Themed collection Analyst HOT Articles 2023

This review comprehensively discusses the methods and applications of various microfluidic functionalities for implantable medical devices.

The research progress and applications of colorimetric sensors based on nanozymes with peroxidase-like activity.

This review provides a broad overview of mass spectrometry-based proteomics and highlights four developing areas: (1) protein inference, (2) alternative proteases, (3) sample-specific databases and (4) post-translational modification discovery.

Mass spectrometry imaging (MSI) has advanced from a niche technique to a widely applied spatial biology tool. This review highlights state-of-the-art MSI application in emerging and the six largest biomedical fields according to a set of criteria.

Recently, surface plasmon resonance (SPR) biosensors have been widely used in environmental monitoring, food contamination detection and diagnosing medical conditions due to their superior sensitivity, label-free detection and rapid analysis speed.

The breast tumor microenvironment, preclinical breast cancer models, fabrication methods, breast tumor-on-chip models, and applications are summarized.

Modifications of electrodes for trace metal analysis reported in the last five years are summarized. Nanomaterials, polymers, and film-forming substances are the most used modifiers.

This review outlines the overview, advances and opportunities of genetically encoded fluorescence lifetime biosensors, including their combination of lifetime imaging technique and lifetime analysis methods, applications and future consideration.

This review focus on highlights the recent advances in wearable sensor toward continues and monitoring the risk factors of metabolic syndrome (MS).

Review of the ability of high resolution mass spectrometry to detect, type, subtype and distinguish variants and sub-variants in virus clinical specimens with high confidence.

The present review presents the advances in ratiometric pH probes development for ΔpHi mapping in cellular organelles. Furthermore, we highlight the major challenges in developing ratiometric probes that merit further research in the future.

A schematic diagram of the main methods for hepatitis B detection.

The cell is the most basic structural unit and plays a vital role in the function of an organism.

This review summarizes recent advances on metal-organic frameworks for the applications in photoelectrochemical and electrochemiluminescence biosensors.

This review focuses on the theoretical concepts, electrode engineering, data analytics, and biomedical applications of electric and electrochemical microfluidic platforms, and highlights future directions for development.

Development of photoelectrochemical sensors interfaced with semiconductor quantum dots (QDs) as a promising approach for fabricating new photoelectrodes and photoelectrochemistry systems suitable for energy storage and biosensing applications.

Typical forms of a combination of electrowetting-on-dielectric (EWOD) technology with sensing and other microfluidic manipulation techniques are reviewed from a technical perspective, including the purposes, significance, and biomedical applications.

We developed fluorescent probes that rapidly optimize linkers using the strong heterodimer formation of the peptide pair to generate biosensors with high response.

This research highlights in vivo significance of 50 nm PEGylated Si NPs, which were confirmed by obtaining satisfactory hyperpolarized ²⁹Si MR images.

We developed a system to separate and identify racemised and isomerised aspartic acid residues in amyloid β by labeling with an original chiral resolution labeling reagent, D-FDLDA.

Turn-on small molecule fluorescence sandwich immunoassay.

As a folate antagonist, methotrexate (MTX) has been widely used in clinics with good effects on various tumors and inflammatory diseases.

A conductive phthalocyanine-based porous organic polymer (NiPc-CC POP) was synthesized and used as a conductive catalyst for electrochemical detection of Van with good performance.

A point-of-care electrochemical biosensor was designed for Escherichia coli 16S rRNA analysis by coupling a screen-printed carbon electrode and MoS₂-based nanoprobes with high sensitivity and selectivity.

Raman image super-resolution was performed by a locality enhanced and lightweight transformer network, accelerating high-quality Raman imaging.

This work describes an ECL-biosensing method that incorporates a disposable ECL capillary-fill device with a peptide-cleavage strategy for the determination of the biomarker PSA, which can eliminate the light-shielding of MBs on the ECL biosensor.

Converting plastic waste into value-added electrochemical materials for hazardous PPD detection.

A pioneering ds-GapM-LNA nanobiosensor for the monitoring of long non-coding RNA (lncRNA) expression in live cells during the osteogenic and adipogenic differentiation of human mesenchymal stem cells (hMSCs).

1 is the first mitochondrion-targeted fluorescent probe based on ESIPT phthalimide for the detection of Hg²⁺ with large Stokes shift, rapid response, and high sensitivity and selectivity.

This work introduces the in-depth analysis of glycopeptides in human serum using a novel magnetic COF@MOF with a phosphoserine anchor.

A signal-enhanced fluorescence analysis method for rapid and sensitive detection of the pesticide residue TM was established based on the strong affinity between TM molecules and CuI CPs.

A simple and robust fluorescence strategy based on a nontarget DNA-triggered catalytic hairpin assembly (CHA) for the improved detection of microRNA-21 in a complex matrix is reported.

AgNCs-hydrogel can promote the regeneration of diabetic infectious wounds by regulating some key proteins.

Hairpin DNA-based electrochemical amplification strategy for miRNA sensing by adopting single gold nanoelectrodes.

In this work, by ingeniously designing the splicing junction-specific stem-loop DNA probe pair, we proposed a one-pot ligation-dependent loop-mediated isothermal amplification strategy (one-pot ligation-LAMP) for detecting splicing variants.

We report a photoluminescent and electrochemiluminescent Hg²⁺ probe based on the transmetalation reaction between Hg²⁺ and boronic acid of the ancillary ligand of a cyclometalated Ir(III) complex.

Optical-photothermal infrared spectra and discrete wavenumber images of single cells in an aqueous buffer were collected from six cell types. Classification models were trained and validated based on 7895 spectra from 1579 cells.

