Themed collection Emerging Investigators
Inside front cover
Inside back cover
Emerging investigators 2015
Welcome to this Analytical Methods themed issue on emerging investigators. Lane Baker and Ryan Bailey discuss the work showcased.
Contributors to the emerging investigators issue
Analytical Methods profiles the emerging investigators featuring work in this themed issue.
Analytical chemistry research at primarily undergraduate institutions: training tomorrow's investigators
This perspective presents challenges, benefits, and recent advances from research at primarily undergraduate institutions.
Recent developments in electrochemical paper-based analytical devices
This mini-review discusses recent advances in electrochemical paper-based analytical devices with an emphasis on applications of traditional electrochemical methods.
Monitoring of chemical transformations by mass spectrometry
During the last several decades, mass spectrometry (MS) has rapidly developed as a practical technique that can be used to monitor chemical reactions and investigate reaction mechanisms.
Multimodal scanning probe imaging: nanoscale chemical analysis from biology to renewable energy
Multimodal scanning probe microscopy enables analysis of not only surface topography, but also surface chemistry, electrical, magnetic, and mechanical properties.
Electrochemical nanoprobes for the chemical detection of neurotransmitters
Neurotransmitters, acting as chemical messengers, play an important role in neurotransmission, which governs many functional aspects of nervous system activity.
A review of traditional and emerging methods to characterize lipid–protein interactions in biological membranes
Lipid–protein interactions are essential for modulating membrane protein structures and biological functions in the cell plasma membrane. In this review we describe the salient features of classical and emerging methodologies for studying protein–lipid interactions and their limitations.
Photoluminescent nanosensors for intracellular detection
Main strategies for detecting changes in intracellular parameters (pH, ion concentration, reactive oxygen species, temperature, RNA levels and enzymatic activity) with photoluminescent nanosensors are reviewed in detail.
Emergence of tunable resistive pulse sensing as a biosensor
A review and tutorial on tunable resistive pulse sensors used as biosensors.
DNA–bare gold affinity interactions: mechanism and applications in biosensing
Sequence-dependent non-thiolated DNA–gold affinity interaction for biosensing applications, and the underlying principles governing these interactions are comprehensively reviewed.
Supermacroporous polyHIPE and cryogel monolithic materials as stationary phases in separation science: a review
With their unique supermacroporous architecture, polyHIPEs (high internal phase emulsions) and cryogels have huge potential as analytical separation stationary phases.
Emerging techniques for the in situ analysis of reaction intermediates on photo-electrochemical interfaces
We offer a perspective on how new in situ methods enable the chemically-sensitive, time-resolved and spatially-resolved exploration of semiconductor surface photo(electro)chemistry.
Tracking single particle rotation: probing dynamics in four dimensions
Direct visualization and tracking of small particles at high spatial and temporal resolution provides a powerful approach to probing complex dynamics and interactions in chemical and biological processes.
Combined electrochemical-topographical imaging: a critical review
This review critically analyses the state-of-the-art in correlative electrochemical-topographical imaging, focusing on AFM, shear-force, ion conductance, and electrochemical positional feedback.
Sensing in aqueous medium: mechanism and its application in the field of molecular recognition
Chemosensor development and their mechanisms are detailed for molecular recognition.
Electron capture dissociation of extremely supercharged protein ions formed by electrospray ionisation
Extreme supercharging of proteins yields significant performance gains for the direct characterization of protein sequences by electron capture dissociation mass spectrometry.
Microfluidic protein crystallisation controlled using spontaneous emulsification
Microdroplet-based protein crystallisation using spontaneous emulsification is proposed and demonstrated. The dependency of crystal number in a single microdroplet on the surfactant concentration is discussed.
Single-cell migration as studied by scanning electrochemical microscopy
An SECM based analytical method to study single-cell biomechanics is reported to differentiate between migrating and stationary cancer cells.
Quantifying aptamer–protein binding via thermofluorimetric analysis
Homogeneous, aptamer-based protein quantification via thermofluorimetric analysis (TFA) permits subtraction of autofluorescence in biological samples.
Microelemental characterisation of Aboriginal Australian natural Fe oxide pigments
This manuscript presents the first comprehensive microcharacterisation of Fe oxide minerals used in Aboriginal Australian mineral pigments.
Quantitation of thiol metabolites from mammalian cells using fluorous tagging and HILIC-MS
Oxidative stress diminishes reduced thiols by decreasing their production or by forming disulfide bonds.
A density-controlled scaffolding strategy for covalent functionalization of carbon-fiber microelectrodes
We describe a covalent functionalization method for creating density-controlled selective adsorption sites on carbon-fiber microelectrodes.
Multiplexing strategy for simultaneous detection of redox-, phospho- and total proteome – understanding TOR regulating pathways in Chlamydomonas reinhardtii
A multiplexing 2DE-based approach for parallel analysis of redox-, phospho- and total-proteome is presented and applied to the study of Chlamydomonas TOR pathway.
Exploratory analysis of avocado extracts by GC-MS: new insights into the avocado fruit ripening process
Herewith the usefulness of a gas chromatography-electron ionization-quadrupole mass spectrometry (GC-EI-Q MS) methodology has been evaluated for the identification of potential ripening or variety markers of the avocado fruit.
Rapid determination of multiclass fungicides in wine by low-temperature plasma (LTP) ambient ionization mass spectrometry
LTP-MS/MS is a rapid method for the qualitative and semi-quantitative determination of pesticides in wines.
