Themed collection Analytical Methods HOT Articles 2022
Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification
A new system termed droplet DNAzyme-coupled rolling circle amplification for single bacteria detection is presented.
Highly-fluorescent carbon dots grown onto dendritic silica nanospheres for anthrax protective antigen detection
Highly-fluorescent carbon dots grown onto dendritic silica nanospheres for immunochromatography to detect anthrax protective antigen.
Determination and quantification of related substances and degradation products in bictegravir by full factorial design evaluated HPLC and mass spectrometry
Determining and quantifying novel impurities and degraded impurities of a drug product is always a continuous challenge to enhancing the drug quality for patients' safety.
Study on direct identification of bacteria by laser-induced breakdown spectroscopy
A method of quickly and directly identifying bacteria species using LIBS is proposed. It uses filtering, normalization and PCA to preprocess the spectra, uses SVM with one-against-all linear kernel function to establish the prediction model.
Molecularly imprinted polymers as effective capturing receptors in a pseudo-ELISA immunoassay for procalcitonin detection in veterinary species
Pseudo-ELISA innovative assay based on the combined use of biomimetic receptors (MIP) and antibodies for procalcitonin detection.
A rhodamine based chemodosimeter for the detection of Group 13 metal ions
Group 13 cations induce hydrolysis of a rhodamine based dye to cause a sharp color change from colorless to pink and a high increment in fluorescence.
Fluorescent probes based on the core–shell structure of molecular imprinted materials and gold nanoparticles for highly selective glutathione detection
Glutathione fluorescent probes based on core–shell structures containing fluorescent dyes, molecularly imprinted materials, and gold nanoparticles are suitable for glutathione sensing in complex materials (such as serum).
pH-Responsive epitope-imprinted magnetic nanoparticles for selective separation and extraction of chlorogenic acid and caffeic acid in traditional Chinese medicines
By using caffeic acid as the epitope template of chlorogenic acid, the prepared epitope-imprinted MNPs could be applied to the selective separation and extraction of chlorogenic acid and caffeic acid in traditional Chinese medicines.
Covalent organic framework linked with amination luminol derivative as enhanced ECL luminophore for ultrasensitive analysis of cytochrome c
COFs were grafted with ABEI and applied for building a label-free ECL biosensor for cyt c detection.
Rapid authentication of coffee bean varieties of different forms by using a pocket-sized spectrometer and multivariate data modelling
Coffee is the most consumed beverage and the second most valuable traded commodity in the world.
Rapid and reliable detection and quantification of organophosphorus pesticides using SERS combined with dispersive liquid–liquid microextraction
SERS was combined with DLLME to develop a rapid and reliable method for the detection and quantification of organophosphorus pesticides, which exhibited good sensitivity and stability for both single and multiple detection of OPPs.
Rapid and highly selective colorimetric detection of mercury(II) ions in water sources based on a ribavirin functionalized AuNP sensor
Ribavirin-functionalized AuNP sensors would rapidly change from red to gray when Hg2+ was added, and could be applied to detect trace Hg2+ with fine selectivity and anti-interference in drinking water.
Identification of agricultural quarantine materials in passenger's luggage using ion mobility spectroscopy combined with a convolutional neural network
A new method for identification of AQMs in passenger's luggage using IMS combined with CNN.
Zeolitic imidazolate framework-8 modified magnetic halloysite nanotube-based solid phase extraction for the analysis of carbamate pesticides by ultra-high performance liquid chromatography tandem mass spectrometry
MHNTs@ZIF-8 based mSPE-UHPLC-MSMS for the analysis of carbamate pesticides in water.
A batch processed titanium–vanadium oxide nanocomposite based solid-state electrochemical sensor for zeptomolar nucleic acid detection
A bi-electrode sensor with a transition metal oxide-based active layer for electrochemical DNA/RNA amplification detection is developed. The device offers sensitivity towards 102 and 101 copies of SARS-CoV-2 plasmid and RNA, respectively.
A metal–organic framework loaded paper-based chemiluminescence sensor for trace acetone detection in exhaled breath
A paper-based CL gas sensor was established for sensitive detection of trace acetone in exhaled breath. UiO-66 with an adsorption function was in situ synthesized on the paper-based device.
Surface charge modulation enhanced high stability of gold oxidation intermediates for electrochemical glucose sensors
Gold nanoparticles were loaded on metal foam substrates by simple displacement reactions and applied to glucose sensors. The surface charge regulation between Au and Cu stabilized AuOH active site, thus enhanced the glucose sensing performance.
Polyethyleneimine-capped copper nanoclusters for detection and discrimination of 2,4,6-trinitrotoluene and 2,4,6-trinitrophenol
PEI-Cu NCs are successfully obtained for the detection and discrimination of TNT and TNP. Benefitting from excellent sensitivity, high specificity and rapid response time, they are expected to complement large equipment in chemical labs.
