SPEC 2018: International Society of Clinical Spectroscopy
Welcome to the latest themed issue on the topic of Optical Diagnosis in Analyst, guest-edited by Dr. Matthew Baker.
Prospective on using fibre mid-infrared supercontinuum laser sources for in vivo spectral discrimination of disease
Mid-infrared (MIR) fibre-optics may play a future role in in vivo diagnosis of disease, including cancer.
Biomedical applications of mid-infrared quantum cascade lasers – a review
Quantum cascade lasers with high spectral power density and narrow band emission profiles provide novel access to biomedical applications.
Clinical applications of infrared and Raman spectroscopy: state of play and future challenges
This review examines the state-of-the-art of clinical applications of infrared absorption and Raman spectroscopy, outstanding challenges, and progress towards translation.
Dual purpose fibre – SERS pH sensing and bacterial analysis
A way to incorporate SERS nanosensors on the end of an optical fibre that also allows for the extraction of bacterial samples.
Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell
First IR-SNOM images over the Amide I band taken in the centre of an oesophageal cancer cell.
Three-dimensional depth profiling of prostate tissue by micro ATR-FTIR spectroscopic imaging with variable angles of incidence
Variable angle micro ATR-FTIR, via the insertion of circular apertures, was used to measure tissue samples at various penetration depths.
Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480
A novel photosensitiser, DC473, designed with solvatochromatic fluorescence and distinct Raman signal, is detected with tandem fluoRaman in SW480 cells.
Characterization of inflammatory breast cancer: a vibrational microspectroscopy and imaging approach at the cellular and tissue level
Inflammatory breast cancer (IBC) has a poor prognosis because of the lack of specific biomarkers and its late diagnosis.
Hyperspectral analysis applied to micro-Brillouin maps of amyloid-beta plaques in Alzheimer's disease brains
Non-negative factorization analysis applied to spontaneous Brillouin microscopy maps of amyloid-beta plaques in a transgenic mouse model enables to resolve spatially distinct components with specific mechanical properties.
Optimised spectral pre-processing for discrimination of biofluids via ATR-FTIR spectroscopy
Pre-processing is an essential step in the analysis of spectral data.
Exploring the efficacy and cellular uptake of sorafenib in colon cancer cells by Raman micro-spectroscopy
This study demonstrates the efficacy and distribution of sorafenib in colon cancer cells by label-free Raman microscopy.
Different binding sites of serum albumins in the protein corona of gold nanoparticles
Binding sites of albumins on gold nanoparticles were characterized by surface-enhanced Raman scattering.
Raman spectroscopic features of primary cardiac microvascular endothelial cells (CMECs) isolated from the murine heart
Gaining knowledge on the biochemical profile of primary endothelial cells on a subcellular level can contribute to better understanding of cardiovascular disease.
ATR-FTIR spectroscopy shows changes in ovarian cancer cells after incubation with novel organoamidoplatinum(II) complexes
ATR-FTIR spectroscopy has been applied to compare the effect of new organoamidoplatinum(II) complexes with cisplatin on cells from a cisplatin-sensitive and a cisplatin-resistant ovarian cancer cell line.
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
Translating Raman spectroscopy for colorectal cancer diagnosis with a focus on high-throughput design, inter-user variability and sample handling considerations.
Dual excitation wavelength system for combined fingerprint and high wavenumber Raman spectroscopy
A probe-based Raman spectroscopy system using a single laser module with two excitation wavelengths has been developed for measuring the fingerprint and high wavenumber regions with a single detector.
New insights of Raman spectroscopy for oral clinical applications
Oral injuries are currently diagnosed by histopathological analysis of biopsy, which is an invasive procedure and does not give immediate results.
Raman spectroscopy of stored red blood cell concentrate within sealed transfusion blood bags
Spectral information relevant to the quality of stored blood can be obtained in situ through sealed blood transfusion bags using a commercially available instrument.
Towards a quantum cascade laser-based implant for the continuous monitoring of glucose
An in vitro investigation of a quantum cascade laser-based transflection optofluidic interface for the continuous glucose monitoring in biofluids is presented with respect to its glucose sensitivity and specificity, its glucose exchange time, its resistance against temperature variations and its long-term stability.
Optimizing decision tree structures for spectral histopathology (SHP)
This paper reviews methods to arrive at optimum decision tree or label tree structures to analyze large SHP datasets.
Near-field infrared nanospectroscopy and super-resolution fluorescence microscopy enable complementary nanoscale analyses of lymphocyte nuclei
First near-field infrared spectroscopy and imaging of lymphocyte nucleus at 30 nm spatial resolution reveals spectrochemically distinct regions in nuclear organization.
Raman spectroscopic analysis of high molecular weight proteins in solution – considerations for sample analysis and data pre-processing
This study explores the potential of Raman spectroscopy, coupled with multivariate regression techniques and ion exchange chromatography, to quantitatively monitor diagnostically relevant changes in high molecular weight proteins in liquid plasma.
Raman spectroscopy as a novel tool for fast characterization of the chemical composition of perivascular adipose tissue
One of the new targets of untapped therapeutic potential is perivascular adipose tissue (pVAT). Based on Raman spectroscopy we demonstrated that the lipid unsaturation degree was clearly distinct in various types of adipose tissues, in particular thoracic and abdominal pVATs, and was influenced by the age of animals.
How to unravel the chemical structure and component localization of individual drug-loaded polymeric nanoparticles by using tapping AFM-IR
We describe here the theoretical basis of the innovative tapping AFM-IR mode that can address novel challenges in imaging and chemical mapping of polymeric nanoparticles.
Diagnosis of pancreatic cancer via1H NMR metabolomics of human plasma
The investigation of blood plasma via1H NMR metabolomics revealed a panel of potential biomarkers of pancreatic cancer.
Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model
PCA score of FTIR spectra in fingerprint region for cultured chondrocytes at day 7, 14 and 21 in HA hydrogel showing that the 3D culture drove chondrocytes (CY) to chondron-like (CN) structure dynamically.
Surface enhanced resonance Raman spectroscopy (SERRS) for probing through plastic and tissue barriers using a handheld spectrometer
Through tissue imaging of a live breast cancer tumour model using handheld surface enhanced resonance Raman spectroscopy (SERRS).
Blood-based near-infrared spectroscopy for the rapid low-cost detection of Alzheimer's disease
Plasma samples deposited on low-E slides were analysed in transmission mode by using a FT-NIR spectrometer in order to detect Alzheimer's disease using computer-based methods.
Non-invasive analysis of stored red blood cells using diffuse resonance Raman spectroscopy
A method to acquire the Raman spectra of sub-surface components using diffusely focused radiation in a microscope sampling configuration is described.
About this collection
This collection of articles highlights work from the 10th conference in the successful SPEC series, held in Glasgow, UK, 10 June – 15 June 2018. The meeting featured research from scientists and clinicians, both from academia and industry, who are developing spectroscopic instrumentation and techniques to improve world health. Guest Edited by Conference Organising Committee Chair and Analyst Advisory Board Member Matthew Baker (University of Strathclyde, UK), the collection includes a topical review article by Hugh J. Byrne et al. titled “ Clinical applications of infrared and Raman spectroscopy: state of play and future challenges”.