Issue 8, 2024

Characterization of nanozyme kinetics for highly sensitive detection

Abstract

Nanozymes have been widely used as enzyme substitutes. Based on a comprehensive literature survey of 261 publications, we report the significant differences in the Michaelis–Menten constants (Km) between peroxidase-mimicking nanozymes and horseradish peroxidase (HRP). Further, these differences were not considered in more than 60% of the publications for analytical developments. As a result, nanozymes’ catalytic activity is limited, resulting in a potentially higher limit of detection (LOD). We used a peroxidase-mimicking Au@Pt nanozyme, which has Km for TMB comparable with HRP and three orders of magnitude higher Km for H2O2. Using the Au@Pt nanozyme as a label for immunoassays, non-optimized nanozyme substrate concentrations led to 30 times higher LOD compared to optimized conditions. The results confirm the necessity of measuring nanozymes’ kinetic parameters and the corresponding adjustment of substrate concentrations for highly sensitive detection.

Graphical abstract: Characterization of nanozyme kinetics for highly sensitive detection

Supplementary files

Article information

Article type
Communication
Submitted
16 Feb 2024
Accepted
14 Mar 2024
First published
20 Mar 2024

Analyst, 2024,149, 2223-2226

Characterization of nanozyme kinetics for highly sensitive detection

V. G. Panferov, X. Wang and J. Liu, Analyst, 2024, 149, 2223 DOI: 10.1039/D4AN00250D

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