Towards improving the robustness of electrochemical gas sensors: impact of PMMA addition on the sensing of oxygen in an ionic liquid
An ionic liquid–polymer gel was found to improve the robustness of an integrated planar electrode system for portable oxygen gas-sensing applications.
Colorimetric determination of nitrite in clinical, food and environmental samples using microfluidic devices stamped in paper platforms
Paper microfluidic devices were explored to perform the colorimetric determination of nitrite in saliva, sausage, ham, preservative water and river water samples with no statistical difference from a reference method.
Fluorescent microRNA biosensors: a comparison of signal generation to quenching
Many microRNA biosensor platforms regard fluorescence signal generation as superior to quenching; however, the two methods are comparable rather than competitive.
Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture
Cell collection based on deterministic lateral displacement (DLD) and cell circulation with a loop channel are two component technologies for stressless cell retention which have been developed with a view to working toward suspension culture in a microfluidic channel.
Multiplexed single-cell in situ RNA analysis by reiterative hybridization
A novel method to quantify the identities, positions, and copy numbers of a large number of different RNA species in single cells has been developed by reiterative cycles of target hybridization, fluorescence imaging and photobleaching.
A spectral sieve-based strategy for sensing inorganic and organic traces on solid surfaces using laser-induced breakdown spectroscopy
Profiting the correlation between emission signals to detect residues on solid surfaces when laser ablation faces inspection of suspicious targets.
Self-assembly pipette tip-based cigarette filters for micro-solid phase extraction of ketoconazole cis-enantiomers in urine samples followed by high-performance liquid chromatography/diode array detection
A simple stereoselective HPLC/DAD method was developed for the determination of ketoconazole (KTZ) cis-enantiomers in human urine.
Rapid screening of antibody–antigen binding using dynamic light scattering (DLS) and gold nanoparticles
Antigen-mediated aggregation of antibody–gold nanoparticle conjugates is detected with dynamic light scattering to evaluate antibody–antigen binding.
A magnetically tuneable piezoresistive sensor for direct, in situ strain measurements in Li-ion batteries
Strain from Li-alloying anodes in operando is directly detected using generic battery components as a piezoresistive sensor. Magnetic-field alignment effectively enhances the sensitivity of this sensor.
Analysis of fluid film behaviour using dynamic wetting at a smooth and roughened surface
The dynamic wetting technique is described and used to create and aid in analysis of ultrathin (1–5 nm) films on vertically aligned, planar silver substrates of varying microscopic roughness (RMS roughness between 1 and 7 nm).
Site-selective characterization of Src homology 3 domain molecular recognition with cyanophenylalanine infrared probes
We present site-selective CNPhe labeling combined with FT IR spectroscopy as a fast, minimally-perturbative, reproducible approach to characterize protein microenvironments.
Enhancing the mass spectrometric identification of membrane proteins by combining chemical and enzymatic digestion methods
Combining chemical and enzymatic digestion methods proved to be extremely effective for mass-spectrometry (MS)-based membrane protein analysis.
Monitoring the clandestine synthesis of methamphetamine in real-time with ambient sampling, portable mass spectrometry
In this paper, an ambient sampling, portable mass spectrometer coupled to a selection on ambient ionization methods was used to perform real-time reaction monitoring of clandestine methamphetamine syntheses.
Green detection of the olive fruit fly pest by the direct determination of its sexual pheromone
Bactrocera oleae Gmelin, the olive fruit fly, is considered a serious pest in the cultivation of olive trees since the larvae feed on the fruit damaging the productivity and quality of the final products (olive fruit and olive oil).
Chemometric analysis of MALDI mass spectrometric images of three-dimensional cell culture systems
As imaging mass spectrometry (IMS) has grown in popularity in recent years, the applications of this technique have become increasingly diverse.
Investigating optimum sample preparation for infrared spectroscopic serum diagnostics
Biofluids, such as serum and plasma, represent an ideal medium for disease diagnosis due to their non-invasive character, ease of collection, that can be performed worldwide, and their fundamental involvement in human functions.
Analysis of bacterial plasmid DNA by solid-phase microextraction
A polymeric ionic liquid sorbent coating is applied in the extraction of two different plasmids from a mixture of E. coli transformants using solid-phase microextraction.
Food safety: how analytical chemists ensure it
Nowadays consumer concerns related to food safety are increasing and analytical chemists should provide suitable analytical tools to fulfil international legislation and ensure that foodstuff is adequate to be consumed.
A strategy for minimizing background signal in autoinductive signal amplification reactions for point-of-need assays
This paper describes a small molecule signal amplification reagent and an assay strategy that incorporates in situ signal amplification with minimal production of background signal.
A non-destructive test to assess the axial heterogeneity of in situ modified monoliths for HPLC
A multi-location peak parking protocol was developed for a non-destructive assessment of the axial heterogeneity of in situ modified monoliths. This was tested on a column with a surface coverage density gradient along the length of the monolithic rod. Qualitative changes in band broadening were observed and were consistent with theoretical studies.
Improved monitoring of P. aeruginosa on agar plates
A disposable electrochemical assay that is integrated with standard culture plates for the selective and specific detection of Pseudomonas aeruginosa.
Ultrasound-assisted digestion of biodiesel samples for determination of metals by stripping voltammetry
Room-temperature ultrasound-assisted digestion of biodiesel for stripping voltammetry of metals with adequate recovery values (94–108%) and low residual carbon content is demonstrated.
About this collection
This is a collection of papers consisting of invited contributions including perspectives, reviews, and original research articles from emerging investigators in the analytical sciences. Guest Edited by Lane Baker and Ryan Bailey. New articles will be added to this collection as they are published.