Graphene field-effect transistors as bioanalytical sensors: design, operation and performance
Changes in the electrical conductance of graphene field-effect transistors (GFETs) are used to perform quantitative analyses of biologically-relevant molecules such as DNA, proteins, ions and small molecules.
Ion-selective polymer dots for photoelectrochemical detection of potassium ions
Potassium-selective polymer dots (K-Pdots) containing potassium ionophores were for the first time used for photoelectrochemical (PEC) analysis and yielded sensitive and specific detection of potassium ions.
Quantifying cyanide in water and foodstuff using corrin-based CyanoKit technologies and a smartphone
This paper describes the detection of endogenous cyanide using corrin-based CyanoKit technologies in combination with a smartphone readout device.
Paper-based SERS analysis with smartphones as Raman spectral analyzers
We present a smartphone-based portable Raman spectrometer, which has a friendly human–machine interface, easy operation, rapid response time, and most importantly a very small size for on-site use.
Lectin inspired polymers based on the dipeptide Ser-Asp for glycopeptide enrichment
Lectin inspired polymers polySD-SiO2 were prepared and applied to the high-efficiency enrichment of glycopeptides.
Detection of Escherichia coli bacteria by impact electrochemistry
Employing the redox species N,N,N′,N′-tetramethyl-para-phenylene-diamine (TMPD), the label-free detection of E. coli, based on an electrochemical “on”-signal during impact electrochemistry, is reported for the first time.
A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering
We develop a ratiometric Raman probe for visualizing hydrogen sulfide in living cells as the first alkyne-based sensor for SRS microscopy.
In vivo detection of drug-induced apoptosis in tumors using Raman spectroscopy
We describe a label-free approach based on Raman spectroscopy, to study drug-induced apoptosis in vivo.
Highly sensitive and on-site NO2 SERS sensors operated under ambient conditions
This paper presents the first demonstration of highly sensitive and on-site SERS-based NO2 sensors with a detection limit of 0.1 ppm operated under ambient conditions.
A new enzyme immunoassay for alpha-fetoprotein in a separate setup coupling an aluminium/Prussian blue-based self-powered electrochromic display with a digital multimeter readout
A new immunoassay was designed for the detection of disease-related biomarkers (alpha-fetoprotein, AFP, as a model), coupling an aluminium (Al)/Prussian blue-based electrochromic display with a digital multimeter readout.
Wettability alteration in a functional capillary tube for visual quantitative point of care testing
A quantified POCT device was designed based on wettability alteration in a capillary tube.
A novel electrochemiluminescence biosensor based on S-doped yttrium oxide ultrathin nanosheets for the detection of anti-Dig antibodies
The mechanism of a novel electrochemiluminescence biosensor based on S-doped yttrium oxide ultrathin nanosheets for detection of anti-Dig antibodies.
Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction
We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Small molecule–protein interactions in branch migration thermodynamics: modelling and application in the homogeneous detection of proteins and small molecules
A homogeneous assay for proteins and small molecules with ultra-high inhibition factors and sensitivity was established.
A thumb-size electrochemical system for portable sensors
A thumb-size and precise electrochemical system is designed for sensors in size and cost sensitive applications.
An ultrasensitive photoelectrochemical bioanalysis strategy for tumor markers based on the significantly enhanced signal of a bismuth oxyiodine microsphere/graphitic carbon nitride composite
An ultrasensitive PEC bioanalysis strategy was designed based on the significantly enhanced signal of BiOI/g-C3N4 and amplified signal variation of CuS NPs.
Time-resolved visual detection of heparin by accelerated etching of gold nanorods
Plasmonic gold nanorods are promising and sensitive light scattering probes, which can reach the single particle level. Herein, we present the scattering properties of gold nanorods for time-resolved visual detection of heparin based on the rapid etching of gold nanorods under dark-field microscopy.
Deducing disulfide patterns of cysteine-rich proteins using signature fragments produced by top-down mass spectrometry
With improved deconvolution and fragment identification strategies, we use the patterns of specific modifications of fragment ions resulted from disulfide cleavage in top-down mass spectrometry to deduce the complex disulfide patterns of cysteine-rich proteins.
