

Different purification techniques for carbon dots.

Overview of nanopores for single cell detection.

This review summarizes the recent advances in three-dimensional graphene structures, including preparation methods and their detection application in the biomedical field.

Synthesis, characterization, and application of carbon dots@noble metal nanocomposites.

Fluorometric, colorimetry, chemiluminescence, SERS, and dual-mode techniques for biosensing and imaging of telomerase are summerized. Relevant signal amplification strategies are included to provide ideas for exploration of more practicable methods.

Herein the development of cellular impedance biosensors, electrochemical impedance spectroscopy, and the general principles and terms associated with the cell–electrode interface is reviewed.

Seeing is believing - and optical sensors (optodes) are ideal tools to make chemistry visible in real time. Find out how technological and chemical advances enable multiparameter imaging of complex samples.

Visual electrochemiluminescence (ECL) emission from L012 and hydrogen peroxide is generated from an all-solid-state electrochemical cell with a polyacrylamide hydrogel as the solid electrolyte.

The photovoltage (V_oc) of n-Si/Au photoelectrodes varies with the solution potential (E⁰) when E⁰ falls between the valence and conduction band edges of the semiconductor but is constant when E⁰ is more positive than the valence band edge.

A novel green and eco-friendly carbon-based nanoprobe was developed for dual-channel analysis of tartrazine (Trz) and Pd(II) involving, respectively, fluorescence resonance energy transfer (FRET) and electron transfer mechanisms.

We report here a tunable proton platform for the tumor micro-acid environment that is used for tumor imaging and treatment.

Pure shift NMR experiments greatly enhance spectral resolution by collapsing multiplet structures into singlets and, with optimal water suppression, can be used for aqueous samples.

Enzymes (1) and nanozymes (2) have significant differences in the Michaelis–Menten constant. Detection of nanozymes using enzyme substrates results in lower catalytic activity and a significantly higher limit of detection.

Graphene oxide can increase the levels of intracellular H₂O₂ and ˙OH, and cause the irreversible oxidative modification. This leads to the activation of the JNK/p38 signaling pathway and promote hepatocyte apoptosis.

A split G-quadruplex based PfAgo sensing platform was developed for nucleotide mutation discrimination and human genotyping.

The development of an in situ combination of AFM and soft X-ray μXRF in a single instrument, with AFM integration accomplished without substantial original set-up alteration.

An electrochemical sensing approach for ultrasensitive MTase activity assay is proposed based on cascade strand displacement polymerization after specific cleavage reaction in the methylated state.

Our dual-mode FI and FP aptamer probe detects P-glycoprotein in drug-resistant ovarian cancer cells, demonstrating high specificity and sensitivity with detection limits of 1.13 nM and 40 cells per mL, promising improved diagnosis strategies.

We quantified the dissolution rate of sub-nL oil microdroplets in a bulk aqueous phase based on the evolution of cyclic voltammograms recorded for a redox mediation confined in the microdroplet.

An electrochemical aptamer biosensor based on the CRISPR-Cas12a system was constructed for the detection of cancer tumor biomarker MUC1 mucin, the sensitivity was significantly improved by employing the enzyme catalytic reaction.

Combining target-initiated 3D DNAzyme motors with single-molecule imaging enables intuitive, sensitive and rapid detection of different miRNAs. Dual miRNA detection of EVs using this method is effective for clinical diagnosis of colorectal cancer.

A periodic mesoporous organosilica (PMO)-based heteromembrane with hierarchical pore architecture and glutathione (GSH)-responsive switchable wettability is synthesized for sensitive and efficient GSH detection.

Development of an acoustic methodology for the sensitive detection of LCR-amplified cancer mutations at a single point mutation-level.

A novel nanocomposite, [Eu(BTD)₃(DPBT)]–BSA@MnO₂, is reported to serve as an effective nanoprobe for bimodal time-gated luminescence (TGL) and magnetic resonance (MR) imaging of H₂O₂in vitro and in vivo.

Fabrication of chemically synthesised silica polyethylene nanoparticles acted as an all-in-one solid-state ECL platform for selective sensing of spermine which offers an expediting the miniaturization of sensing devices and point-of-care needs.

We developed a simple and effective electrochemical signal amplification strategy for intracellular ATP quantification, which replaces the regular ATP aptamer-linked ferrocene monomer to a polymer on electrochemical nanosensors.

Latent fingermarks on non-conducting surfaces such as glass, ceramics, and plastics can be visualized electrochromically using benzyl viologen and with the assistance of gold layer.

An organ-on-a-chip in vitro human model of the blood–brain barrier (BBB) was applied to iteratively enrich for AAV variants that efficiently cross the BBB and transduce astrocyte cells.

A non-invasive, label-free and high-precision diagnosis of Helicobacter pylori (H. pylori) infection is conducted by using a dual-element multimodal gas sensor array.