Using a light-up aptamer that can bind doxycycline, the binding thermodynamics and kinetics of the full-length aptamer and a series of split aptamers are compared.

A small-molecule fluorescent probe has been developed for detecting mycotoxin patulin in fruit juices. It enables convenient visual detection of patulin in juices made from decayed fruits, thus ensuring food safety in fruit-based products.

Underivatized oligopeptides can be detected by parahydrogen hyperpolarized NMR in a biofluid sample. The method is supported by an experimental and DFT study of the underlying oligopeptide complexes with the iridium-based hyperpolarization catalyst.

Based on the boronate affinity interaction between boric acid and cis-diols, 6-borono-1-methyl-quinoline-1-ium (BMQI) was designed and used as a reactive matrix for in situ derivatisation and rapid profiling of cis-diol compounds in MALDI-MS.

A novel colorimetric and ratiometric supramolecular probe is developed for the on-site detection of FPN with a fast response, high sensitivity, and good selectivity and anti-interference performance.

Annotation of common and new amino acid-conjugated bile acids from LC-MS/MS data is achieved by an automatic workflow using a semi-empirical MS/MS library in negative ESI mode and characteristic fragments in positive ESI mode.

Fast and visual detection of dichloroacetonitrile in water.

The N-/O-glycosylation and site-specific mapping of sialic acid linkage isomers of hACE2 were characterized, and which might give the clue for illustrating of the interaction between host cell and infection virus.

A homogeneous photoelectrochemical sensor based on 3D ZnO/Au/2D MoS₂ is proposed for the ultrasensitive detection of tetracycline (TET).

Four structurally well-defined chiral stationary phases of allylimidazole cyclodextrin derivatives were prepared for enantioseparation to explore the role of cyclodextrin hydroxyl groups.

A DNA hydrogel film is combined with capillary self-driven action for the specific detection of the tumor marker EpCAM, and Exo I is further introduced for signal amplification.

Ambient ion funnel combined with electrospray enables direct, intact, large-area, high-density and uniform deposition of nanoparticles, offering a general approach to fabricate high-performance substrates for surface-enhanced Raman scattering (SERS).

We speed up Raman imaging by: using a bright field image to skip sample background regions; using matrix completion to sub-sample without sacrificing resolution; using “low resolution Raman” to improve SNR without sacrificing chemical specificity.

A self-assembled L-cysteine nanosensor was constructed based on the FRET effect, indicating the potential of the probe in practical applications.

We created novel Pd/CB-Ni@rGO nanomaterials for glucose detection.

A Ti⁴⁺-functionalized magnetic covalent organic framework material with flexible branched polymers (mCOF@ε-PL@THBA-Ti⁴⁺) was proposed through post-synthesis modification.

Metal–organic frameworks (MOFs) show excellent catalytic activity and have been widely applied in diagnosis of diseases and tumors.

A photoelectrochemical aptasensor was constructed for ultrasensitive TNF-α assay based on the signal generator of hollow CdS cubes and the signal extinguishing activity of Ni₂Co₂O₄-Au.

CoVariant arrays consist of spike variants of SARS-CoV-2. We profiled plasma neutralizing and binding antibodies in Kawasaki disease and healthy with unvaccinated, vaccinated, or infected with CoVariant arrays to compare their humoral responses.

Two electrochemical sensors are proposed here for the first time for the fast screening of cannabinoids in Cannabis sativa L. plant material (inflorescences).

A novel design of dsDNA block contained a programmable sensing site inside is proposed, which can be programmed with different sequences to respond to multiple target molecules (e.g. Pb²⁺ and cfDNA) by being cleaved into two smaller DNA blocks.

An eco-friendly fluorescent probe (nano-Cdots) was developed for the detection of meloxicam in biological matrices based on the cooperative mechanisms of inner filter effect and photoelectron transfer.

Machine learning of urine and serum metabolic patterns encodes the discrimination and classification of autoimmune diseases. The selected metabolite panel, metabolite dysregulation and disturbance pathways were investigated.

CRISAT is a one-tube dual-readout biosensor developed by harnessing CRISPR-ALP tandem assay. This sensor has great potential for the detection of nucleic acids and non-nucleic acids.

A mitochondria-targeted near-infrared fluorescent probe was developed for selective and sensitive detection of ONOO⁻ both in vitro and in vivo.

Novel microfluidic SERS immunoassay to detect Plasmodium falciparum (Pf), with a LOD of 0.0012 ± 0.0001% parasitaemia, equivalent to ≈69 parasites per μL, a ∼7-fold higher sensitivity than most Malaria rapid diagnostic tests.

Online breath monitoring performed in mechanically ventilated patients provides information for breath sampling guidelines to be employed on awake patients.

The 3D hot spots fabricated in capillary provide an ideal platform for achieving rapid and on-site Hg²⁺ detection in SERS.

Raman spectroscopy has potential to allow for inexpensive high throughput screening of e-liquids. Here, we present its ability to quantify PG : VG ratio and nicotine content, and discuss the method's advantages and limitations.

The Au-CDs nanocomposite with efficient photo-oxidation activity at neutral pH was constructed for the colorimetric assessment of H₂O₂.

Fluorescence detection of 4-nitrophenol, α-glucosidase activity and α-glucosidase inhibitors based on the inner filter effect on Si nanoparticles synthesized through a green method.

A label-free electrochemical aptasensor was established for the POCT of HER-2 based on Bi–Sb alloy materials (Bi–Sb AMs).

Raman spectroscopy approach based on dried droplets, for mixed UTI bacterial pathogens identification.