Themed collection Analyst Recent HOT articles
Instrumentational implementation for parallelized nanopore electrochemical measurements
Nanopore electrochemistry, as one of the promising tools for single molecule sensing, has proved its capability in DNA sequencing and protein analysis.
Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges
Currently, there exists a multitude of different exosome isolation and characterization approaches based on biophysical and/or biochemical characteristics. Herein we review various technologies for separation of distinct exosome subpopulations.
Single enzyme electroanalysis
Traditional enzymology relies on the kinetics of millions of enzymes, an experimental approach that may wash out heterogeneities between individual enzymes. Electrochemical methods have emerged in the last 5 years to probe single enzyme reactivity.
Molecularly imprinted polymers for the detection of viruses: challenges and opportunities
Recent advancements regarding the application of MIPs and relevant virus imprinting techniques for the detection of viruses are highlighted.
Self-assembled nanomaterials for biosensing and therapeutics: recent advances and challenges
Self-assembled nanomaterials (SANs) exhibit designable biofunctions owing to their tunable nanostructures and modifiable surfaces.
DNA origami: an outstanding platform for functions in nanophotonics and cancer therapy
The utilization of the DNA origami technique in the fabrication of complex objects has given rise to possibilities in DNA-induced precise manufacturing and might open a new era of bottom-up fabrication.
An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19
Molecular biology and nanotechnology based analytical methods have proven to be promising diagnostic tools for the rapid diagnosis of COVID-19.
Gas-propelled biosensors for quantitative analysis
Gas-propelled biosensors display a simple gas-based signal amplification with quantitative detection features based on the target recognition event in combination with gas propulsion.
Recent advances in the use of stimulated Raman scattering in histopathology
Stimulated Raman histopathology with computer aided diagnosis.
Progress in electrochemiluminescence of nanoclusters: how to improve the quantum yield of nanoclusters
Classification of nanoclusters and methods to improve their quantum yield and applications.
Nucleic acid amplification-based microfluidic approaches for antimicrobial susceptibility testing
This review discusses the recent developments in microfluidics technology for antimicrobial susceptibility testing with a special emphasis on the nucleic acid amplification-based approach.
Approaches in metabolomics for regulatory toxicology applications
This tutorial review provides an overview of key concepts in metabolomics and their application to develop successful strategies in regulatory toxicology-oriented studies.
Applications of DNA-nanozyme-based sensors
In this review, the research progress of the sensors based on the DNA-nanozymes were summarized.
Smartphone-based optical analysis systems
The review describes the design, application and performance of current smartphone-based colorimeters, photo- and spectrometers and fluorimeters. Furthermore, it gives an overview of the advantages and disadvantages of such systems.
Rapid developments in lateral flow immunoassay for nucleic acid detection
Schematic of two main research directions of lateral flow assays for nucleic acid detection.
Multi-dimensional LC-MS: the next generation characterization of antibody-based therapeutics by unified online bottom-up, middle-up and intact approaches
This review presents an overview of current analytical trends in antibody characterization by multidimensional LC-MS approaches.
Graphene field-effect transistors as bioanalytical sensors: design, operation and performance
Changes in the electrical conductance of graphene field-effect transistors (GFETs) are used to perform quantitative analyses of biologically-relevant molecules such as DNA, proteins, ions and small molecules.
Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes
A simple target-responsive CRISPR/Cas12a-based strategy for translating molecular detection into a glucose test.
A ratiometric electrochemiluminescent cytosensor based on polyaniline hydrogel electrodes in spatially separated electrochemiluminescent systems
A three-channel ratiometric ECL platform for MCF-7 cells detection using potential-resolved ECL probes.
4-Mercaptobenzoic acid as a MALDI matrix for highly sensitive analysis of metals
4-Mercaptobenzoic acid was first introduced as a MALDI matrix for metal analysis. The developed method was successfully applied to the rapid screening and sensitive determination of metals in PM2.5 samples.
Acridinium benzoates for ratiometric fluorescence imaging of cellular viscosity
A series of fluorescent molecular rotors, acridinium benzoates, were designed for ratiometrically monitoring cellular viscosity with high sensitivity.
Photo-driven self-powered biosensors for ultrasensitive microRNA detection based on metal–organic framework-controlled release behavior
“Signal-on” photoelectrochemical biofuel cell-based self-powered biosensors based on metal–organic framework-controlled release behavior were constructed for ultrasensitive microRNA detection.
