Jump to main content
Jump to site search

Issue 17, 2015
Previous Article Next Article

Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

Author affiliations

Abstract

Cell collection based on deterministic lateral displacement (DLD) and cell circulation with a loop channel are two component technologies for stressless cell retention which have been developed with a view to working toward suspension culture in a microfluidic channel. DLD devices with low array angles collect floating S. cerevisiae through an array effectively. The DLD device with an array angle of 2.6° showed an efficiency of 91.7%. Two types of loop channels with a piezoelectrie pump make a stable two-phase laminar flow of a pre-filled fluid and supplied fluid. A loop channel with a connection between an inlet and outlet on the opposite side replaces a filled fluid in the channel with a supplied fluid, which is essential for supplying nutrient rich medium to cells in microfluidic channels as well as retaining cells in a microenvironment without external stresses.

Graphical abstract: Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

Back to tab navigation

Supplementary files

Article information


Submitted
24 Feb 2015
Accepted
14 Apr 2015
First published
06 May 2015

Anal. Methods, 2015,7, 7264-7269
Article type
Paper

Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

T. Naito, N. Kaji, M. Tokeshi, T. Kubo, Y. Baba and K. Otsuka, Anal. Methods, 2015, 7, 7264
DOI: 10.1039/C5AY00485C

Social activity

Search articles by author

Spotlight

Advertisements