New fluorogenic indicators for HIV-1 TAR RNA have been developed for fluorescence indicator displacement (FID) assay based on forced intercalation of thiazole orange unit integrated into the middle of transactivator of transcription (Tat) peptide.

An LC-MS/MS-based assay for monitoring sphingomyelin synthase (SMS) activity was developed. The method is highly useful in screening novel inhibitors for SMS.

PolyA-TDF enables PCR-free qualitative detection of drug-resistant genes, integrating biosensor recognition and probe immobilization via strong adsorption to gold.

In this work, the release of giant liposome (∼100 μm in diameter) content was imaged by shadow electrochemiluminescence (ECL) microscopy.

An electrochemical microsensor of mesothelin was prepared based on surface imprinted nano-interface coordinated embedded thionine, and it was constructed via a green route of electrosynthesis and self-assembly.

A magneto-electrochemical method for enhanced chiral recognition of amino acid enantiomers has been developed.

The finte element simulation predicts that interactions of a biological membrane with a permeant can be determined quantitatively by employing transient scanning electrochemical microscopy to discriminate between one-step and two-step transport.

Here, we report a proof-of-concept resistive pulse method for analyzing chiral amino acids utilizing metal–amino acid crystallization differences.

We construct a hybrid photonic–plasmonic resonator SERS substrate with high enhancement factor and can be used to detect cancer biomarkers in serum for differentiation of different cancers.

Anisotropic growth of gold anchors on CdSe semiconductor quantum platelets for self-assembled architectures with well-connected electronic circuits for enrofloxacin electro-oxidation.

A binding-triggered hybridization chain reaction cascade multi-site activated CRISPR/Cas12a signal amplification strategy for sensitive detection of α-synuclein. This work provides a promising analysis tool for the early diagnosis of Parkinson's disease.

For the first time, a novel strategy for fabrication of maintenance-free and contamination-free polymeric membrane potentiometric sensors is proposed.

The upper is a schematic of the swelling kinetics of a single representative PMMA microsphere in solvent at different reaction time nodes. The bottom shows the corresponding optical tracking of the extraction process of edge changes during the swelling of a single PMMA particle.

Sensing and visualization of metabolites and metabolic pathways in situ are significant requirements for tracking their spatiotemporal dynamics in a non-destructive manner.

A spatially resolved optical method was developed to measure oxygen consumption rate (OCR) in ex vivo tissue slices. This method revealed regional OCR variations and is a valuable tool for exploring tissue hypoxia dynamics and immune cell activity.

Direct analysis of patient urine by paper spray mass spectrometry combined with machine learning has the potential to be used as a rapid and simple diagnostic tool for chronic kidney disease.

A novel cell tracking algorithm, named HFM-Tracker (Hybrid Feature Matching Tracker), is proposed to accurately track the migratory behavior of cells through the capture of time-lapse cell images.

A BFC-based electrochemical aptasensor for E2 detection based on CNCs/AuNPs and PMNPs@aptamer DNA bioconjugate.

An electrochemical biosensor for fast and sensitive Staphylococcus aureus detection is achieved by using surface multilevel 3D micro/nanostructures.

A schematic diagram of RAA-CRISPR/Cas12a-FQ and RAA-CRISPR/Cas12a-FB methods in NiV detection.

CID fragmentation of the synthesized [B₁₂I₁₁(SCN)]²⁻ ions yields [B₁₂I₈S(CN)]⁻ ions. The reactivity of these ions was studied in the gas phase and in ion soft-landing experiments, where selectivity toward certain functional groups was discovered.

A new method utilizing fluorescent ratiometry is proposed for detecting putrescine and spermidine.

Laser-heated electrospray ionization with mass spectrometry enables melting temperature measurements of aggregation-prone proteins from which thermochemical and mechanistic information about protein unfolding and ligand loss is deduced.

Building a high-performance sensing platform is the key to developing sensitive sensors.

A novel near-infrared fluorescent (NIRF) and photoacoustic (PA) dual-modal probe with enhanced sensitivity and a high PA signal-to-noise ratio was constructed for imaging HNO in vivo.

This study presents a photoelectrochemical sensing platform for nucleic acid detection based on the on the varying protective effects of single-stranded DNA of different lengths against silver nanoparticles.

A convergent sector in microfluidic devices utilizing ion concentration polarization (ICP) can help increase the preconcentration rate and the concentration enhancement factor (CEF) of biomolecules.

The fabricated NiO/Ti₃C₂T_x sensor exhibits a high response value of 950% to 50 ppm TEA with fast response/recovery times of 8/40 s.

An alternative to serial NMR measurement on a benchtop machine exploits a pH gradient along the NMR tube and mechanical sample shifting. This approach allows one to detect and characterize “pH-sensitive” peaks.