Selective and ultrasensitive citric acid assay in urine samples using a MWCNT/Fe3O4 nanoparticle-modified carbon paste electrode
A simple method for selective and ultrasensitive determination of citric acid using a CNT/Fe3O4-CPE and differential pulse voltammetry was introduced.
A fluorescent and scattering dual-mode probe based on a carbon dot@cerium–guanosine monophosphate coordination polymer network for tetracycline detection
Dual-mode sensing with a two-signal read-out is conducive to the improvement of detection accuracy.
Smartphone-based digital image colorimetry for determination of iron in cereals and crispy seaweed using Terminalia chebula retz. extract as a natural reagent
The workflow schematic of the proposed sample preparation procedure for iron(III) determination in whole wheat bread, granola, and crispy seaweed samples.
Reactive argon-plasma activation of screen-printed carbon electrodes for highly selective dopamine determination
A facile argon (Ar) plasma treatment was employed to enhance the electrocatalytic reactivity of screen-printed carbon electrodes for selective dopamine (DA) detection.
Development of monoclonal antibodies for the detection of diuron in water and sugarcane and their application in immunochromatographic strips
Diuron (DR) as a chemical herbicide that persists in soil and water for a long time and causes extensive harm to humans.
Chromatographic analysis of bromhexine and oxytetracycline residues in milk as a drug analysis medium with greenness profile appraisal
TLC densitometric and RP-HPLC methods for the determination of oxytetracycline and bromhexine with the sensitivity required for their analysis in milk samples accompanied by green profile appraisal.
Establishment of fumonisin B1 detection method for catalytic fluorescence detection of aptamer-regulated carbon dots
develop aptamer-regulated CDs for detection of FB1 by catalytic fluorescence. 2,2′-Dithiosalicylic acid was used to prepare carbon dots catalysing the fluorescence of TMB. Aptamer F10 can bind to FB1, restoring the catalytic capacity of CDs and increasing fluorescence.
Batch analysis of microplastics in water using multi-angle static light scattering and chemometric methods
Light scatterometry combined with chemometrics can be a practical approach for the analysis of size and concentration of microplastics in water.
Colloidal Au sphere and nanoflower-based immunochromatographic strips for sensitive detection of zearalenone in cereals
The AuNFs and AuSPs were successfully synthesized for labeling antibodies. The AuSP-ICS and AuNF-ICS were successfully fabricated for sensitive ZEN detection. The AuNF-ICS exhibited satisfactory sensitivity and specificity.
Synthesis and validation of ultrasensitive stripping voltammetric sensor based on polypyrrole@ZnO/Fe3O4 core–shell nanostructure for picomolar detection of artesunate and dopamine drugs
A stripping voltammetric sensor for ultrasensitive detection of artesunate (ART) and dopamine HCl (DA) has been successfully developed using a Ppy@ZnO/Fe3O4 core–shell nanocomposite ([PZM])-modified carbon paste sensor (MCPS).
Carbon dot-functionalized macroporous adsorption resin for bifunctional ultra-sensitive detection and fast removal of iron(III) ions
Heavy metal pollution has spread around the world with the development of industry, posing a major threat to human health.
Preparation of a glucose-sensitive one-dimensional photonic crystal via top–down nanocasting
We prepared a one-dimensional (1-D) photonic crystal gel sensor. It has great application potential in the preparation of point-of-care (POC) sensors.
One step cascade detection of galactose based on a galactose oxidase-composited peroxidase nanozyme
A novel one-step cascade method for galactose detection in neutral conditions was proposed by using a dual-active complex enzyme, which is composed of galactose oxidase and Cu-MOF nanozyme.
Speciation analysis of both inorganic and organic 129I in seawater and its application in the study of the marine iodine cycle
A complete protocol is presented for the speciation analysis of 129I for both inorganic and organic iodine in seawater using coprecipitation and solid-phase extraction (SPE) combined with accelerator mass spectrometry (AMS).
Enhanced detection of ascorbic acid with cascaded fluorescence recovery of a dual-nanoquencher system
An innovative fluorescence method has been proposed for enhanced detection of AA based on target-triggered cascade fluorescence recovery of a dual-nanoquencher system.
Characterization and predictive modeling potential of aging time of roasted coffee using infrared spectroscopy
FTIR-ATR and logistic regression model estimating aging time (shelf life) and detecting frauds of repacking or tampering with labels of roasted coffee.
Preparation of tannic acid and L-cysteine functionalized magnetic composites for synergistic enrichment of N-glycopeptides followed by mass spectrometric analysis
MTC was prepared and applied to specificly enrich N-glycopeptides based on the synergistic hydrophilic interaction between TA/L-Cys and N-glycopeptides.