Ion-selective polymer dots for photoelectrochemical detection of potassium ions
Potassium-selective polymer dots (K-Pdots) containing potassium ionophores were for the first time used for photoelectrochemical (PEC) analysis and yielded sensitive and specific detection of potassium ions.
Quantifying cyanide in water and foodstuff using corrin-based CyanoKit technologies and a smartphone
This paper describes the detection of endogenous cyanide using corrin-based CyanoKit technologies in combination with a smartphone readout device.
Paper-based SERS analysis with smartphones as Raman spectral analyzers
We present a smartphone-based portable Raman spectrometer, which has a friendly human–machine interface, easy operation, rapid response time, and most importantly a very small size for on-site use.
Lectin inspired polymers based on the dipeptide Ser-Asp for glycopeptide enrichment
Lectin inspired polymers polySD-SiO2 were prepared and applied to the high-efficiency enrichment of glycopeptides.
Detection of Escherichia coli bacteria by impact electrochemistry
Employing the redox species N,N,N′,N′-tetramethyl-para-phenylene-diamine (TMPD), the label-free detection of E. coli, based on an electrochemical “on”-signal during impact electrochemistry, is reported for the first time.
A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering
We develop a ratiometric Raman probe for visualizing hydrogen sulfide in living cells as the first alkyne-based sensor for SRS microscopy.
In vivo detection of drug-induced apoptosis in tumors using Raman spectroscopy
We describe a label-free approach based on Raman spectroscopy, to study drug-induced apoptosis in vivo.
Highly sensitive and on-site NO2 SERS sensors operated under ambient conditions
This paper presents the first demonstration of highly sensitive and on-site SERS-based NO2 sensors with a detection limit of 0.1 ppm operated under ambient conditions.
A new enzyme immunoassay for alpha-fetoprotein in a separate setup coupling an aluminium/Prussian blue-based self-powered electrochromic display with a digital multimeter readout
A new immunoassay was designed for the detection of disease-related biomarkers (alpha-fetoprotein, AFP, as a model), coupling an aluminium (Al)/Prussian blue-based electrochromic display with a digital multimeter readout.
Wettability alteration in a functional capillary tube for visual quantitative point of care testing
A quantified POCT device was designed based on wettability alteration in a capillary tube.
A novel electrochemiluminescence biosensor based on S-doped yttrium oxide ultrathin nanosheets for the detection of anti-Dig antibodies
The mechanism of a novel electrochemiluminescence biosensor based on S-doped yttrium oxide ultrathin nanosheets for detection of anti-Dig antibodies.
Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction
We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Small molecule–protein interactions in branch migration thermodynamics: modelling and application in the homogeneous detection of proteins and small molecules
A homogeneous assay for proteins and small molecules with ultra-high inhibition factors and sensitivity was established.
A thumb-size electrochemical system for portable sensors
A thumb-size and precise electrochemical system is designed for sensors in size and cost sensitive applications.
An ultrasensitive photoelectrochemical bioanalysis strategy for tumor markers based on the significantly enhanced signal of a bismuth oxyiodine microsphere/graphitic carbon nitride composite
An ultrasensitive PEC bioanalysis strategy was designed based on the significantly enhanced signal of BiOI/g-C3N4 and amplified signal variation of CuS NPs.
Time-resolved visual detection of heparin by accelerated etching of gold nanorods
Plasmonic gold nanorods are promising and sensitive light scattering probes, which can reach the single particle level. Herein, we present the scattering properties of gold nanorods for time-resolved visual detection of heparin based on the rapid etching of gold nanorods under dark-field microscopy.
Deducing disulfide patterns of cysteine-rich proteins using signature fragments produced by top-down mass spectrometry
With improved deconvolution and fragment identification strategies, we use the patterns of specific modifications of fragment ions resulted from disulfide cleavage in top-down mass spectrometry to deduce the complex disulfide patterns of cysteine-rich proteins.
Electrokinetic elucidation of the interactions between persistent luminescent nanoprobes and the binary apolipoprotein-E/albumin protein system
The interactions between NPs and a binary system of proteins were studied through a systematic set of capillary electrophoresis models. The results revealed precise details of the soft and hard protein corona formation and dynamics.
