Themed collection In memory of Craig Lunte

Recent innovations in microscale western blotting are surveyed, and the potential for enhancing detection using advances in label-free biosensing is briefly discussed.

A mini-review that focuses on the most recent trends in 3D-printed microfluidic devices, with sample designs that can be downloaded for use.

Sarcosine is an amino acid that has been listed as a new indicator for prostate cancer.

We describe the development of a paper electrophoresis chip integrated with pencil electrodes for contactless conductivity detection and its application in the separation of biomolecules associated with kidney dysfunctions.

Utilization of polyimide resin and 3D printed mold provides a novel, rapid, and low cost alternative for carbon-fiber microelectrode fabrication.

In this work, an electrochromic sensing platform with prussian blue (PB) as the indicator was proposed for signaling carcinoembryonic antigen (CEA) using the bipolar electrode (BPE) system.

The electrochemical behaviour of fucoidan, a sulfated polysaccharide, was investigated, leading to a detection strategy by adsorptive stripping voltammetry.

Microfluidic discs integrating silica-C18 and carbon monolithic columns were developed for sample preparation.

Fluorescence derivatization allows for detection of lipid peroxidation biomarkers of epilepsy-induced oxidative stress.

Phenolic acids in Irish whiskeys were determined using CE-UV with field amplified sample stacking. Length of aging and prior use of cask influenced phenolic acid profiles.

A microfluidic platform was used to investigate the antioxidant properties of carnosine, an endogenous dipeptide.

In this paper, we use DEP with insulating structures (iDEP) to generate a non-uniform electric field for trapping gold nanoparticles (AuNP). The system was coupled to a Raman spectrometer for the detection of Crystal Violet by utilizing the SERS effect.

Insulin secretion from islets of Langerhans is a dynamic process that is essential for maintaining glucose homeostasis.

Checkpoints in processing of metabolomics data are essential to reliably define preliminary identifications of metabolomic studies.

The application of a single-walled carbon nanohorn for a molecularly imprinted sensor has been demonstrated for the first time.

Simplified sample collection methods can continue to advance the field of biosensors by decreasing the cost required to acquire and store samples and/or increasing the number of locations where the sensors can be used.

The ethylene/norbornene content within cyclic olefin copolymer (COC) is well known to affect the chemical and physical properties of the copolymer, such as the glass transition temperature (T_g) and transparency.

Speciation controls the chemical behavior of trace metals.

A novel fibre-based microfluidic methodology was developed to move and isolate charged solutes, biomolecules, and intact bacterial cells, based upon a novel multi-functional 3D printed platform.

The study of transmitter interactions in the brain requires methodology to detect stimulus-driven neurotransmitter release. This report introduces an enzyme-coated 7 μm carbon-fiber microelectrode used with fast-scan cyclic voltammetry to detect evoked acetylcholine release in mouse brain slices.

A glassy carbon electrode was modified by electrodeposition of a thin layer of poly(caffeic acid) and optimized for highly selective and sensitive detection of L-DOPA.

On-chip preconcentration, purification, and fluorescent labeling are desirable sample preparation steps to achieve complete automation in integrated microfluidic systems.

The absorption spectra of single-component mixtures of six lanthanide chloride salts were obtained in the molten salt eutectic 3LiCl–2CsCl at 723 K, and used to build multivariate regression models to predict concentrations of multi-component mixtures of these metals in solution.

In this study, a microfluidic plasma-separation device that realizes the whole blood analysis of C-reactive protein (CRP) using one drop of blood is developed.

A disposable paper microfluidic device was developed to analyse different S-nitrosothiols simultaneously decomposed by Hg²⁺ as well as UV, Vis and IR lamps.

A novel on-line microfluidic assay for the chemotherapy agent carboplatin will allow sensitive detection of the drug directly applied to the tumour as it emerges from the tumour into healthy tissue.

This work describes for the first time the coupling of microfluidic chips (MC) to electrosynthetized silver metallic oxide clusters (AgMOCs).

We report on the analytical performance of a tetrahedral amorphous carbon (ta-C:N) thin-film electrode in flow injection analysis with amperometric detection.

Battery operation of a closed-BPE dual cell with colorimetric readout by smartphone camera yields a simple, inexpensive, field-deployable electrochemical sensor.

A peptidase-resistant ABL kinase substrate was developed by identifying protease-susceptible bonds on an ABL substrate peptide and replacing flanking amino acids with non-native amino acids.

A novel in-gel bioanalytical immunodetection method has been developed to determine both the presence and the location of cocaine on the surface of banknotes.

FSCV can be utilized to predict solution formation constants.

We demonstrate the capabilities of a centrifugal polyethylene terephthalate toner (PeT) microdevice for genetic analysis of short tandem repeats (STR) via PCR amplification.

The use of overhead transparencies as substrates for the fabrication of microfluidic devices brings new capabilities in the creation of fully-integrated systems.

Glyconanoparticles have been synthesised for the specific detection of mSiglec-E in solution and expressed on the surface of CHO cells.

A microfluidic electrochemical sensor array detects DNA oxidation and adduct formation for a fast genotoxicity chemistry screen for test compounds.

A combination of 3D printed tools and classic cellular analyses reveals the benefits of reducing the glucose content of currently approved solutions used to process and store red blood cells for transfusion medicine.

Scanning electrochemical microscopy (SECM) in the constant-height mode was used to image individual unbiased gold nanowires (AuNWs, 2–3 μm long and ~140 nm diameter) covalently linked with horseradish peroxidase (HRP).

Dengue diagnosis was performed on printed microzones based on colorimetric detection of IgG and IgM biomarkers in serum samples through enzyme-linked-immunosorbent assay experiments.

An air focusing technique was used to directly electrospin fibers into fully sealed microfluidic devices for 3D cell culture.

The use of monoclonal antibodies (mAbs) for the manufacture of innovator and biosimilar biotherapeutics has increased tremendously in recent years.

Simultaneous patch clamp and fast-scan cyclic voltammetry measurements were performed to monitor cell behavior during iontophoretic drug delivery.

This manuscript reports a novel and facile method for PQ-10/SLS separation and subsequent PQ-10 quantification via potentiometric titration.

Quantification of benfotiamine and sulbutiamine, synthetic thiamine analogs, in biological samples is an essential step toward understanding the role of these thiamine analogs on cancer cell proliferation.

Non-toxic, fluorescent carbon nanodot labels are employed as novel ratiometric immunosensors for α-fetoprotein (AFP), a liver cancer biomarker. The assay generates a broad linear range, a low detection limit, and can be adapted to a variety of immunoassay targets.

A reliable, label-free, ion suppression-free Sirtuin 1 assay has been developed. By interfacing multi-well plates to electrospray ionization mass spectrometry by oil-segmented droplets, the assay can be applied for high throughput Sirtuin 1 modulator screening.

A sheath liquid capillary electrophoresis-mass spectrometry interface was used to develop a qualitative method for analysis of 14 flavones, flavonols, flavonones, and representative glycosides in plant samples.

A metal-free silk carbon–ionic liquid composite, synthesized from natural silk cocoons, was prepared for electrochemical determination of dopamine.

An electrochemical sensor for measuring total cholesterol in serum using Triton X-100 as a printed, non-enzymatic electrocatalyst for hydrogen peroxide and for the solubilisation of lipoproteins.

This study demonstrates the application of an OECS as an in vivo method to investigate the dynamic change of ascorbate in the olfactory bulb of rats during the acute period of olfactory dysfunction.

We report development of a mobile and easy-to-fabricate theta pipette microfluidic device for segmented flow sampling.