Themed collection Innovative Tools for Cancer Screening, Detection and Diagnostics
Cancer: a global concern that demands new detection technologies
Welcome to this Analyst themed issue on Innovative Tools for Cancer Screening, Detection and Diagnostics, guest edited by Steven A. Soper and Avraham Rasooly.
Liquid biopsy for detection of actionable oncogenic mutations in human cancers and electric field induced release and measurement liquid biopsy (eLB)
This paper will review the analytical strategies used to assess oncogenic mutations from biofluid samples. Clinical applications will also be discussed.
Aptamers: versatile molecular recognition probes for cancer detection
Aptamers as specific and sensitive probes for in vitro diagnosis of cancer cells, immunohistochemical (IHC) staining of tumor tissues, and in vivo imaging detection of tumors.
Biosensors and nanobiosensors for therapeutic drug and response monitoring
Review of different biosensors and nanobiosensors increasingly used in therapeutic drug monitoring (TDM) for pharmaceutical drugs with dosage limitations or toxicity issues and for therapeutic response monitoring.
Isolation and enrichment of circulating biomarkers for cancer screening, detection, and diagnostics
A liquid biopsy is more practical for real-time monitoring of disease progression than tissue biopsy.
Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics
We present an overview of current isolation, detection, and characterization methods of extracellular vesicles and their applications and limitations as a potential emerging biomarker in cancer management and their clinical implementation.
Cancer screening via infrared spectral cytopathology (SCP): results for the upper respiratory and digestive tracts
Instrumental advances in infrared micro-spectroscopy have made possible the observation of individual human cells and even subcellular structures.
Can we observe changes in mRNA “state”? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes
RNA binding proteins (RBP) regulate the editing, localization, stabilization, translation, and degradation of ribonucleic acids (RNA) through their interactions with specific cis-acting elements within target RNAs.
Microfluidics and cancer analysis: cell separation, cell/tissue culture, cell mechanics, and integrated analysis systems
Among the growing number of tools available for cancer studies, microfluidic systems have emerged as a promising analytical tool to elucidate cancer cell and tumor function.
Cancer biomarker discovery using DNA aptamers
Aptamers, as chemical antibodies, show great promise and power for cancer biomarker discovery and biomarker-based clinical applications.
Raman technologies in cancer diagnostics
This review explores Raman technologies with a particular emphasis on their utility for cancer diagnostic applications.
Can mid-infrared biomedical spectroscopy of cells, fluids and tissue aid improvements in cancer survival? A patient paradigm
This review takes a fresh approach from the patient perspective; offering insight into the applications of midinfrared biomedical spectroscopy.
Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics
Early detection and reliable diagnostics are keys to effectively design cancer therapies with better prognoses.
Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices
We explore emerging micro and nano-technology that isolates and detects exosomes and microvesicles in clinical samples for cancer diagnostics.
Microfluidics for research and applications in oncology
Cancer is currently one of the top non-communicable human diseases, and continual research and developmental efforts, particularly in microfluidics technology, are being made to better understand and manage this disease.
A universal tumor cell isolation method enabled by fibrin-coated microchannels
We found that the fibrin-immobilized surfaces on microchannels were able to capture both epithelial and mesenchymal tumor cells.
An electrochemical-TUNEL method for sensitive detection of apoptotic cells
An electrochemical-TUNEL method based on the fabrication of a CNT@PDA–FA three dimensional bio-interface was developed for cytosensors. By being coupled with a QD-based nanoprobe and electrochemical analysis, the sensor exhibited attractive performance in the detection of apoptotic cells.
Nanoparticles of gadolinium-incorporated Prussian blue with PEG coating as an effective oral MRI contrast agent for gastrointestinal tract imaging
Nanoparticles of gadolinium-incorporated Prussian blue show potential as a cellular T1-weighted oral MRI probe for imaging the gastrointestinal tract.
