Themed collection Multimolecular Crowding in Biosystems

26 items
Highlight

Fluorescent probes for targeting endoplasmic reticulum: design strategies and their applications

Fluorescent molecules for monitoring endoplasmic reticulum and chemical probes for the detection of ER cellular processes and analytes.

Graphical abstract: Fluorescent probes for targeting endoplasmic reticulum: design strategies and their applications
From the themed collection: Multimolecular Crowding in Biosystems
Highlight

Reaction-based fluorescent and chemiluminescent probes for formaldehyde detection and imaging

The major developments of fluorescent and chemiluminescent formaldehyde probes have been summarized in this highlight.

Graphical abstract: Reaction-based fluorescent and chemiluminescent probes for formaldehyde detection and imaging
From the themed collection: Multimolecular Crowding in Biosystems
Feature Article

Small-molecule fluorescent probes: big future for specific bacterial labeling and infection detection

In the last decade, small-molecule fluorescent probes brightened the bacteria and infections.

Graphical abstract: Small-molecule fluorescent probes: big future for specific bacterial labeling and infection detection
From the themed collection: Multimolecular Crowding in Biosystems
Feature Article

Cancer diagnosis and analysis devices based on multimolecular crowding

The study of multimolecular crowding has opened up the possibility of developing new devices for cancer diagnosis and analysis.

Graphical abstract: Cancer diagnosis and analysis devices based on multimolecular crowding
From the themed collection: Multimolecular Crowding in Biosystems
Feature Article

Regulation of liquid–liquid phase separation with focus on post-translational modifications

This feature article introduces some regulators of biomolecular condensates formed through liquid–liquid phase separation (LLPS), especially post-translational modifications (PTMs).

Graphical abstract: Regulation of liquid–liquid phase separation with focus on post-translational modifications
From the themed collection: Multimolecular Crowding in Biosystems
Feature Article

Enzymatic noncovalent synthesis of peptide assemblies generates multimolecular crowding in cells for biomedical applications

This article highlights the unique features of enzymatic noncovalent synthesis (ENS) for generating multimolecular crowding in cells and the relevant applications for biomedicines.

Graphical abstract: Enzymatic noncovalent synthesis of peptide assemblies generates multimolecular crowding in cells for biomedical applications
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Review Article

Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches

In this review, we describe chemogenetics of cell surface receptors. This approach using designer ligands allows for rapid and selective control of the designer receptor function without affecting the endogenous systems.

Graphical abstract: Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
From the themed collection: Exploring proteins and their interactions
Open Access Review Article

Molecular probes for cellular imaging of post-translational proteoforms

Specific post-translational modification (PTM) states of a protein affect its property and function; understanding their dynamics in cells would provide deep insight into diverse signaling pathways and biological processes.

Graphical abstract: Molecular probes for cellular imaging of post-translational proteoforms
From the themed collection: Multimolecular Crowding in Biosystems
Communication

A fluorogenic probe targeting two spatially separated enzymes for selective imaging of cancer cells

We describe a fluorogenic probe BocLys(Ac)-AB-FC targeting both histone deacetylases (HDACs) and cathepsin L, which are overexpressed in spatially separated subcellular organelles of cancer cells.

Graphical abstract: A fluorogenic probe targeting two spatially separated enzymes for selective imaging of cancer cells
From the themed collection: Multimolecular Crowding in Biosystems
Communication

A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-D-aspartate (NMDA) receptors

In this article, we report coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties as the first reversible fluorescent probe that can monitor GSH near NMDA receptors.

Graphical abstract: A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-d-aspartate (NMDA) receptors
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Intracellular photocatalytic-proximity labeling for profiling protein–protein interactions in microenvironments

Intracellular photocatalytic-proximity labeling (iPPL) was developed to profile protein–protein interactions in the microenvironment of living cells.

Graphical abstract: Intracellular photocatalytic-proximity labeling for profiling protein–protein interactions in microenvironments
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Development of 1,8-naphthalimide dyes for rapid imaging of subcellular compartments in plants

We report the installation of 1,8-naphthalimide dyes in live cell imaging of plants. The structure of the chloroplasts or vacuole was rapidly and clearly visualized by 1,8-naphthalimide dyes.

Graphical abstract: Development of 1,8-naphthalimide dyes for rapid imaging of subcellular compartments in plants
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Tumour enzyme affinity mediated peptide molecular crowding for targeted disruption of hyperactivated glucose uptake

An unconventional environment-responsive molecular crowding via specific binding between small molecule peptide inhibitor derivatives and overexpressed tumour enzyme has been developed.

Graphical abstract: Tumour enzyme affinity mediated peptide molecular crowding for targeted disruption of hyperactivated glucose uptake
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Communication

Bottom-up/cross-linking mass spectrometry via simplified sample processing on anion-exchange solid-phase extraction spin column

Capturing proteins on anion-exchange discs facilitates concentration of diluted samples and removal of contaminants, allowing more efficient sample pretreatment for bottom-up/cross-linking mass spectrometry than in-solution and in-gel.

Graphical abstract: Bottom-up/cross-linking mass spectrometry via simplified sample processing on anion-exchange solid-phase extraction spin column
From the themed collection: Multimolecular Crowding in Biosystems
Communication

ROS activated prodrug for ALDH overexpressed cancer stem cells

We developed a prodrug (DE-CPT) that efficiently decreases the cancer stem cell population and kills the cancer cells by ROS activation.