A Portable SERS Reader Coupled with Catalytic Hairpin Assembly for Sensitive MicroRNA-21 Lateral Flow Sensing
A DNA-functionalized biomass nanoprobe for targeted photodynamic therapy of tumor and ratiometric fluorescence imaging-based visual cancer cell identification/antitumor drug screening
A turn-on near-infrared fluorescent probe for visualization of endogenous alkaline phosphatase activity in living cells and zebrafish
Alkaline phosphatase (ALP) is an essential hydrolase and widely distributed in living organisms.
Ratiometric electrochemical sensing based on Mo2C for detection of acetaminophen
Schematic presentation of a novel ratiometric electrochemical sensor based on a Mo2C modified glassy carbon electrode for the determination of AP.
A micro passive preconcentrator for micro gas chromatography
We demonstrate “zero-power” sampling at known rates for up to 24 h, high capacity, and high desorption (transfer) efficiency for downstream separation and detection.
A novel electrochemiluminescence sensor based on resonance energy transfer from MoS2QDs@g-C3N4 to NH2-SiO2@PTCA for glutathione assay
A novel “off–on” ECL GSH sensor based on RET from MoS2QDs@g-C3N4 to NH2-SiO2@PTCA.
Dual-recognition-based determination of ctDNA via the clamping function of peptide nucleic acid and terminal protection of small-molecule-linked DNA
A new dual-recognition fluorescent biosensor for circulating tumor DNA (ctDNA) detection has been developed, which combines the clamping function of peptide nucleic acid (PNA) and terminal protection of small-molecule-linked DNA (TPSMLD).
Quantitative and sensitive detection of lipase using a liquid crystal microfiber biosensor based on the whispering-gallery mode
We demonstrate a quantitative and sensitive strategy for monitoring the lipase concentration using a liquid crystal microfiber biosensor based on the whispering-gallery mode.
A versatile magnetic beads-based flow cytometric assay for detection of thyroid cancer related hsa-miR-221-3p in blood and tissue
Microfluidic filter device coupled mass spectrometry for rapid bacterial antimicrobial resistance analysis
Microfluidic chip and electrospray ionization mass spectrometry based bacterial antimicrobial resistance analysis has been implemented rapidly and accurately.
A two-dimensional photonic crystal hydrogel biosensor for colorimetric detection of penicillin G and penicillinase inhibitors
A simple 2DPPCH biosensor was developed for colorimetric detection of penicillin G and penicillinase inhibitors based on the enzymatic hydrolysis reaction between penicillin G and penicillinase.
Bottom-up and top-down profiling of pentosan polysulfate
Pentosan polysulfate (PPS), a semi-synthetic glycosaminoglycan (GAG) mimetic, is profiled by bottom-up and top-down analysis.
Study of porphyrin-modified liquid exfoliated graphene field-effect transistors for evaluating DNA methylation degree
Recognizing and counting 5-methylcytosine (5mC) sites on ssDNA are realized by porphyrin-modified liquid exfoliated graphene field-effect transistors (TAPP@LEG-FETs).
A semiconductor quantum dot-based ratiometric electrochemical aptasensor for the selective and reliable determination of aflatoxin B1
A ratiometric electrochemical aptasensor was developed for the selective and reliable determination of aflatoxin B1 with an inherent reference signal.
A near-infrared biothiol-specific fluorescent probe for cancer cell recognition
A novel near-infrared biothiol-specific fluorescent probe can discriminate cancer cells from normal cells showing great promise for cancer diagnosis.
Postsynthetic functionalization of water stable zirconium metal organic frameworks for high performance copper removal
Thiol functionalized water stable zirconium MOFs were synthesized and used for copper adsorption with enhanced adsorption capacity and stability.
A water-stable La-MOF with high fluorescence sensing and supercapacitive performances
La-MOF shows excellent chemical stability and high fluorescence sensing. La-MOF electrode exhibits high specific capacity and good cycling stability.
High-affinity Fe3O4/Au probe with synergetic effect of surface plasmon resonance and charge transfer enabling improved SERS sensing of dopamine in biofluids
Fe3O4/Au composites demonstrated a coupled enhanced mechanism allowing for sensitive detection of dopamine in complicated specimens subjected to simple pretreatment.
