Themed collection Most popular 2023 chemical biology articles

Targeted protein degradation strategies employing proteins as binders for degradation targets.

In this review, we present the key synthetic strategies for the preparation of various ubiquitinated proteins and the application of these conjugates in biochemical and functional studies.

Biological species, including reactive oxygen species (ROS), reactive sulfur species (RSS), reactive nitrogen species (RNS), F⁻, Pd²⁺, Cu²⁺, Hg²⁺, and others, are crucial for the healthy functioning of cells in living organisms.

A primer that covers the discovery and validation process for novel aptamers and highlights recent applications of aptamers in biosensing and cell separations.

This work reviewed the new progress of conductive hydrogels in the application of tissue repair and provided a useful reference for further preparation of safer and more efficient conductive hydrogels for tissue engineering.

This review presents discrete metal complexes that catalyse or photocatalyse reactions within living cells or living organisms.

This review provides the recent progress in the application of inorganic-based biomaterials for rapid hemostasis and wound healing, including preparation methods, material systems, intrinsic mechanisms and biofunctions.

A melamine-based glucoside, MG-C11, has the ability to form a dynamic hydrogen-bonding network between detergent molecules, responsible for the markedly enhanced efficacy for GPCR stabilization compared to LMNG and previously developed TTG-C11.

Small molecule degraders and stabilizers of squalene synthase based on the same scaffold are reported, which respectively accelerate or block its natural degradation.

In this work, we describe the engineering of a microbial platform to produce six different C₄ chemicals at near quantitative yields using a combination of metabolic engineering and directed evolution.

Sulfolactate is an important species in the biogeochemical sulfur cycle. Herein we report the 3D cryo-EM structure and kinetics of its biosynthetic enzyme, sulfolactaldehyde dehydrogenase.

A Ru-based artificial metalloenzyme (ArM) at just 1–5 mol% could catalyze olefin metathesis in blood to construct various molecular scaffolds. The cancer-targeting ArM at a low dosage could elicit tumor growth inhibition by in vivo drug synthesis.

Tannic acid (TA) mitigates ferroptosis induced by amyloid β-Fe in Alzheimer's disease (AD) through a multipronged activity. TA activates and enhances the GPX4 levels to counteract the complex interaction between AD and ferroptosis.

Multiscale simulations in combination with time-dependent density functional theory are used to explore the energetics, localization, and spectroscopic properties of triplet states in the reaction center of Photosystem II.

We developed ²¹¹At-radiolabeled 2,6-diisopropylphenyl azide (ADIPA) for targeted α-particle therapy. In the experiment using a mouse model, low-dose (70 kBq) administration of ADIPA effectively suppressed tumor growth without causing adverse effects.

DNA-lipid tethers can replace ACE2 receptor attachment for SARS-CoV-2 entry. An attachment factor and protease are sufficient for membrane fusion, but ACE2 added in trans speeds up fusion kinetics.

A series of peptidomimetic Sortase A inhibitors is reported. These compounds show inhibition activity of the Sortase A enzyme and efficiently prevent biofilm formation of S. aureus.

The molecular mechanisms underlying molecular glue protein complex stabilization are poorly understood. In this study, we kinetically investigate covalent molecular glue stabilization of a canonical protein–protein interaction using native mass spectrometry.

Serum albumin–Co²⁺ interactions are of clinical importance.

A photo-caged STING agonist featuring a tumor-targeting carbonic anhydrase warhead was designed and synthesized for photo-controllable activation of STING signaling.

Fluorescent probes with a designed unnatural warhead for binding to PYP-tag enabled intracellular/cell-surface selective protein labeling. This unique imaging tool was successfully applied to reveal multiple subcellular localizations of GLUT4.

Metabolic labelling of a specific post-translational modification in cells with a bifunctional probe enables site-specific photocrosslinking, enrichment and identification of PTM-mediated protein–protein interactions through quantitative proteomics.

An iridium(III) two-photon photosensitizer was demonstrated to effectively trigger melanoma cell death by a combination of ferroptosis and ICD, and also cause the systemic induction of an immune response.

Here, we report an Ir(III) complex that contains a novel chiral pyridine RAS-selective lethal ligand and effectively inhibits GPX4 and FSP1 to induce ferroptosis in human fibrosarcoma (HT-1080) cells.