Graphical abstract: ROS activated prodrug for ALDH overexpressed cancer stem cells
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene

We identified cytosine-rich regions adjacent to guanine-rich regions in the TMPRSS2 gene, which showed structural competition between a G-quadruplex and a hairpin loop. Furthermore, this competition significantly affected transcription efficiency.

Graphical abstract: Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

Novel polynorbornene-based probes for fluorescence sensing of ATP at nanomolar levels via the indicator displacement assay (IDA) method are reported.

Graphical abstract: Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Communication

Harnessing stress granule formation by small molecules to inhibit the cellular replication of SARS-CoV-2

Small-molecule enhancers of cellular stress granules were identified by observing molecular crowding of proteins and RNAs in a time-dependent manner. Hit molecules inhibited the replication of SARS-CoV-2 by inducing stress granule formation.

Graphical abstract: Harnessing stress granule formation by small molecules to inhibit the cellular replication of SARS-CoV-2
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Calix[n]triazolium based turn-on fluorescent sensing ensemble for selective adenosine monophosphate (AMP) detection

We report a series of calix[n]triazoliums showing excellent selectivity for AMP. The unique supramolecular ensemble exhibits a selective turn-on fluorescence response towards AMP over various anions, including adenosine polyphosphates.

Graphical abstract: Calix[n]triazolium based turn-on fluorescent sensing ensemble for selective adenosine monophosphate (AMP) detection
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Metabolic iron detection through divalent metal transporter 1 and ferroportin mediated cocktail fluorogenic probes

A cocktail [1 + 2] dual-fluorescent probe system could realize the real-time visualization of dynamic iron state changes between Fe2+ and Fe3+.

Graphical abstract: Metabolic iron detection through divalent metal transporter 1 and ferroportin mediated cocktail fluorogenic probes
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Distinct impact of glycation towards the aggregation and toxicity of murine and human amyloid-β

Modifications of mAβ and hAβ by glycation can differentiate their aggregation and cytotoxicity profiles.

Graphical abstract: Distinct impact of glycation towards the aggregation and toxicity of murine and human amyloid-β
From the themed collection: Multimolecular Crowding in Biosystems
Communication

Nongenetic control of receptor signaling dynamics using a DNA-based optochemical tool

We demonstrated the nongenetic temporal control of cell signaling using a DNA-based synthetic surrogate of growth factor.

Graphical abstract: Nongenetic control of receptor signaling dynamics using a DNA-based optochemical tool
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Paper

Molecular design of near-infrared (NIR) fluorescent probes targeting exopeptidase and application for detection of dipeptidyl peptidase 4 (DPP-4) activity

We developed a new molecular design for NIR fluorescent probes that target exopeptidase by utilizing the >110 nm blueshift of unsymmetrical Si–rhodamines.

Graphical abstract: Molecular design of near-infrared (NIR) fluorescent probes targeting exopeptidase and application for detection of dipeptidyl peptidase 4 (DPP-4) activity
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Paper

Embedding a membrane protein into an enveloped artificial viral replica

We demonstrate the embedding membrane protein, Cx43, on the enveloped artificial viral capsid using a cell-free expression system. The embedding of Cx43 on the envelope was evaluated by detection with anti-Cx43 antibody using FCS and TEM.

Graphical abstract: Embedding a membrane protein into an enveloped artificial viral replica
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Paper

Versatile naphthalimide tetrazines for fluorogenic bioorthogonal labelling

New naphthalimide tetrazine probes permit fluorescent imaging of biomolecules in vitro and in living cells. They can be modified to provide previously unknown information about health and disease in biological systems.

Graphical abstract: Versatile naphthalimide tetrazines for fluorogenic bioorthogonal labelling
From the themed collection: Multimolecular Crowding in Biosystems
Open Access Paper

Lysine succinylation on non-histone chromosomal protein HMG-17 (HMGN2) regulates nucleosomal DNA accessibility by disrupting the HMGN2–nucleosome association

Succinylated HMGN2, prepared by a ‘thiol–ene reaction’, disrupted the association of HMGN2 with the nucleosome and increased nucleosomal DNA accessibility.

Graphical abstract: Lysine succinylation on non-histone chromosomal protein HMG-17 (HMGN2) regulates nucleosomal DNA accessibility by disrupting the HMGN2–nucleosome association
From the themed collection: Exploring proteins and their interactions
26 items

About this collection

Living cells comprise a variety of molecules from small cations/anions, metabolites, lipids, peptides, to biopolymers such as peptides/proteins, polysaccharides, and nucleic acids. These biomolecules are highly condensed inside the live cells and their distribution and concentration are heterogeneously varied (in a controlled manner). Such multimolecular crowding conditions are sharply distinct from those of conventional biochemical study, should be crucial for elucidating structures and functions of biomolecules in live cells, as well as for developing functional artificial molecules useful in chemical biology study, drug discovery, and medical diagnosis.

In this cross-journal themed issue, explore research papers, reviews, and communications in ChemComm and RSC Chemical Biology concerning chemical, physical, theoretical, or biological characterization of various biomolecules under the multimolecular crowding conditions. Also discover the development of chemical tools and devices enabling the detection and imaging of key biomolecules and the relevant events in live cells, methods for the structural modification and functional switching/control of biomolecules, and analytical or physical chemistry approaches applicable to study of multimolecular crowding biosystems.


Spotlight

Advertisements