Energy conversion efficiency comparison of different aqueous and semi-aqueous CO2 electroreduction systems
The characteristics of CO, HCOOH, C2H4, and CH4 production from CO2 electroreduction were analyzed by introducing the energy conversion efficiency as a new evaluation standard for electrocatalytic reactions.
Nontargeted metabolomics coupled with multivariate modelling techniques for discrimination of Cyclocarya paliurus (Batal.) Ijinskaja leaves from different geographic altitudes
Altitude-associated nutrition-compositional evaluation is critical for quality control and value determination of plants.
A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells
A 3D-printed multi-modal device was developed to simultaneously detect nitric oxide (NO) and adenosine triphosphate (ATP) in red blood cell suspensions.
TiO2@MOF-919(Fe–Cu) as a sorbent for the extraction of benzoylurea pesticides from irrigation water and fruit juices
The TiO2@MOF-919(Fe–Cu) solid-phase extraction material was prepared by growing MOF-919(Fe–Cu) in situ on three-dimensional radial TiO2 microspheres by a simple solvothermal method.
A novel simultaneous isotope dilution strategy as a powerful tool in the two-track certification process of trace metal mass fractions: a case study of mercury, cadmium and lead in soil and sediment materials
Developed methodology based on simultaneous IDMS and comparison of isotope ratios in ‘twin-blends’ is powerful tool in two-track certification of trace element content in CRMs with matrix requiring total digestion and different extraction procedures.
Real-time monitoring of the hydrolysis of vegetable oils loaded in silica colloidal crystal films with lipase by optical interferometry
The hydrolysis of four kinds of vegetable oil was monitored in real-time with ordered porous layer interferometry, which can be used to evaluate the health value of vegetable oil.
Synthesis and evaluation of a Zn–Al layered double hydroxide for the removal of ochratoxin A. Greenness assessment
The retention behavior of a dangerous toxin, ochratoxin A (OTA), present in food samples and derivatives was evaluated using Layered Double Hydroxides (LDHs).
Derivatization-assisted immunoassays: application for group-specific detection of potent methamphetamine and amphetamine enantiomers
Chemical derivatization into larger molecules has generated a monoclonal antibody that enables group-specific immunochemical detection of potent methamphetamine and amphetamine enantiomers.
Dual-mode chemosensor for the fluorescence detection of zinc and hypochlorite on a fluorescein backbone and its cell-imaging applications
Dual mode chemosensor fluorescein coupled with 3-(aminomethyl)-4,6-dimethylpyridin-2(1H)-one (FAD) has been reported for simultaneous detection of Zn2+ and OCl− by metal coordination and chemodosimetric approach respectively.
A stir bar sorptive extraction device coupled with a gas chromatography flame ionization detector for the determination of abused prescription drugs in lean cocktail samples
This work presents an in-house developed stir bar sorptive extraction (SBSE) device with an XAD-2 adsorbent followed by analysis using GC-FID for the extraction and determination of the five main abused prescription drugs in lean cocktail samples.
Clinical evaluation of an innovative isothermal amplification detection system for COVID-19 diagnosis
A sensitive, specific, and convenient method was developed for SARS-CoV-2 detection using a hot-pack-applied isothermal nucleic acid amplification method.
A carbon-black-embedded poly(dimethylsiloxane)-paper hybrid device for energy-efficient nucleic-acid amplification in point-of-care testing
A paper-based device patterned with a carbon-black–poly(dimethylsiloxane) (PDMS) mixture is developed as a heating platform for nucleic-acid amplification tests.
Rapid detection of phycotoxin domoic acid in seawater and seafood based on the developed lateral flow immunoassay
A lateral flow immunoassay (LFIA) of phycotoxin domoic acid (DA) contaminating seawater and marine organisms was developed in this investigation.
A novel sensor for visual and selective detection of Hg2+ based on functionalized doped quantum dots
A novel electrochemiluminescence sensor and visual attempts for mercury ion detection were developed by using new functionalized doped quantum dot nano-materials.
Study on the activity of Huo–Xue–Hua–Yu decoction and its drug groups in improving fracture healing using surface-enhanced Raman scattering (SERS) spectroscopy based on gold nanoparticles
The SERS technique has great potential for providing a novel method for effectively and accurately studying the efficacy of the Huo–Xue–Hua–Yu decoction and its drug groups in improving fracture healing.
Multi-wavelength fusion column fingerprint technology combined with chemometric analysis to evaluate the overall quality of the Gardenia jasminoides root
Chromatographic fingerprinting provides effective technical means for quality evaluation of traditional Chinese medicine.