Modulating an in situ fluorogenic reaction for the label-free ratiometric detection of biothiols
A label-free ratiometric method for biothiol detection is developed based on the modulation of fluorogenic reaction and the inner filter effect.
A dual-responsive luminescent metal–organic framework as a recyclable luminescent probe for the highly effective detection of pyrophosphate and nitrofurantoin
Luminescent ZTMOF-1 can discriminately detect PPi and NFT with high selectivity, sensitivity and stability.
Fluorescent visual quantitation of cell-secreted sialoglycoconjugates by chemoselective recognition and hybridization chain reaction
A fluorescent visual method is developed for the quantitation of cell-secreted sialoglycoconjugates by chemoselective recognition and hybridization chain reaction.
A simple highly specific fluorescent probe for simultaneous discrimination of cysteine/homocysteine and glutathione/hydrogen sulfide in living cells and zebrafish using two separated fluorescence channels under single wavelength excitation
A simple highly specific fluorescent probe was reasonably constructed to simultaneously detect Cys/Hcy and GSH/H2S in living cells and zebrafish using two separated fluorescence channels under single wavelength excitation.
Advances in age-dating of individual uranium particles by large geometry secondary ion mass spectrometry
Age-dating measurements are performed on individual uranium particles that are younger, smaller, and less enriched in 235U than previously reported.
Catalase-linked immunosorbent pressure assay for portable quantitative analysis
In this study, catalase-linked immunosorbent pressure assay with a gas-generation reaction was established for quantitative detection of disease biomarker C-reactive protein (CRP) by a portable pressuremeter.
Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals
Mesoporous silica functionalised with a cyclic spin trap enabled the identification of a wide range of radicals in organic and aqueous media, including superoxide radical anion.
Microfluidic co-culture of liver tumor spheroids with stellate cells for the investigation of drug resistance and intercellular interactions
Hepatic stellate cells (HSCs), a major component of the tumor microenvironment in liver cancer, play important roles in cancer progression as well as drug resistance.
A dual-mode fluorometric/colorimetric sensor for Cu2+ detection based on hybridized carbon dots and gold–silver core–shell nanoparticles
A fluorometric and colorimetric dual mode sensing platform based on hybridized carbon dots (Cdots) and gold–silver core–shell nanoparticles (Au@Ag NPs) has been established for the sensitive detection of trace Cu2+ ions in aqueous solution.
Multi-code magnetic beads based on DNAzyme-mediated double-cycling amplification for a point-of-care assay of telomerase activity
Development of a reliable and facile telomerase activity assay with high specificity and sensitivity is a central challenge to make telomerase testing a routine part of medical care with respect to cancer.
Electronically-tuned triarylmethine scaffolds for fast and continuous monitoring of H2S levels in biological samples
A fast and reliable spectrophotometric method for the selective detection and quantification of H2S in biological samples is presented.
Volatomic pattern of breast cancer and cancer-free tissues as a powerful strategy to identify potential biomarkers
Breast cancer (BC), ranked as the fifth amongst all cancers, remains at the top of women's cancers worldwide.
Two-photon imaging of the endoplasmic reticulum thiol flux in the brains of mice with depression phenotypes
Two-photon fluorescent probe ER-SH was used to selectively and sensitively monitor the ER thiol flux in the brains of mice with depression phenotypes.
Solution-processed wrinkled electrodes enable the development of stretchable electrochemical biosensors
Wearable biosensors are critical for enabling real-time and continuous health monitoring and disease management.
RNA chaperone assisted intramolecular annealing reaction towards oligouridylated RNA detection in cancer cells
An RNA chaperone assisted intramolecular annealing reaction for sensitive detection of genetic material in cell lysis and single cells was constructed.
Integrated microfluidic device for the separation, decomposition and detection of low molecular weight S-nitrosothiols
S-nitrosothiols (RSNOs) are electrokinetically separated and the nitrite generated by decomposition with mercury(II) is detected by amperometry, in an integrated microdevice.
Near-field infrared nanospectroscopy and super-resolution fluorescence microscopy enable complementary nanoscale analyses of lymphocyte nuclei
First near-field infrared spectroscopy and imaging of lymphocyte nucleus at 30 nm spatial resolution reveals spectrochemically distinct regions in nuclear organization.
