Ling-Xi Huang, Yue-Bo He, Jaewon Kim, Amit Sharma, Qian-Yong Cao and Jong Seung Kim
Chem. Commun., 2021,57, 13530-13533
DOI:
10.1039/D1CC05500C,
Communication
An indicator displacement assay, namely polymeric PNPY-n/UD consisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive “turn-on” fluorescence sensing of ATP. While PNPY-1/UD itself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [Ka = 2.17 × 105 M−1, LOD = 5.7 nM]. The potential of a highly photostable system PNPY-1/UD was also validated in detecting ATP levels in live-cell imaging applications.