Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification

Yanchen Chi; Meng Shi; Yanfang Wu; Yunping Wu; Yangyang Chang; Meng Liu

doi:10.1039/D2AY00656A

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Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification†

Yanchen Chi,‡^a Meng Shi,‡^a Yanfang Wu,

^b Yunping Wu,^a Yangyang Chang^a and Meng Liu

*^a

Author affiliations

* Corresponding authors

^a School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian POCT Laboratory, Dalian, China
E-mail: mliu@dlut.edu.cn

^b School of Chemistry and Australian Centre for Nano Medicine, The University of New South Wales, Sydney, NSW, Australia

Abstract

We described a new system termed droplet DNAzyme-coupled rolling circle amplification (dDRCA) that can selectively detect bacteria from clinical urine samples with single-cell sensitivity within 1.5 h compared with the several hours needed for traditionally used culture-based methods.

This article is part of the themed collection: Analytical Methods HOT Articles 2022

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DOI: https://doi.org/10.1039/D2AY00656A
Article type: Communication
Submitted: 20 Apr 2022
Accepted: 11 May 2022
First published: 12 May 2022

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Anal. Methods, 2022,14, 2244-2248

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Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification

Y. Chi, M. Shi, Y. Wu, Y. Wu, Y. Chang and M. Liu, Anal. Methods, 2022, 14, 2244 DOI: 10.1039/D2AY00656A

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Analytical Methods

Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification†

Abstract

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Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification

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