Themed collection Probes for in vitro and in vivo fluorescence imaging
Carbon-based hybrid nanogels: a synergistic nanoplatform for combined biosensing, bioimaging, and responsive drug delivery
This review summarizes the latest developments and addresses current applications and future perspectives of carbon-based hybrid nanogels in the biomedical field.
Peptide-based nanoprobes for molecular imaging and disease diagnostics
Peptide-based nanoprobes can improve the diagnosis, staging, treatment, management, and prognosis of numerous diseases by interfacing with and/or sensing of disease-relevant biomolecules.
Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications
Peptide– and protein–nanoparticle conjugates have emerged as powerful tools for biomedical applications, enabling the treatment, diagnosis, and prevention of disease.
Ratiometric optical nanoprobes enable accurate molecular detection and imaging
Conventional optical nanoprobes rely on absolute intensity-dependent signal readout, which can often cause erroneous sensing and imaging results owing to the existence of analyte-independent confounding factors. Ratiometric signal processing provides built-in self-calibration for signal correction, enabling more accurate molecular detection and imaging.
Optical nano-biosensing interface via nucleic acid amplification strategy: construction and application
This article reviews recent smart constructions and applications of optical nano-biosensing interfaces assisted by a nucleic acid amplification strategy.
Engineering functional inorganic–organic hybrid systems: advances in siRNA therapeutics
Engineering functional inorganic–organic hybrid vectors for siRNA delivery systems as a promising approach to overcome many physiological and biological obstacles, which greatly paves the way for a successful, safe and efficient platform in clinical applications.
Designed transition metal catalysts for intracellular organic synthesis
A review of progress, challenges, and future prospects in developing transition metal catalysts for intracellular organic synthesis.
Far-red fluorescent probes for canonical and non-canonical nucleic acid structures: current progress and future implications
Our review presents the recent progress on far-red fluorescent probes of canonical and non-canonical nucleic acid (NA) structures, critically discusses the design principles, applications, limitations and outline the future prospects of developing newer probes with target-specificity for different NA structures.
Calcium-based biomaterials for diagnosis, treatment, and theranostics
Calcium-based biomaterials with good biosafety and bio-absorbability are promising for biomedical applications such as diagnosis, treatment, and theranostics.
Fluorescent probes for the simultaneous detection of multiple analytes in biology
This review identifies and discusses fluorescent sensors that are capable of simultaneously reporting on the presence of two analytes for biological application.
The development of ruthenium(II) polypyridyl complexes and conjugates for in vitro cellular and in vivo applications
A detailed overview of the development of Ru(II) polypyridyl complexes as diagnostic and therapeutic agents in vitro and in vivo.
Chemistry and engineering of cyclodextrins for molecular imaging
Cyclodextrins (CDs) are naturally occurring cyclic oligosaccharides that show favourable properties for probe design and development.
Hybridization chain reaction: a versatile molecular tool for biosensing, bioimaging, and biomedicine
This review provides a comprehensive overview of the fundamental principles, analysis techniques, and application fields of hybridization chain reaction and its development status.
Recent progress in surface-enhanced Raman spectroscopy for biological and biomedical applications: from cells to clinics
In this tutorial review, we summarize the recent progress in SERS-based biological and biomedical applications – from cells to clinics.
Development of endogenous enzyme-responsive nanomaterials for theranostics
This review summarizes the recent progress of endogenous enzyme-responsive nanomaterials based on different building blocks such as polymers, liposomes, small organic molecules, or inorganic/organic hybrid materials for theranostics.
Crucial breakthrough of second near-infrared biological window fluorophores: design and synthesis toward multimodal imaging and theranostics
Recent advances in the chemical design and synthesis of fluorophores in the second near-infrared biological window (NIR-II) for multimodal imaging and theranostics are summarized and highlighted in this review article.
A guide to integrating immunohistochemistry and chemical imaging
A ‘how-to’ guide for designing chemical imaging experiments using antibodies and immunohistochemistry.
Shedding light on tau protein aggregation: the progress in developing highly selective fluorophores
The development of highly selective fluorophores for tau protein aggregates, a key feature of Alzheimer's disease, is highlighted.
Quinone-based fluorophores for imaging biological processes
This tutorial review covers recent advances in the design, synthesis and application of quinone-based fluorescent agents for visualizing specific processes in multiple biological systems, from cells to tissues and complex organisms in vivo.
Fluorogenic reaction-based prodrug conjugates as targeted cancer theranostics
This Tutorial Review will describe various fluorogenic reaction-based prodrug strategies used for targeted theranostic drug delivery.
Fluorescent chemosensors: the past, present and future
Fluorescent chemosensors for ions and neutral analytes have been widely applied in many diverse fields such as biology, physiology, pharmacology, and environmental sciences.
Multiplexed photoluminescent sensors: towards improved disease diagnostics
This tutorial review highlights the development of multiplexed photoluminescent sensors which can simultaneously detect multiple and diverse biomarkers that exist in a homogenous solution or a single cell, accelerating the progress towards precise disease diagnostics.
Advancing porphyrin's biomedical utility via supramolecular chemistry
Enhancing existing porphyrin functionality and introducing new capabilities via supramolecular chemistry.
Supramolecular catalysis and dynamic assemblies for medicine
This review provides new insights into supramolecular catalysis and dynamic regulation of molecular assemblies for biomedical applications.
Motion-induced change in emission (MICE) for developing fluorescent probes
A new concept of motion-induced change in emission (MICE) in a single molecule for developing fluorescent probes is presented and summarized.
Methods for the detection and identification of pathogenic bacteria: past, present, and future
In this tutorial review, the techniques involved in the detection of pathogenic bacteria are described.
About this collection
We are delighted to present a Chemical Society Reviews themed collection on probes for in vitro and in vivo fluorescence imaging. This collection brings together reviews from leading experts and details recent advances in this field, including fluorescence- and peptide-based nanoprobes, surface-enhanced Raman spectroscopy, molecular imaging and theranostics. We hope you enjoy reading these Reviews!