Guest editors G. V. Shivashankar, Michael Sheetz and Paul Matsudaira introduce the Mechanobiology themed issue of Integrative Biology.
Multiscale mechanobiology: computational models for integrating molecules to multicellular systems
We review recent computational approaches and supporting experimental work in mechanobiology across multiple scales, from molecules to multicellular systems.
Regulation of epithelial cell organization by tuning cell–substrate adhesion
Combining live cell imaging, particle image velocimetry and numerical simulations, we show the role of extracellular matrix and intercellular adhesion on the expansion of epithelial cells.
Actin cytoskeleton differentially alters the dynamics of lamin A, HP1α and H2B core histone proteins to remodel chromatin condensation state in living cells
Cellular mechanical signals via the actin cytoskeleton regulate DNA packing by altering the dynamics of nuclear structure proteins.
Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells
Micropatterning of T cell ligands reveals their specific contributions to actin assembly and the generation of cytoskeletal forces in immunesynapse formation.
Single cell super-resolution imaging of E. coli OmpR during environmental stress
We used super-resolution microscopy to visualize the bacterial transcriptional regulator OmpR, the bacterial membrane and the chromosome.
Single molecular force across single integrins dictates cell spreading
Molecular forces via single integrins at the cell-substrate interface control the degree of cell spreading.
Mechanisms of integrin and filamin binding and their interplay with talin during early focal adhesion formation
Mechanisms of the interplay among filamin, integrin and talin during early focal adhesion formation were explored using molecular dynamics simulations.
Modelling wound closure in an epithelial cell sheet using the cellular Potts model
We use a two-dimensional cellular Potts model to represent the behavior of an epithelial cell layer and describe its dynamics in response to a microscopic wound.
Modeling the finger instability in an expanding cell monolayer
Curvature-controlled cellular forces at the edge of an expanding monolayer are sufficient for the initiation and growth of finger-like instability.
Ratio of total traction force to projected cell area is preserved in differentiating adipocytes
During obesity development, preadipocytes proliferate and differentiate into new mature adipocytes, to increase the storage capacity of triglycerides.
Deterministic control of mean alignment and elongation of neuron-like cells by grating geometry: a computational approach
Combinatorial simulations are able to relate alignment and elongation of neuron-like cells on gratings.
Traction stress analysis and modeling reveal that amoeboid migration in confined spaces is accompanied by expansive forces and requires the structural integrity of the membrane–cortex interactions
Neutrophil-like cells, confined between two non-fibronectin-coated gels, form blebs and generate expansive forces against opposing surfaces during amoeboid cell “chimneying”.
Quantifying cell-induced matrix deformation in three dimensions based on imaging matrix fibers
An experimental technique that images fibers of an extracellular matrix to quantify cell-induced deformations and microstructural changes in three dimensions.
Non-monotonic cellular responses to heterogeneity in talin protein expression-level
Correlative imaging in single-cells of both live migration and post-fixation talin-labeling revealed non-monotonic correspondences between cellular properties and talin expression-levels.
Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration
Human tumors are stiff and data suggest that the extracellular matrix stiffening is consistent with experimental mammary tumor models in which stiffness drives tumor invasion and metastasis.
Oxygen governs gonococcal microcolony stability by enhancing the interaction force between type IV pili
Bacterial cell clusters disassemble after having depleted oxygen because the interaction force between their surface appendages is reduced.
Thermodynamics of force-dependent folding and unfolding of small protein and nucleic acid structures
A theoretical framework for understanding equilibrium mechanical stretching of single proteins.
Microtubule appendages mediating T-cell motility and polarity
Motility and polarity of T cells are mediated in large measure by differential adhesion through the long, flexible microtubule-based appendages.
Substrate elasticity modulates the responsiveness of mesenchymal stem cells to commitment cues
Fate choices of stem cells are regulated in response to a complex array of biochemical and physical signals from their microenvironmental niche.
High-throughput screening for modulators of cellular contractile force
Using cellular contractile force as the target, we report a new high-throughput screening technology.
The mechanotransduction machinery at work at adherens junctions
α-Catenin, the core mechanosensor of adherens junctions, allows cells to sense, transduce mechanical constrain in biochemical signals by force-dependent conformational changes regulating protein interactions.
About this collection
This themed issue on mechanobiology, guest edited by G. V. Shivashankar, M .P. Sheetz and P. Matsudaira, features original research and topical mini review articles on all areas of mechanobiology from molecular to organ scale, experimental, theoretical and computational analysis. Topics covered include: molecules and motors, mechanics of molecular motors in living cells, cell models of mechanobiology, mechanotransduction & genome regulation, mechanics of cellular differentiation/reprogramming, collective cell migration & tissue mechanics, mechanobiology of pathogenesis and cancer, bacterial mechanics and multiscale modeling in mechanobiology. We hope you enjoy this collection.