Evolution of DNA aptamers against esophageal squamous cell carcinoma using cell-SELEX
Several aptamers specific to ESCC cells were successfully screened using cell-SELEX, and a truncated ultrashort aptamer was found suitable for imaging ESCC tissues of patients.
Structural analysis of petroporphyrins from asphaltene by trapped ion mobility coupled with Fourier transform ion cyclotron resonance mass spectrometry
Combination of experimentally determined collision-cross sections provided by trapped ion mobility high-resolution mass spectrometry with in-silico calculations for structural assessment of petroporphyrins within an ultra-complex crude oil matrix.
Sensitive electrochemical immunosensor using a bienzymatic system consisting of β-galactosidase and glucose dehydrogenase
A sensitive electrochemical immunosensor based on a bienzymatic system consisting of β-galactosidase and flavin adenine dinucleotide-dependent glucose dehydrogenase was developed.
An automatic flow-through system for exploration of the human bioaccessibility of endocrine disrupting compounds from microplastics
Oral bioaccessibility of plastic migrants using automatic flow methodology.
Microplasma-induced vapor generation for rapid, sample preparation-free screening of mercury in fruits and vegetables
A simple, sensitive method based on microplasma-induced chemical vapor generation for the rapid screening and quantification of mercury in fruits and vegetables by atomic spectrometry without sample preparation.
A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils
A broad-specific anti-B[a]P mAb (4E8) was prepared with IC50 value of 2.51 ng mL−1. The GNP-ICS assay showed broad specificity in the detection of B[a]P and its analogues, with cut-off and vLOD values of 100 and 10 ng mL−1, respectively.
TiN nanopillar-enhanced laser desorption and ionization of various analytes
TiN nanopillar was applied as a robust analytical substrate for the laser desorption/ionization mass spectrometry of various types of analytes with a wide mass range. Numerical simulation validated the heat confinement effect of the pillar shape.
Hyper-fast gas chromatography and single-photon ionisation time-of-flight mass spectrometry with integrated electrical modulator-based sampling for headspace and online VOC analyses
We developed a novel fast gas chromatography (fastGC) instrument with integrated sampling of volatile organic compounds (VOCs) and detection by single-photon ionisation (SPI) time-of-flight mass spectrometry (TOFMS).
Thin film molecularly imprinted polymer (TF-MIP), a selective and single-use extraction device for high-throughput analysis of biological samples
A thin film molecularly imprinted polymer (TF-MIP) is developed for isolation of tricyclic antidepressants (TCAs). This single use microextraction device is applied for selective and high-throughput extraction of TCAs from biological matrices.
Rapid, in situ detection of chemical warfare agent simulants and hydrolysis products in bulk soils by low-cost 3D-printed cone spray ionization mass spectrometry
A new ambient ionization method, 3D-printed cone spray ionization mass spectrometry (3D-PCSI-MS), is implemented for rapid profiling of chemical warfare agent (CWA) simulants in unprepared soil samples.
HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification
An HIV detection assay featuring a low-cost μPAD for electrophoretic RNA extraction and rapid isothermal nucleic acid amplification.
A carbon dot doped lanthanide coordination polymer nanocomposite as the ratiometric fluorescent probe for the sensitive detection of alkaline phosphatase activity
Based on the stimulus-responsive lanthanide coordination polymer and the stable fluorescence of carbon dots (CDs), a simple ratiometric fluorescence sensing strategy was proposed to detect ALP activity.
Spectral characterisation of a traditional Mesoamerican dye: relationship between in situ identification on the 16th century Codex Borbonicus manuscript and composition of Justicia spicigera plant extract
The identification of Justicia spicigera in the Codex Borbonicus by Raman spectroscopy motivated the development of a multi-analytical techniques approach to broaden the physico-chemical knowledge regarding this traditional dye source.
A modular microscopic smartphone attachment for imaging and quantification of multiple fluorescent probes using machine learning
A smartphone-based fluorescent microscope capable of imaging multiple fluorescent probes at different magnification levels.
A dual-channel incoherent broadband cavity-enhanced absorption spectrometer for sensitive atmospheric NOx measurements
A novel dual cavity UV-VIS spectrometer for sensitive monitoring of urban NOx and other interfering pollutants.
