Liquid biopsy: a perspective for probing blood for cancer
Thought leaders Stefanie Jeffrey and Mehmet Toner introduce the Lab on a Chip Personalised Medicine: Liquid Biopsy thematic collection.
Microfluidic engineering of exosomes: editing cellular messages for precision therapeutics
This review examines scientific advancements of microfluidic technology for engineering exosomes and assesses future applications and perspectives in developing precision therapeutics, which can serve the community by identifying potential new research areas or technologies that are urgently needed in precision therapeutics.
Extracellular vesicles as cancer liquid biopsies: from discovery, validation, to clinical application
Substantial research has been devoted to elucidate the roles that extracellular vesicles (EVs) play in the regulation of both normal and pathological processes, and multiple studies have demonstrated their potential as a source of cancer biomarkers.
Cancer diagnosis: from tumor to liquid biopsy and beyond
Research on circulating biomarkers have enabled the development of new diagnostic tools that complement conventional biopsies through less invasive sampling of the tumor cells, also known as liquid biopsy.
Machine learning to detect signatures of disease in liquid biopsies – a user's guide
New technologies that measure sparse molecular biomarkers from easily accessible bodily fluids (e.g. blood, urine, and saliva) are revolutionizing disease diagnostics and precision medicine.
Circulating tumor DNA and liquid biopsy: opportunities, challenges, and recent advances in detection technologies
Opportunities and challenges in translational application of ctDNA along with recent developments in chip-based ctDNA detection technologies have been reviewed.
Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine
Breakthroughs in adapting microfluidic systems for exosome isolation, detection, and analysis are providing new tools to revolutionize personalized medicine.
Novel nanosensing technologies for exosome detection and profiling
This article reviews recent advances of new assay platforms that are developed to facilitate molecular analyses of exosomes.
Microfluidics-enabled rational design of immunomagnetic nanomaterials and their shape effect on liquid biopsy
A microfluidics-enabled strategy for the controllable synthesis of immunomagnetic nanomaterials was developed, and the shape-dependent screening efficiency of CTCs was investigated.
Microfluidic concentration and separation of circulating tumor cell clusters from large blood volumes
Rare CTC clusters can be purified intact from large blood volumes with a continuous three-stage non-equilibrium inertial separation array (NISA).
Hybrid negative enrichment of circulating tumor cells from whole blood in a 3D-printed monolithic device
A monolithic 3D-printed microfluidic device integrated with stacked layers of functionalized leukodepletion channels and microfiltration for the negative enrichment of circulating tumor cells directly from clinically relevant volumes of whole blood.
Device for whole genome sequencing single circulating tumor cells from whole blood
To sequence single circulating tumor cells (CTCs) from whole blood, a microfluidic chip was developed to perform blood filtering/CTC enrichment/CTC sorting and in situ MDA for whole genome sequencing.
Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells
Integrated ferrohydrodynamic cell separation (iFCS) explores cell magnetization in biocompatible ferrofluids and enriches CTCs in an antigen-independent and cell size variation-inclusive manner.
Tumor cell capture from blood by flowing across antibody-coated surfaces
Here we presented a set-up which can be used to capture cancer cells in continues flow fusing a functionalized surface.
An ultrasensitive test for profiling circulating tumor DNA using integrated comprehensive droplet digital detection
We present an ultra-sensitive, novel liquid biopsy approach which can uniquely enable detection of CTCs using genetic markers without pre-enrichment.
Cancer marker-free enrichment and direct mutation detection in rare cancer cells by combining multi-property isolation and microfluidic concentration
We present a novel cancer marker-free CTC enrichment method by size-based filtration and immunomagnetic negative selection followed by dielectrophoretic concentration for direct detection of genetic mutations in rare cancer cells suspended in whole blood.
An integrated multi-molecular sensor for simultaneous BRAFV600E protein and DNA single point mutation detection in circulating tumour cells
We report an integrated multi-molecular sensor (IMMS) platform for an entire sample-to-answer protocol encompassing melanoma cell capture in biological fluids, on-chip cell lysis, and combined quantification of intracellular BRAFV600E DNA and protein amounts.
A microfluidic platform integrated with field-effect transistors for enumeration of circulating tumor cells
A new integrated microfluidic system was developed for automatic detection and enumeration of CTCs with field-effect transistors (FETs).
Multilayer microfluidic array for highly efficient sample loading and digital melt analysis of DNA methylation
This microfluidic platform captures and analyzes rare molecules, such as cell-free DNA in liquid biopsies, to establish new epigenetic insights.
Rapid and efficient isolation and detection of extracellular vesicles from plasma for lung cancer diagnosis
A dielectrophoretic chip has been developed for extracellular vesicle (EV) isolation, which facilitates high-recovery efficiency (>83%) and high-purity EV isolation from plasma.