How to unravel the chemical structure and component localization of individual drug-loaded polymeric nanoparticles by using tapping AFM-IR
We describe here the theoretical basis of the innovative tapping AFM-IR mode that can address novel challenges in imaging and chemical mapping of polymeric nanoparticles.
Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad
Fluxapyroxad high-affinity antibody generation and sensitive immunoassay development from a suitable hapten design.
A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability
The comparative study on linear vs. branched alkyl-chain amphiphiles indicates a favorable role for branched alkyl-chains in stabilizing membrane proteins.
Fourier transform infrared spectra of cells on glass coverslips. A further step in spectral pathology
FTIR spectra of cells on glass coverslips allows the study of the Amide I region.
A novel paper-based colorimetry device for the determination of the albumin to creatinine ratio
A novel paper-based analytical device (PAD) was fabricated and developed for the simple and rapid determination of the albumin to creatinine ratio (ACR) in urine samples.
Logical MS/MS scans: a new set of operations for tandem mass spectrometry
Described herein are novel sets of multiplexed MS/MS scans – termed ‘logical scans’ – performed on a linear ion trap.
Development of a selective and highly sensitive fluorescence assay for nucleoside triphosphate diphosphohydrolase1 (NTPDase1, CD39)
A new fluorescence-based assay is useful for monitoring CD39 reactions and enables low picomolar detection of nucleotides.
Interaction study of cancer cells and fibroblasts on a spatially confined oxygen gradient microfluidic chip to investigate the tumor microenvironment
This paper reports a single-layered microfluidic device for studying the interaction of cancer cells and fibroblasts in an oxygen gradient.
Biochemical alterations of Candida albicans during the phenotypic transition from yeast to hyphae captured by Fourier transform mid-infrared-attenuated reflectance spectroscopy
The first study that applies FTIR spectroscopy to monitor the biochemical modification of Candida albicans during the transition from a benign yeast to virulent hyphae using multiple spectral analyses.
Sensitive fluorescence detection of heparin based on self-assembly of mesoporous silica nanoparticle–gold nanoclusters with emission enhancement characteristics
Sensitive fluorescence detection of heparin based on self-assembly of mesoporous silica nanoparticle–gold nanoclusters with emission enhancement characteristics was reported.
A dual chemosensor for Cu2+ and Hg2+ based on a rhodamine-terminated water-soluble polymer in 100% aqueous solution
A dual chemosensor based on rhodamine-terminated PEG was developed for the simultaneous selective detection of Cu2+ and Hg2+ in water.
Detection of opium alkaloids in a Cypriot base-ring juglet
The first rigorous chemical evidence supporting the controversial theory linking Bronze Age Cypriot base-ring juglets with the opium poppy.
A background-subtraction strategy leads to ratiometric sensing of oxygen without recalibration
After background subtraction, oxygen sensors with different probe concentrations, instrumental setups, surface roughnesses, and supporting matrixes present identical calibration curves.
Digital enzyme assay using attoliter droplet array
Attoliter-volume and air-sealed reactor array for fast and robust single-molecule enzyme assay.
Highly sensitive detection of exosomes by SERS using gold nanostar@Raman reporter@nanoshell structures modified with a bivalent cholesterol-labeled DNA anchor
Exosomes, as important signal transmitters, play a key role in intercellular communication, especially in cancer metastasis.
Structuring polarity-inverted TBA to G-quadruplex for selective recognition of planarity of natural isoquinoline alkaloids
Selective small molecules’ planarity identification is first developed using the polarity inverted G-quadruplex as a high-performance selector.
2D europium coordination polymer as a regenerable fluorescence probe for efficiently detecting fipronil
A metal organic coordination polymer based fluorescent probe was used to detect fipronil for the first time. Luminescence intensities of 1 continuously reduced with increasing concentrations of fipronil, and still showed high fluorescence intensity after four cycles.
About this collection
This collection represents the top 10% of research published in Analyst. Featured articles are marked as HOT by the handling editor or as recommended by referees. Congratulations to all the authors whose articles are featured