Themed collection Analytical Methods Review Articles 2025

Circulating tumour DNA (ctDNA) methylation analysis, a noninvasive liquid biopsy technique, has shown great potential in cancer diagnosis and treatment.

This review explores untargeted metabolomics in sports nutrition, outlining analytical workflows, data processing, and key applications in exercise, training, and dietary studies, while addressing issues of reproducibility and clinical translation.

The article mainly introduces recent advances in ion resonance methods such as isolation, CID, and scanning.

The novelty of this review lies in the comprehensive exploration of hydrogel-assisted chemiluminescence and electrochemiluminescence systems for bioanalytical applications.

CQDs enhance electrochemical sensing by improving electron transfer, selectivity, and catalytic activity. Their integration into sensors enables ultra-sensitive detection of metal ions, biomolecules, and environmental pollutants.

Diabetes mellitus is a disease that affects a large number of people around the world.

This review explores and evaluates novel tear collection methods designed to overcome the limitations of traditional techniques, including issues related to patient discomfort, contamination risks, and procedural inefficiencies.

Artificial Intelligence (AI) combined with non-invasive biochemical sensing is transforming healthcare monitoring and diagnostics.

DESI-MS is an ambient ionization, rapid, and high-throughput molecular analysis directly from surfaces. Recent advancements enhance its performance in imaging and diagnostics.

β-Propiolactone: from viral inactivation in vaccines to sensitive and specific assays for monitoring residual levels with GC and HPLC assay methods.

This review summarizes current evidence on low-cost particulate matter (PM) sensors for indoor and occupational environments and proposes a framework that links performance evaluation, calibration, and uncertainty to decision-making.

A review of organic probes for chemo/bio-sensors is summarized, focusing on chemical and crystal strcutures. It discusses various probes, including small organic molecules, MOFs, COFs, polymers, dye@nanoparticles, and dye-modified polymers.

Schematic illustration of diverse spectroscopic approaches for detecting AKI and CKD biomarkers, including colorimetric, fluorescence, SERS, and CL methods.

Artificial intelligence (AI) and machine learning (ML) in capillary electrophoresis (CE); AI– ML – optimization – CE; artificial intelligence – ML-CE for real samples; current challenges, strategic recommendations and future perspectives.

Ionistation association varies with the solvent dielectric constant, added salt, temperature and pressure for discrete electrostatic binding between bulky ions.

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death due to late diagnosis, high recurrence rate and poor response to systemic therapy.

Foodborne pathogenic bacterial contamination poses a major challenge to global food safety and public health, making the development of rapid, sensitive, and specific detection technologies critically important.

Nano-electrochemical sensors enable rapid, accurate detection of crop viruses. Advances in design, recognition, and portable integration are reviewed, with focus on applications, challenges, and prospects for sustainable agriculture.

Non-invasive lactate monitoring is crucial for athletes. This review explores electrochemical biosensors for sweat, saliva, and ISF, focusing on sensing principles, materials, architectures, performance, and challenges with emerging solutions.

This review highlights nanosensors for on-site detection of Pb, Cd, and As in soil, covering electrochemical and optical methods, portable integration, and future AI/ML-enabled precision agriculture applications.

This review examines wearable chemical sensors for workplace exposure monitoring, covering sensor types, validation, data management, regulatory challenges, and future directions toward adoption.

This review surveys recent progress in electrochemical aptasensors for organic pollutants, covering sensor design, aptamer choice, transduction, applications, performance, and real-sample use, with future prospects in stability and portability.

Recently developed CRISPR/Cas-based detection strategies, multiplex detection strategies by the combination of different CRISPR/Cas and challenges as well as prospects for tumor biomarker detection were comprehensively summarized and discussed.

Methamphetamine (METH) is one of the most widely abused illicit synthetic drugs globally, necessitating the development of rapid detection technologies.

This figure mainly shows the proportion of papers studying the content of metal elements in water environment, which well demonstrates the necessity of the research in this paper.

Gas chromatography-ion mobility spectrometry (GC-IMS) is an emerging analytical technique with significant potential for natural medicine analysis because of its high sensitivity, resolution, rapid detection, and portability.

Recent progress on aptamer-based biosensors for tobramycin are summarized.

This review critically explores analytical techniques, such as GC-MS, FTIR, NMR, and mechanical tests used to characterize fertilizer coatings and assess caking behavior, offering new insights for product stability and quality control.

This review summarizes the recently reported urea and thiourea-based small molecular chromofluorogenic chemosensors utilized for detecting diverse metal ions and anions.

Dengue, a major global health risk, is a virus spread by Aedes aegypti and Aedes albopictus mosquitoes.

This review summarizes advances in cyclodextrin-based hybrid materials for electrochemical sensing, focusing on molecular recognition, fabrication strategies, and prospects for selective, high-performance, and reusable sensors.

This review explores state-of-the-art 3D-printed electrochemical sensors for environmental monitoring, highlighting advanced materials, manufacturing approaches, and improved detection capabilities.

This critical review summarizes and discusses e-cigarette aerosol sampling methods for the quantification of metals and metalloids.

This review explores emerging CRISPR-microfluidic platforms enhancing precision, speed, and portability for point-of-care diagnostics. Innovations like SHINE, CARMEN, ITP, DNAiTECH, Dμchip, FAST, and MAPnavi highlight their potential.

Owing to its advantages such as high sensitivity, Raman spectroscopy can rapidly provide molecular information of samples and has thus been extensively applied in the realm of nutritional science.