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Issue 17, 2015
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Microfluidic protein crystallisation controlled using spontaneous emulsification

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Abstract

Microdroplet-based protein crystallisation using spontaneous emulsification is proposed and demonstrated. The dependence of water transport on the concentration of Span 80 was investigated. Crystallisation of the lysozyme was then demonstrated. Based on the results obtained, the dependency of the crystal number in a single microdroplet on the surfactant concentration was discussed.

Graphical abstract: Microfluidic protein crystallisation controlled using spontaneous emulsification

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Publication details

The article was received on 05 Mar 2015, accepted on 15 Apr 2015 and first published on 16 Apr 2015


Article type: Communication
DOI: 10.1039/C5AY00578G
Anal. Methods, 2015,7, 7128-7131
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    Microfluidic protein crystallisation controlled using spontaneous emulsification

    M. Fukuyama, A. Akiyama, M. Harada, T. Okada and A. Hibara, Anal. Methods, 2015, 7, 7128
    DOI: 10.1039/C5AY00578G

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