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How to Unravel the Chemical Structure and Component Localization of Individual Drug-Loaded Polymeric Nanoparticles by Using Tapping AFM-IR

Abstract

AFM-IR is a photothermal technique which combines AFM and infrared (IR) spectroscopy to unambiguously identify the chemical composition of a sample with tens of nanometer spatial resolution. So far, it was successfully used in contact mode in a variety of applications. However, contact mode is unsuitable for soft or loosely-adhesive samples such as polymeric nanoparticles (NPs) of less than 200 nm of wide interest for biomedical applications. We describe here the theoretical basis of the innovative tapping-AFMIR mode that can address novel challenges in imaging and chemical mapping. The new method enables gaining information not only on NP morphology and composition, but also revealed drug location and core-shell structures. If up to now the locations of NP components could only be hypothesized, tapping AFM-IR allows to accurately visualizing both the location of the NPs’ shells and of the incorporated drug, pipemidic acid. The preferential accumulation of the drug in the NPs’ top layers is proved, despite its low concentration (<1 wt%). These studies pave the way towards the use of tapping AFM-IR as a powerful tool to control the quality of NP formulations based on individual NP detection and component quantification.

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Publication details

The article was received on 06 Jul 2018, accepted on 23 Sep 2018 and first published on 25 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01239C
Citation: Analyst, 2018, Accepted Manuscript
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    How to Unravel the Chemical Structure and Component Localization of Individual Drug-Loaded Polymeric Nanoparticles by Using Tapping AFM-IR

    J. Mathurin, E. Pancani, A. Deniset, K. Kjoller, C. Prater, R. Gref and A. dazzi, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01239C

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