Wavenumber selection based analysis in Raman spectroscopy improves skin cancer diagnostic specificity
Wavenumber selection based analysis with variably-sized windows was proposed, which improves skin cancer diagnostic specificity at high sensitivity levels.
FTIR imaging of the 3D extracellular matrix used to grow colonies of breast cancer cell lines
Infrared imaging was applied to investigate a reconstituted basement membrane, known as Matrigel, in three-dimensional cell cultures.
Clinical evaluation of a novel microfluidic device for epitope-independent enrichment of circulating tumour cells in patients with small cell lung cancer
Evaluation of a novel microfluidic device for epitope-independent enrichment of circulating tumour cells using clinical samples from patients with SCLC.
Automated analysis of single cells using Laser Tweezers Raman Spectroscopy
In recent years, significant progress has been made into the label-free detection and discrimination of individual cancer cells using Laser Tweezers Raman Spectroscopy (LTRS).
Rapid bladder cancer cell detection from clinical urine samples using an ultra-thin silicone membrane
A high-throughput strategy capable of differentiating bladder cancer cells from non-cancerous cells based on their respective cellular traction forces.
Real-time imaging of cancer cell chemotaxis in paper-based scaffolds
An easy to assemble paper-based invasion assay to study chemotaxis of breast cancer cells in gradients of oxygen in real-time.
High-resolution FTIR imaging of colon tissues for elucidation of individual cellular and histopathological features
Comparison of spectral-histopathological features of a colon tissue measured using a conventional (5.5 μm × 5.5 μm, left) and a high-magnification (1.1 μm × 1.1 μm, right) FTIR imaging system with respect to HE stained tissue (middle).
Grating coupled SPR microarray analysis of proteins and cells in blood from mice with breast cancer
Grating couple surface plasmon resonance (GCSPR) microarrays can be used to analyze proteins and circulating tumor cells in mice.
Characterization of human breast cancer tissues by infrared imaging
FTIR imaging allows automated identification and quantification of breast tumor cells as well as investigating tumor-related stroma alterations.
Microfluidics for the detection of minimal residual disease in acute myeloid leukemia patients using circulating leukemic cells selected from blood
Microfluidic assay for the selection of circulating leukemic cells from peripheral blood for the early detection of minimal residual disease in acute myeloid leukemia patients.
Microfluidic compartments with sensing microbeads for dynamic monitoring of cytokine and exosome release from single cells
Monitoring activity of single cells has high significance for basic science and diagnostic applications.
Detection of DNA methyltransferase activity using allosteric molecular beacons
Abnormal DNA methylation patterns caused by altered DNA methyltransferase (MTase) activity are closely associated with cancer. Herein, using DNA adenine methylation methyltransferase (Dam MTase) as a model analyte, we designed an allosteric molecular beacon (aMB) for sensitive detection of Dam MTase activity.
Identification of a p53-based portable degron based on the MDM2-p53 binding region
A shortened portable degron based on a MDM2/p53 binding region that was capable of ubiquitination in cytosolic lysates was isolated as a new substrate for analytical detection of E3 ligase or proteasome activity.
Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)
IR-MALDESI polarity switching mass spectrometry imaging reveals differences in lipid distribution in hen ovarian cancer tissue.
Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate
Sensing of methotrexate at clinically-relevant concentrations was achieved with a plasmon-coupling assay.
ATR-FTIR spectroscopy coupled with chemometric analysis discriminates normal, borderline and malignant ovarian tissue: classifying subtypes of human cancer
Spectrochemical discrimination of ovarian cancer.
About this collection
This themed collection showcases research on new technologies focused on the management of cancer-related diseases and covers a broad spectrum of new innovations including optical sensors, drug delivery vehicles, affinity agents, imaging contrast agents, microfluidics/nanofluidics and cell-based assays. Guest Edited by Steven A. Soper and Avraham Rasooly. New articles will be added to this collection as they are published.