An electrochemical biosensor based on few-layer MoS2 nanosheets for highly sensitive detection of tumor marker ctDNA
A method based on few-layer MoS2 nanosheets for highly sensitive electrochemical detection of tumor marker ctDNA.
Evaluation of laser direct infrared imaging for rapid analysis of pharmaceutical tablets
Chemical imaging is an important tool for characterising the distribution of components within pharmaceutical tablets. LDIR imaging has shown to be a promising technique for providing high-definition component distribution maps at an order of magnitude faster than traditional methods.
A SERS pH sensor for highly alkaline conditions and its application for pH sensing in aerosol droplets
A SERS pH sensor employing Alizarin Yellow R as the molecular probe was developed for use in strong alkaline solutions.
Simple manipulation of enzyme-linked immunosorbent assay (ELISA) using an automated microfluidic interface
ELISA on microfluidic interface device for lipoarabinomannan.
Fabrication of paper-based SERS substrate using a simple vacuum filtration system for pesticides detection
A paper-based SERS substrate with numerous ‘hotspots’ was fabricated by a simple vacuum filtration method, exhibiting excellent SERS performance for analytes detection.
Method for extraction and analysis of per- and poly-fluoroalkyl substances in contaminated asphalt
The legacy use of aqueous film-forming foam (AFFF) has led to the generation of large volumes of per- and poly-fluoroalkyl substances (PFAS)-contaminated asphalt materials, especially at airports and fire training areas.
Cationic AIEgen micelle-improved chemiluminescent H2O2 assay by integrating reactant approach and CRET
A two-in-one cationic AIEgen micelle-boosted chemiluminescent H2O2 assay with improved sensitivity is presented.
Single-cell patterning regulation by physically modified silicon nanostructures
A method for fabricating physically modified silicon nanostructures by combining double-beam double exposure interference lithography with MACE was developed to study the behavior and localization efficiency of SHSY5Y cells on the nanostructures.
A simple and rapid approach for on-site analysis of nicotine in tobacco based on a screen-printed electrode as an electrochemical sensor
In this study, we report a portable kit consisting of a portable workstation, gold screen-printed electrode (SPE), 0.45 μm filter membrane, phosphate buffer solution (PBS), and acetic acid (1%) for point-of-use (POU) analysis of nicotine in tobacco.
Two-stage nicking enzyme signal amplification (NESA)-based biosensing platform for the ultrasensitive electrochemical detection of pathogenic bacteria
An electrochemical biosensing platform for S. typhimurium analysis on the basis of Nt.Bsmal-assisted two-stage signal amplification.
A critical comparison between an ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QqQ-MS) method and an enzyme assay for anti-cholinesterase pesticide residue detection in cereal matrices
Critical comparison of two in-house analytical methods based on different principles for the detection of pesticide residues in cereals.
Preconcentration of sulphonamides in bovine milk by the cloud point extraction method using smartphone-based digital images
The procedure does not require organic solvents and is based on easy CPE of sulphonamides from milk samples. Established limits for total sulphonamides in milk were met using digital images.
An ultra-fast, one-step RNA amplification method for the detection of Salmonella in seafood
Salmonella is one of the most common pathogens associated with food-borne illness resulting from seafood consumption.
Mixed-mode chromatography-mass spectrometry enables targeted and untargeted screening of carboxylic acids in biological samples
Mixed-mode chromatography method offers quantification of TCA carboxylates in cells, human plasma, and plants.
Construction of a colorimetric sensor array based on the coupling reaction to identify phenols
We report a colorimetric sensor array, which uses two nanozymes as electronic tongues for fingerprint identification of six phenols. The six phenols at 50 nM have their own response patterns. The sensor array had distinguished the six phenols in actual samples successfully.
Exploring the problem of determining human age from fingermarks using MALDI MS-machine learning combined approaches
Framework employed to determine human age.
A luminescent Zn-MOF for the detection of explosives and development of fingerprints
A luminescent MOF (PUC1) was synthesized and applied as chemical sensor for explosives such as PETN and Tetryl with 0.315 and 0.404 μm detection limit.
Smartphone imaging spectrometer for egg/meat freshness monitoring
A reliable smartphone imaging spectrometer developed foron-site food freshness testing offers portability, CMOS compatibility, enhanced near-infrared spectrum capture capability and on-the-go (OTG) functionality.
Investigating protein diffusivities in diluted hyaluronic acid solutions using dynamic light scattering
A method based on analyzing the autocorrelation function of the dynamic light scattering is developed to investigate protein diffusivities in diluted protein/solvent binary systems and diluted protein/hyaluronic acid/solvent ternary systems.
About this collection
This themed collection features articles published in Analytical Methods marked as HOT by the handling editor or as recommended by referees. Congratulations to all the authors whose articles are featured.