Comparative evaluation and design of a G-triplex/thioflavin T-based molecular beacon
Compared with the common G-quadruplex-based molecular beacon, the G-triplex-based molecular beacon exhibits higher sensitivity and faster response speed.
Tryptamine-functionalized magnetic nanoparticles for highly sensitive detection of Salmonella typhimurium
A workflow for bacterial pathogen enrichment and DNA extraction and isolation in the single-tube sample preparation method for nucleic acid-based detection of nontyphoidal salmonella is presented.
Supramolecular self-assembly and perfected in vitro/vivo property of 5-fluorouracil and ferulic acid on the strength of double optimized strategy: the first 5-fluorouracial-phenolic acid nutraceutical cocrystal with synergistic antitumor efficacy
The first 5-fluorouracil-nutraceutical cocrystal with synergistic antitumor effect, Flu-FR-H2O, is synthesized based on the dual optimization strategy and evaluated by combining the experimental evaluation with theoretical analysis.
Location of carbon–carbon double bonds in unsaturated lipids using microdroplet mass spectrometry
Microdroplet mass spectrometry for the location of carbon–carbon double bonds in unsaturated fatty acids and lipids via ROS generation at the air–water interface.
Predicting the conformations of the silk protein through deep learning
Deep learning models are developed to identify the silk proteins and evaluate their relative content of each conformation from FTIR spectra. These models are highly accurate and time-efficient and promise to process massive FTIR data sets.
Simultaneously monitoring endogenous MAPK members in single living cells by multi-channel fluorescence correlation spectroscopy
A new strategy for in situ and simultaneously monitoring endogenous MAPK kinases in single living cells based on a multi-channel FCS system and affinity fluorescent probes is developed.
Dissociation of large gaseous serine clusters produces abundant protonated serine octamer
Dissociation of large multiply protonated serine clusters produces abundant protonated octamer at an optimum ion temperature predominantly through a doubly protonated decamer intermediate.
Catalysis-induced performance enhancement of an electrochemical microcystin-LR aptasensor based on cobalt-based oxide on a B, N co-doped graphene hydrogel
An electrochemical sensor based on a 3D cobalt-based oxide modified boron and nitrogen co-doped graphene hydrogel exhibited excellent sensitivity and catalytic induction properties.
Droplet-based blood group antibody screening with laser incubation
Complete indirect antiglobulin test (IAT) in a single diagnostic incorporating a laser-incubated droplet on a hydrophobic surface with read-out provided by a paper strip.
DNA nanolantern-based split aptamer probes for in situ ATP imaging in living cells and lighting up mitochondria
We designed DNA nanolantern-based split aptamer nanoprobes for in situ ratiometric analysis of ATP expression in living cells.
A metal–organic framework with multienzyme activity as a biosensing platform for real-time electrochemical detection of nitric oxide and hydrogen peroxide
A metal–organic framework with multienzyme activity was designed as an electrochemical material for real-time electrochemical detection of nitric oxide and hydrogen peroxide.
A combined flow injection/reversed-phase chromatography–high-resolution mass spectrometry workflow for accurate absolute lipid quantification with 13C internal standards
A fully automated novel workflow for lipidomics based on flow injection, followed by reversed phase liquid chromatography–high-resolution mass spectrometry in combination with LILY as internal standard, enabling accurate quantification via RP-LC.
Development of a piperazinyl-NBD-based fluorescent probe and its dual-channel detection for hydrogen sulfide
To selectively detect H2S, a novel dual-channel fluorescence probe, triphenylamine piperazine NBD (TPA-Pz-NBD), was developed based on the thiolysis reaction of NBD amine.
Fabrication of an electrochemical biosensor composed of multi-functional Ag ion intercalated DNA four-way junctions/rhodium nanoplate heterolayer on a micro-gap for C-reactive protein detection in human serum
As inflammation plays a role in the pathogenesis of acute coronary syndromes, C-reactive protein (CRP) can be used as a biomarker. The present study constructed the electrochemcial biosensor with nanobio hybrid material for CRP detection.
In vitro measurement of superoxide dismutase-like nanozyme activity: a comparative study
Analyzing the SOD-like activity of nanozymes in vitro is of great importance for identifying new nanozymes and predicting their potential biological effects in vivo.
About this collection
This collection represents the top 10% of research published in Analyst. Featured articles are marked as HOT by the handling editor or as recommended by referees. Congratulations to all the authors whose articles are featured