A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

chen zeng; Fanghao Hu; Rong Long; Wei Min

doi:10.1039/C8AN00910D

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

chen zeng, Fanghao Hu, Rong Long and Wei Min

Abstract

Stimulated Raman scattering (SRS) coupled with alkyne tags has been an emerging imaging technique to visualize small-molecule species with high sensitivity and specificity. Here we describe the development of a ratiometric Raman probe for visualizing hydrogen sulfide (H2S) species in living cells, as the first alkynebased sensor for SRS microscopy. This probe uses an azide unit as a selective reactive site, and it targets mitochondria with high specificity. The SRS ratiometric images display strong response to H2S level changes in living cells.

Supplementary files

Publication details

The article was received on 16 May 2018, accepted on 31 Aug 2018 and first published on 03 Sep 2018

Article type: Communication

DOI: 10.1039/C8AN00910D

Citation: Analyst, 2018, Accepted Manuscript

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A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

C. zeng, F. Hu, R. Long and W. Min, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00910D
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Analyst

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

Abstract

Supplementary files

Publication details

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

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