Themed collection Most popular analytical chemistry articles

Droplet microfluidics constitutes a diverse and practical tool set that enables chemical and biological experiments to be performed at high speed and with enhanced efficiency when compared to conventional instrumentation.

Metal–organic frameworks with enzyme-like catalytic features (MOF nanozymes) exhibit great promise in detecting various analytes with amplified signal outputs.

This feature article comprehensively summarizes the development of small-molecule fluorescent probes for specific detection and imaging of chemical species inside lysosomes.

Key aspects, current trends, and future perspectives with respect to electrochemical affinity biosensors for clinical diagnosis, prognosis, and therapeutic actions are discussed.

A simple ratiometric sensor based on Raman spectroscopy enables rapid fluoride ion detection in a paper-based assay using a portable spectrometer.

A dual-ratiometric fluorescent probe, Han-HClO-H₂S, was developed for the individual and continuous detection of H₂S and HClO with high sensitivity and good selectivity, and had been applied to detect intracellular H₂S and/or HClO in living cells.

A novel hepatocyte-targeting near-infrared ratiometric fluorescent probe named Gal-NIR is developed for detecting ONOO⁻. The probe can target the hepatocyte and assess drug-induced liver injury and its remediation in living cells and mice.

An aptamer recognition-trigged label-free homogeneous electrochemical strategy was developed for highly selective and ultrasensitive detection of cancer-derived exosomes.

A ratiometric two-photon fluorescent probe for HClO was deliberately constructed to reveal the generation of HClO in alcohol-induced liver injury.

A novel ratiometric electrochemiluminescence–electrochemical hybrid biosensor with high accuracy and reproducibility was fabricated for the ultrasensitive detection of miRNA-133a.

A FRET-based reversible fluorescent probe for sensing SO₂ and FA was designed. The probe was first used for imaging endogenous SO₂ and FA in vitro and in vivo. Moreover, we first found that the interaction of SO₂ and FA can reduce the cytotoxicity.

A NIR fluorescent probe based on Si-rhodamine dithiobenzoic acid lactone was used to selectively visualize HOCl during GEN-induced nephrotoxicity for the first time.

We report a fluorescent sensing platform for telomerase activity assay by coupling a 3D DNA walker with the MnO₂-UCNPs-based FRET system.

A novel hydrosoluble near-infrared fluorescent probe is applied to imaging and detection of endogenous tyrosinase in living cells, zebrafish and a mouse model.

An approach for rapid resonance assignments in proteins based on 2D ¹³C-detected NMR experiments combined with amino acid selective unlabeling.

A novel biosensing platform based on Ag(I) ion-enhanced or Ag nanoclusters (NCs)-quenched electrochemiluminescence (ECL) of CdSe quantum dots (QDs) was designed for versatile “on–off” assays of thrombin (TB) and miRNA.

A bright red-emitting flavonoid derivative was synthesized, which exhibited a large Stokes shift (Δλ > 150 nm) and high fluorescence quantum yields (ϕ_fl = 0.10–0.35).

We report a rare example of near infrared circularly polarized luminescence (NIR-CPL) in the 920–1050 nm region associated with the f–f transitions of Yb diketonate complexes bearing chiral ancillary ligands.

Fluorescent aggregates of a cyano-substituted phenylenevinylene derivative (R-1) have been used as a bimodal probe for the easy and fast detection of CO₂.

Ratiometric pH sensing in the physiological range of pH 6.5–10.3 by a white light emitting quantum dot complex – following the changes in luminescence intensity ratio, color and chromaticity – is described herein.

A novel DNA hydrogel-amplified versatile fluorescence platform combined with hybridization chain reaction (HCR) and DNA walking multiple amplification was developed for ultrasensitive detection of miRNA.

An efficient fluorescence turn-on probe for amyloid detection in the extreme near-infrared region (>750 nm) with an exceptional red-shift in the absorption maxima is reported.

Fe₃O₄@COFs served as an adsorbent and new matrix for SALDI-TOF-MS analysis of PAHs and their derivatives in PM_2.5 with clear background, good reproducibility and sensitivity.

Pyrene–benzothiazolium probe for visualizing lysosome in live cells.

An exosomes–zirconium–liposomes sandwich structure is proposed to detect exosomes by using zirconium–phosphate coordination chemistry with lower cost, no modified label, and simplicity.

A lysosome-targeting dual-functional fluorescent probe was rationally designed and developed for imaging intracellular lysosomal viscosity and beta-amyloid.

A colorimetric and fluorescent probe ER-ClO was developed to detect cellular hypochlorite with high selectivity and sensitivity.

A dual functional fluorescent molecular rotor was developed to trigger intracellular ER autophagy and quantify the local viscosity variations by FLIM imaging.

Owing to the extreme toxicity of mercury, methods for its selective and sensitive sensing in solutions, and in live cells and tissues are in great demand.

A ratiometric two-photon fluorescent probe (Lyso-MPCB) was developed for visualizing autophagy by real-time imaging the fluctuation of lysosomal pH.