A high-throughput liquid biopsy for rapid rare cell separation from large-volume samples
The developed high-throughput liquid biopsy platform for rare tumor cell separation from body fluids has shown enormous promise in cancer detection and prognosis monitoring.
Urine-based liquid biopsy: non-invasive and sensitive AR-V7 detection in urinary EVs from patients with prostate cancer
Detection of AR-V7 in urinary EVs provides a simple and promising liquid biopsy tool for patients with prostate cancer.
Ultrasensitive quantification of tumor mRNAs in extracellular vesicles with an integrated microfluidic digital analysis chip
A microwell-patterned microfluidic digital mRNA analysis platform enables PCR-free, single-molecule detection of EWS-FLI1 fusion transcripts in EVs towards liquid biopsy-based non-invasive diagnosis of Ewing Sarcoma.
Acoustic impedance-based size-independent isolation of circulating tumour cells from blood using acoustophoresis
Here, we report a label-free method based on acoustic impedance contrast for the isolation of CTCs from peripheral blood mononuclear cells (PBMCs) in a microchannel using acoustophoresis. Applying this method, we demonstrate the label-free isolation of HeLa and MDA-MB-231 cells from PBMCs.
Versatile exclusion-based sample preparation platform for integrated rare cell isolation and analyte extraction
Automated magnetic bead sample preparation platform for positive/negative cell selection and molecular endpoints.
Label-free isolation of rare tumor cells from untreated whole blood by interfacial viscoelastic microfluidics
Label-free, size-dependent, and high-throughput isolation of rare tumor cells from untreated whole blood is enabled by interfacial viscoelastic microfluidics.
Microfluidic-based solid phase extraction of cell free DNA
Plastic microfluidic device for the efficient isolation (>90% recovery) of cell free DNA from plasma for molecular profiling.
Microfluidic affinity separation chip for selective capture and release of label-free ovarian cancer exosomes
An elution technique selectively captures and releases intact, label-free exosomes from a microfluidic device for characterizing ovarian cancer serum.
Effect of exercise on the plasma vesicular proteome: a methodological study comparing acoustic trapping and centrifugation
Improved reproducibility in seed particle mediated acoustic trapping of submicron particles enables clinical biomarker studies in extracellular vesicles.
Frequency-enhanced transferrin receptor antibody-labelled microfluidic chip (FETAL-Chip) enables efficient enrichment of circulating nucleated red blood cells for non-invasive prenatal diagnosis
We proposed a FETAL-Chip for efficient enrichment of cNRBCs, which offers great potential for NIPD.
Dynamic CTC phenotypes in metastatic prostate cancer models visualized using magnetic ranking cytometry
Magnetic ranking cytometry profiles dynamic phenotypes in CTCs that are linked to metastatic potential.
Isolation and genome sequencing of individual circulating tumor cells using hydrogel encapsulation and laser capture microdissection
Single CTC sequencing workflow using biophysical enrichment and single cell isolation by laser capture microdissection.
Fully automated, on-site isolation of cfDNA from whole blood for cancer therapy monitoring
Fully integrated lab-on-a-disc for cfDNA isolation allows real-time monitoring of tumor mutation status during targeted therapy.
Microfluidic isolation of platelet-covered circulating tumor cells
A new CTC isolation approach uncovers special populations of platelet-coated CTCs and CTC-leukocyte clusters in cancer patients.
A magnetic micropore chip for rapid (<1 hour) unbiased circulating tumor cell isolation and in situ RNA analysis
We have developed a microchip platform that combines fast, magnetic micropore based negative selection (>10 mL h−1) with rapid on-chip in situ RNA profiling.
Label-free ferrohydrodynamic cell separation of circulating tumor cells
A size-based ferrohydrodynamic cell separation (FCS) device capable of enriching intact circulating tumor cells with high throughput and high recovery rate.
Size-tunable microvortex capture of rare cells
A wide distribution of cell sizes can be selectively captured by tuning the flow rate in a micro-vortex flow.
About this collection
Liquid Biopsy, coined by Pantel and Alix-Panabières in 2010, originally referred to real-time analyses of CTCs in cancer. However, that term has since expanded to encompass the analyses of many other disease-related substances found in blood and other body fluids. Our goal is to highlight the new advances in this growing field with an emphasis on the interface between the technological advancements and high impact applications of liquid biopsy technologies. These would include manuscripts related to components that can be captured or characterized from blood such as circulating tumour cells, circulating nucleic acids and circulating extracellular vesicles. This on-going collection is collated by Thought Leaders Mehmet Toner and Stefanie Jeffrey and the Lab on a Chip Editorial Office.
More details about the collection and how to apply can be found at rsc.li/blog-liquid-biopsy