A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

Chen Zeng; Fanghao Hu; Rong Long; Wei Min

doi:10.1039/C8AN00910D

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

Chen Zeng,^a Fanghao Hu,^a Rong Long^a and Wei Min*^a

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* Corresponding authors

^a Department of Chemistry, Columbia University, 3000 Broadway, New York, USA
E-mail: wm2256@columbia.edu

Abstract

Stimulated Raman Scattering (SRS) coupled with alkyne tags has been an emerging imaging technique to visualize small-molecule species with high sensitivity and specificity. Here we describe the development of a ratiometric Raman probe for visualizing hydrogen sulfide (H₂S) species in living cells as the first alkyne-based sensor for SRS microscopy. This probe uses an azide unit as a selective reactive site, and it targets mitochondria with high specificity. The SRS ratiometric images show a strong response to H₂S level changes in living cells.

This article is part of the themed collections: Recent HOT articles and Analyst Emerging Investigator Lectureship winners

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Publication details

The article was received on 16 May 2018, accepted on 31 Aug 2018 and first published on 03 Sep 2018

Article type: Communication

DOI: 10.1039/C8AN00910D

Citation: Analyst, 2018,143, 4844-4848

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A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

C. Zeng, F. Hu, R. Long and W. Min, Analyst, 2018, 143, 4844
DOI: 10.1039/C8AN00910D
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A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

Abstract

Supplementary files

Publication details

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

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