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Frequency-Enhanced Transferrin Receptor Antibody-Labelled Microfluidic Chip (FETAL-Chip) Enables Efficient Enrichment of Circulating Nucleated Red Blood Cells for Non-invasive Prenatal Diagnosis

Abstract

Fetal aneuploidy and other chromosomal aberrations affect 9 of 1000 live births. Unlike the invasive diagnosis with high risk of miscarriage, noninvasive prenatal diagnosis (NIPD) sampling from maternal blood becomes a promising way for fetal genetic screening. Unfortunately, the rare number of fetal cells present in the maternal blood remains a major challenge for fetal cell-based prenatal diagnosis. We designed a Frequency-Enhanced Transferrin receptor Antibody-Labelled Microfluidic Chip (FETAL-Chip) for efficient enrichment and identification of circulating fetal cells, i.e., circulating nucleated red blood cells (cNRBCs) from maternal blood. The FETAL-Chip can dramatically enhance the interaction of fetal cells with antibody-coated micro-posts to increase the capture efficiency while minimizing nonspecific adsorption. With the help of immunostaining, we can identify cNRBCs from as low as 2 milliliter maternal blood. Various numbers of cNRBCs were detected from volunteers as early as 7 weeks after conception and throughout the entire pregnancy. Gene analysis was also carried out to confirm the fetal origin of captured cells. With easy, noninvasive and highly efficient enrichment of cNRBCs, the method presented here offers great potential for noninvasive prenatal diagnosis.

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Publication details

The article was received on 26 Jun 2018, accepted on 06 Aug 2018 and first published on 09 Aug 2018


Article type: Paper
DOI: 10.1039/C8LC00650D
Citation: Lab Chip, 2018, Accepted Manuscript
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    Frequency-Enhanced Transferrin Receptor Antibody-Labelled Microfluidic Chip (FETAL-Chip) Enables Efficient Enrichment of Circulating Nucleated Red Blood Cells for Non-invasive Prenatal Diagnosis

    H. Zhang, Y. Yang, X. Li, Y. Shi, B. Hu, Y. An, Z. Zhu, G. Hong and C. J. Yang, Lab Chip, 2018, Accepted Manuscript , DOI: 10.1039/C8LC00650D

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